Results 181 to 190 of about 7,409 (204)
Some of the next articles are maybe not open access.
Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis
Applied Microbiology and Biotechnology, 1988A low-affinity and a high-affinity sylose proton symport operated simultaneously in both starved and non-starved cells of Pichia stipitis. Glucose competed with xylose for transport by the low-affinity system and inhibited xylose transport by the high-affinity system non-competitively.
S. G. Kilian, N. van Uden
openaire +1 more source
Applied Microbiology and Biotechnology, 2008
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Ronald E, Hector +3 more
openaire +2 more sources
The goal of this investigation was to determine the effect of a xylose transport system on glucose and xylose co-consumption as well as total xylose consumption in Saccharomyces cerevisiae. We expressed two heterologous transporters from Arabidopsis thaliana in recombinant xylose-utilizing S. cerevisiae cells.
Ronald E, Hector +3 more
openaire +2 more sources
D-Xylose Transport by Candida succiphila and Kluyveromyces marxianus
Applied Biochemistry and Biotechnology, 2003The kinetics and regulation of D-xylose uptake were investigated in the efficient pentose fermentor Candida succiphila, and in Kluyveromyces marxianus, which assimilate but do not ferment pentose sugars. Active high affinity (Km approximately 3.8 mM; Vmax approximately 15 nmol/[mg min]) and putative facilitated diffusion low-affinity (Km approximately ...
Boris U, Stambuk +3 more
openaire +2 more sources
Mechanism of D-Xylose Transport in Human Small Intestine
Journal of Pediatric Gastroenterology and Nutrition, 1986Summary: The transport mechanism of D‐xylose across the mucosal border of small intestine was investigated in humans both in vitro and in vivo. A comparative study was also made between infants and adults. We investigated the Na+ dependence of D‐xylose influx from the mucosal solution into the epithelial cells, the phlorizin sensitivity of the influx ...
N, Ohkohchi +3 more
openaire +2 more sources
Understanding xylose transport in yeasts
Xylose constitutes the second major sugar fraction of the plant-derived lignocellulosic biomass, which is the most abundantly available and renewable feedstock for microbial fermentations. Hence, comprehensive utilization of xylose is crucial from the perspective of sustainable development of bio-based products, such as fuels, fine chemicals, and high ...Atrayee Chattopadhyay +2 more
openaire +2 more sources
Enzyme and Microbial Technology, 2014
Since the uptake of xylose is believed to be one of the rate-limiting steps for xylose ethanol fermentation by recombinant Saccharomyces cerevisiae strains, we transformed a hxt-null strain lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) with an integrative plasmid to overexpress the genes for xylose reductase (XYL1), xylitol ...
Davi L, Gonçalves +5 more
openaire +2 more sources
Since the uptake of xylose is believed to be one of the rate-limiting steps for xylose ethanol fermentation by recombinant Saccharomyces cerevisiae strains, we transformed a hxt-null strain lacking the major hexose transporters (hxt1Δ-hxt7Δ and gal2Δ) with an integrative plasmid to overexpress the genes for xylose reductase (XYL1), xylitol ...
Davi L, Gonçalves +5 more
openaire +2 more sources
An assay for functional xylose transporters in Saccharomyces cerevisiae
Analytical Biochemistry, 2013It has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. In this study, an assay to screen xylose transporters was established in the Saccharomyces cerevisiae strain, which expresses the xylosidase gene of Bacillus pumilus intracellularly.
Chengqiang, Wang +4 more
openaire +2 more sources
d-Xylose transport in the chicken small intestine
Comparative Biochemistry and Physiology, 1967Abstract 1. 1. d -Xylose enters rings of chicken small intestine, in vitro , significantly faster and to a greater extent than its epimer at carbon-2, d -lyxose. Final distribution of lyxose is indistinguishable from the extracellular space. 2. 2. Xylose, but not lyxose entry exhibits saturation kinetics. 3. 3.
openaire +2 more sources
Molecular Simulations of Solute Transport in Xylose Isomerase Crystals
The Journal of Physical Chemistry B, 2008Cross-linked enzyme crystals (CLECs) enclose an extensive regular matrix of chiral solvent-filled nanopores, via which ions and solutes travel in and out. Several cross-linked enzyme crystals have recently been used for chiral separation and as biocatalysts. We studied the dynamics of solute transport in orthorhombic d-xylose isomerase (XI) crystals by
Kourosh, Malek, Marc-Olivier, Coppens
openaire +2 more sources
A kinetic analysis of d-xylose transport in rhodotorula glutinis
Biochimica et Biophysica Acta (BBA) - Biomembranes, 1978The kinetics of D-xylose transport were studied in Rhodotorula glutinis. Analysis of the saturation isotherm revealed the presence of at least two carriers for D-xylose in the Rhodotorula plasma membrane. These two carriers exhibited Km values differing by more than an order of magnitude.
M E, Alcorn, C C, Griffin
openaire +2 more sources