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Construction of Yeast Surface-Displayed cDNA Libraries [PDF]
, 2011 Using yeast display, heterologous protein fragments can be efficiently displayed at high copy levels on the Saccharomyces cerevisiae cell wall. Yeast display can be used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties.
Scott, Bidlingmaier, Bin, Liu
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Journal of Biological Engineering, 2019 Background The promise of biopharmaceuticals comprising one or more binding domains motivates the development of novel methods for de novo isolation and affinity maturation of virion-binding domains.
Pete Heinzelman +4 more
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The cytoplasmic adaptor protein Caskin mediates Lar signal transduction during Drosophila motor axon guidance [PDF]
, 2011 The multiprotein complexes that receive and transmit axon pathfinding cues during development are essential to circuit generation. Here, we identify and characterize the Drosophila sterile α-motif (SAM) domain-containing protein Caskin, which shares ...
Broihier, Heather T +3 more
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A novel one-step approach for the construction of yeast surface display Fab antibody libraries
Microbial Cell Factories, 2018 Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as ...
Simon Rosowski +10 more
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mAbs, 2021 Therapeutic antibodies must have “drug-like” properties. These include high affinity and specificity for the intended target, biological activity, and additional characteristics now known as “developability properties”: long-term stability and resistance
Andre Azevedo Reis Teixeira +10 more
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Analysis of the transcriptional program governing meiosis and gametogenesis in yeast and mammals [PDF]
, 2006 During meiosis a competent diploid cell replicates its DNA once and then undergoes two consecutive divisions followed by haploid gamete differentiation.
Schlecht, Ulrich
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VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)
Frontiers in Bioengineering and Biotechnology, 2023 The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda +13 more
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mAbs, 2020 Antibody discovery using invitro display technologies such as phage and/or yeast display has become acornerstone in many research and development projects, including the creation of new drugs for clinical use.
Fortunato Ferrara +5 more
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Characterization of Genes Involved in the Formation of Aroma Volatiles in "Charentais" Melon Fruit [PDF]
, 2004 Volatiles esters impart distinct characteristics to the fruit quality. "Charentais" cantaloupe melon (Cucumis melo "cantalupensis") is characterized by abundant sweetness and aromatic flavour.
El-Sharkawy, Islam +4 more
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Molecular Display Technology Using Yeast—Arming Technology—
Analytical Sciences, 2009 A technology to display proteins or peptides on living organisms has been developed over the last two decades. So-called "Molecular display (Arming) technology" or "Cell surface engineering" has been one of the important tools for analyzing and understanding protein function, or for screening of novel clones from libraries. In addition, it endows cells
Seiji, Shibasaki +2 more
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