Results 161 to 170 of about 127,929 (201)
Some of the next articles are maybe not open access.
Discovery and characterization of a xylose reductase from Zymomonas mobilis ZM4
Biotechnology Letters, 2011Formation of xylitol, a byproduct from xylose fermentation, is a major limiting factor in ethanol production from xylose in engineered Zymomonas strains, yet the postulated xylose reductase remains elusive. We report here the discovery of xylose reductase in Zymomonas mobilis and, for the first time, to associate the enzyme function with its gene ...
Rachel Chen, Manoj Agrawal
exaly +3 more sources
Applied Biochemistry and Biotechnology, 2018
Xylitol is an important functional sugar alcohol which is widely used in industries. To efficiently produce xylitol from xylose by xylose reductase in a single step, the coupled system and the coexpression system using xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum were constructed, the latter ...
Yu-Guo Zheng, Zhiqiang Liu
exaly +3 more sources
Xylitol is an important functional sugar alcohol which is widely used in industries. To efficiently produce xylitol from xylose by xylose reductase in a single step, the coupled system and the coexpression system using xylose reductase (XR) from Rhizopus oryzae and glucose dehydrogenase (GDH) from Exiguobacterium sibiricum were constructed, the latter ...
Yu-Guo Zheng, Zhiqiang Liu
exaly +3 more sources
Multiple forms of xylose reductase in Pachysolen tannophilus CBS4044 [PDF]
FEMS Microbiology Letters, 1985 Johannes P van Dijken +2 more
exaly +2 more sources
Putative xylose and arabinose reductases in Saccharomyces cerevisiae
Yeast, 2002AbstractSaccharomyces cerevisiae mutants, in which open reading frames (ORFs) displaying similarity to the aldo‐keto reductase GRE3 gene have been deleted, were investigated regarding their ability to utilize xylose and arabinose. Reduced xylitol formation from D‐xylose in gre3 mutants of S. cerevisiae suggests that Gre3p is the major D‐xylose‐reducing
K L, Träff +2 more
openaire +2 more sources
Extraction by Reversed Micelles of the Intracellular Enzyme Xylose Reductase
Applied Biochemistry and Biotechnology, 2001Xylose reductase enzyme (EC 1.1.1.21) produced by Candida guilliermondii in sugarcane bagasse was extracted by reversed micelles of N-benzyl-N-dodecyl-N-bis (2-hydroxyethyl) ammonium chloride cationic surfactant. An experimental design was employed to evaluate the influences of the following factors on the enzyme extraction: temperature, cosolvent, and
E V, Cortez +4 more
openaire +2 more sources
Journal of Biotechnology, 2012
Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and ...
Sung-Haeng, Lee +3 more
openaire +2 more sources
Efficient conversion of xylose to ethanol is an essential factor for commercialization of lignocellulosic ethanol. To minimize production of xylitol, a major by-product in xylose metabolism and concomitantly improve ethanol production, Saccharomyces cerevisiae D452-2 was engineered to overexpress NADH-preferable xylose reductase mutant (XR(MUT)) and ...
Sung-Haeng, Lee +3 more
openaire +2 more sources
Xylose metabolism in Pachysolen tannophilus: purification and properties of xylose reductase
Canadian Journal of Microbiology, 1984Xylose reductase (xylitol: NADP oxidoreductase, EC 1.1.1.139) has been purified from D-xylose grown cells of the yeast Pachysolen tannophilus by application of DEAE-cellulose ion exchange chromatography, 2′,5′-ADP-Sepharose affinity chromatography, Biogel P200 gel filtration, and dextran blue Sepharose chromatography to approximately 95% homogeneity ...
Günther Ditzelmüller +3 more
openaire +1 more source
Biotechnology Journal, 2009
AbstractMetabolic engineering of Saccharomyces cerevisiae for xylose fermentation has often relied on insertion of a heterologous pathway consisting of nicotinamide adenine dinucleotide (phosphate) NAD(P)H‐dependent xylose reductase (XR) and NAD+‐dependent xylitol dehydrogenase (XDH).
Stefan, Krahulec +2 more
openaire +2 more sources
AbstractMetabolic engineering of Saccharomyces cerevisiae for xylose fermentation has often relied on insertion of a heterologous pathway consisting of nicotinamide adenine dinucleotide (phosphate) NAD(P)H‐dependent xylose reductase (XR) and NAD+‐dependent xylitol dehydrogenase (XDH).
Stefan, Krahulec +2 more
openaire +2 more sources
Optimization of Xylose Reductase production from Citrobacter sp.
Research Journal of Biotechnology, 2021Xylitol is a poly-hydroxy straight-chain five-carbon alcohol that can replace sugar in daily uses without any side effects. Lowered risk of dental carries and bone demineralization further support its involvement in a healthy lifestyle. In addition, its role in the synthesis of various commercial products like glycol, ethanol, and resins etc. increases
Rashmi Niranjan +2 more
openaire +1 more source
Yeast, 1996
A xylose reductase gene was isolated from the xylose-fermenting yeast Pachysolen tannophilus as a cDNA clone by selecting clones that hybridized specifically to xylose-inducible messenger RNA. Use of the cDNA clone as a probe in Northern hybridizations identified a xylose-inducible mRNA species large enough to encode a 36 kDa xylose reductase protein ...
P L, Bolen, G T, Hayman, H S, Shepherd
openaire +2 more sources
A xylose reductase gene was isolated from the xylose-fermenting yeast Pachysolen tannophilus as a cDNA clone by selecting clones that hybridized specifically to xylose-inducible messenger RNA. Use of the cDNA clone as a probe in Northern hybridizations identified a xylose-inducible mRNA species large enough to encode a 36 kDa xylose reductase protein ...
P L, Bolen, G T, Hayman, H S, Shepherd
openaire +2 more sources