Results 21 to 30 of about 889 (213)
Toxins, 2014 Staphylococcus aureus and group A Streptococcus secrete a collection of toxins called superantigens (SAgs), so-called because they stimulate a large fraction of an individual’s T cells.
Preeti Sharma +2 more
doaj +1 more source
Journal of Biological Engineering, 2019 Background The promise of biopharmaceuticals comprising one or more binding domains motivates the development of novel methods for de novo isolation and affinity maturation of virion-binding domains.
Pete Heinzelman +4 more
doaj +1 more source
A novel one-step approach for the construction of yeast surface display Fab antibody libraries
Microbial Cell Factories, 2018 Background Yeast surface display (YSD) has proven to be a versatile platform technology for antibody discovery. However, the construction of antibody Fab libraries typically is a tedious three-step process that involves the generation of heavy chain as ...
Simon Rosowski +10 more
doaj +1 more source
Isolation of HCV Neutralizing Antibodies by Yeast Display [PDF]
, 2018 Yeast surface display (YSD) enables efficient screening and selection of single chain variable fragments (scFvs) of heavy (VH) and light (VL) chains that bind to target antigen with different affinities. Assembly of a scFv library from cDNA usually involves adding different primers and linkers (Gly4/Ser)3 through multiple rounds of PCR amplification ...
Zhen-Yong, Keck +3 more
openaire +2 more sources
mAbs, 2021 Therapeutic antibodies must have “drug-like” properties. These include high affinity and specificity for the intended target, biological activity, and additional characteristics now known as “developability properties”: long-term stability and resistance
Andre Azevedo Reis Teixeira +10 more
doaj +1 more source
mAbs, 2020 Antibody discovery using invitro display technologies such as phage and/or yeast display has become acornerstone in many research and development projects, including the creation of new drugs for clinical use.
Fortunato Ferrara +5 more
doaj +1 more source
VNAR development through antigen immunization of Japanese topeshark (Hemitriakis japanica)
Frontiers in Bioengineering and Biotechnology, 2023 The VNAR (Variable New Antigen Receptor) is the smallest single-domain antibody derived from the variable domain of IgNAR of cartilaginous fishes. Despite its biomedical and diagnostic potential, research on VNAR has been limited due to the difficulties ...
Hiroyuki Takeda +13 more
doaj +1 more source
High‐throughput screening and biological display technology: Applications in molecular imaging
iRADIOLOGY, 2023 Molecular imaging plays important roles in many fields, including disease diagnosis, therapeutic efficacy evaluation, intraoperative imaging guidance, drug metabolism monitoring, and patient selection for appropriate treatment.
Renli Luo, Hongguang Liu, Zhen Cheng
doaj +1 more source
Applications of Yeast Surface Display for Protein Engineering [PDF]
, 2015 The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications.
Gerald M, Cherf, Jennifer R, Cochran
openaire +2 more sources
Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?
FEBS Letters, EarlyView.This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes +3 more
wiley +1 more source