Susceptibility of broad reactivity nanobodies to resistance mutations in the S2 domain of SARS-CoV-2 predicted by yeast display deep mutational scanning [PDF]
Frontiers in ImmunologyIntroductionThe rapid evolution of SARS-CoV-2 has led to the erosion of vaccine induced serum neutralization and monoclonal antibody efficacy. As such, interest is inevitably moving towards more conserved regions of the SARS-CoV-2 spike protein like the ...
Christina R. Ball +3 more
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Development of Recombinant Antibody by Yeast Surface Display Technology
Current Research in Pharmacology and Drug DiscoveryRecombinant antibodies have emerged as powerful tools in various fields, including therapeutics, diagnostics, and research applications. The selection of high-affinity antibodies with desired specificity is a crucial step in the development of ...
Mujahed I. Mustafa +2 more
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Yeast surface display for protein engineering and characterization
Current Opinion in Structural Biology, 2007 Yeast surface display is being employed to engineer desirable properties into proteins for a broad variety of applications. Labeling with soluble ligands enables rapid and quantitative analysis of yeast-displayed libraries by flow cytometry, while cell-surface selections allow screening of libraries with insoluble or even as-yet-uncharacterized binding
Gai, S Annie, Wittrup, K Dane
openaire +4 more sources
Efficient yeast surface-display of novel complex synthetic cellulosomes [PDF]
Microbial Cell Factories, 2018 Background The self-assembly of cellulosomes on the surface of yeast is a promising strategy for consolidated bioprocessing to convert cellulose into ethanol in one step.
Hongting Tang +6 more
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Screening of Yeast Display Libraries of Enzymatically Treated Peptides to Discover Macrocyclic Peptide Ligands. [PDF]
Int J Mol Sci, 2021 Bowen J +5 more
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The coordination of cell growth during fission yeast mating requires Ras1-GTP hydrolysis [PDF]
, 2013 The spatial and temporal control of polarity is fundamental to the survival of all organisms. Cells define their polarity using highly conserved mechanisms that frequently rely upon the action of small GTPases, such as Ras and Cdc42.
A Merla +58 more
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Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens
Frontiers in Immunology, 2022 Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments.
Matheus Oliveira de Souza +20 more
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Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients
Frontiers in Immunology, 2021 The re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can induce protective antibody responses.
Ahmed S. Fahad +21 more
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A Generic Strategy to Generate Bifunctional Two-in-One Antibodies by Chicken Immunization
Frontiers in Immunology, 2022 Various formats of bispecific antibodies exist, among them Two-in-One antibodies in which each Fab arm can bind to two different antigens. Their IgG-like architecture accounts for low immunogenicity and also circumvents laborious engineering and ...
Julia Harwardt +10 more
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Cell Reports, 2021 Summary: Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a ...
Bailey B. Banach +33 more
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