Results 11 to 20 of about 889 (213)
Journal of Extracellular Vesicles, 2021 The research of extracellular vesicles (EVs) has boomed in the last decade, with the promise of them functioning as target‐directed drug delivery vehicles, able to modulate proliferation, migration, differentiation, and other properties of the recipient ...
Stefan Vogt +7 more
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The Use of Yeast in Biosensing
Microorganisms, 2022 Yeast has been used as a model for several diseases as it is the simplest unicellular eukaryote, safe and easy to culture and harbors most of the fundamental processes that are present in almost all higher eukaryotes, including humans. From understanding
Sudip Dhakal, Ian Macreadie
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Evaluation of the yeast surface display system for screening of functional nanobodies
AMB Express, 2020 Yeast surface display is a powerful technology used to isolate and engineer proteins to improve their activity, specificity, and stability. In this method, gene expression is regulated by promoters, and secretion efficiency is affected by secretion ...
Kaho Kajiwara +2 more
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Microorganisms, 2023 In the long history of microorganism use, yeasts have been developed as hosts for producing biologically active compounds or for conventional fermentation.
Seiji Shibasaki, Mitsuyoshi Ueda
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Microbiology Spectrum, 2015 ABSTRACT Despite the availability of antimicrobial drugs, the continued development of microbial resistance—established through escape mutations and the emergence of resistant strains—limits their clinical utility. The discovery of novel, therapeutic, monoclonal antibodies (mAbs) offers viable clinical alternatives in the ...
Jared, Sheehan, Wayne A, Marasco
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Experimental evolution of a sexually selected display in yeast [PDF]
Proceedings of the Royal Society B: Biological Sciences, 2008 The fundamental principle underlying sexual selection theory is that an allele conferring an advantage in the competition for mates will spread through a population. Remarkably, this has never been demonstrated empirically. We have developed an experimental system using yeast for testing genetic models of sexual selection.
Rogers, D., Greig, D.
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Selection and characterization of FcεRI phospho-ITAM specific antibodies
mAbs, 2019 Post-translational modifications, such as the phosphorylation of tyrosines, are often the initiation step for intracellular signaling cascades. Pan-reactive antibodies against modified amino acids (e.g., anti-phosphotyrosine), which are often used to ...
Nileena Velappan +10 more
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Strategies to Screen Anti-AQP4 Antibodies from Yeast Surface Display Libraries
Antibodies, 2022 A rapid and effective method to identify disease-specific antibodies from clinical patients is important for understanding autoimmune diseases and for the development of effective disease therapies.
Aric Huang +11 more
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Yeast Display of Engineered Antibody Domains [PDF]
, 2012 Yeast display is an efficient technology for selection of antibodies and other proteins with high affinity and thermal stability. Here, we describe a method for affinity maturation of engineered antibody domains (eAds) using yeast display. EAd yeast libraries of relatively large size (∼10⁹) were generated and subjected to alternating rounds of magnetic-
Qi, Zhao +2 more
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Construction of Yeast Surface-Displayed cDNA Libraries [PDF]
, 2011 Using yeast display, heterologous protein fragments can be efficiently displayed at high copy levels on the Saccharomyces cerevisiae cell wall. Yeast display can be used to screen large expressed protein libraries for proteins or protein fragments with specific binding properties.
Scott, Bidlingmaier, Bin, Liu
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