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Development of Recombinant Antibody by Yeast Surface Display Technology
Current Research in Pharmacology and Drug DiscoveryRecombinant antibodies have emerged as powerful tools in various fields, including therapeutics, diagnostics, and research applications. The selection of high-affinity antibodies with desired specificity is a crucial step in the development of ...
Mujahed Mustafa
exaly +5 more sources
Biosensors, 2021 C-reactive protein (CRP) is an inflammation biomarker that should be quantified accurately during infections and healing processes. Nanobodies are good candidates to replace conventional antibodies in immunodiagnostics due to their inexpensive production,
Ario De Marco +2 more
exaly +3 more sources
626 Improving the yeast transformation efficiency for yeast display in antibody development
Journal for ImmunoTherapy of Cancer, 2020 Background In the therapeutic antibody development process, the yeast display technology which expresses a large library of antibodies is very useful for increasing the affinity of a lead antibody. Ideally, a yeast library should exceed the size of 10E10 to 10E11 to get close to the real affinity maturation process.
Jian Chen, George Sun
doaj +2 more sources
Mapping monoclonal anti-SARS-CoV-2 antibody repertoires against diverse coronavirus antigens
Frontiers in Immunology, 2022 Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have emerged continuously, challenging the effectiveness of vaccines, diagnostics, and treatments.
Matheus Oliveira de Souza +20 more
doaj +1 more source
Functional Profiling of Antibody Immune Repertoires in Convalescent Zika Virus Disease Patients
Frontiers in Immunology, 2021 The re-emergence of Zika virus (ZIKV) caused widespread infections that were linked to Guillain-Barré syndrome in adults and congenital malformation in fetuses, and epidemiological data suggest that ZIKV infection can induce protective antibody responses.
Ahmed S. Fahad +21 more
doaj +1 more source
A Generic Strategy to Generate Bifunctional Two-in-One Antibodies by Chicken Immunization
Frontiers in Immunology, 2022 Various formats of bispecific antibodies exist, among them Two-in-One antibodies in which each Fab arm can bind to two different antigens. Their IgG-like architecture accounts for low immunogenicity and also circumvents laborious engineering and ...
Julia Harwardt +10 more
doaj +1 more source
Binding pocket stabilization by high-throughput screening of yeast display libraries
Frontiers in Molecular Biosciences, 2022 Protein dynamics have a great influence on the binding pockets of some therapeutic targets. Flexible protein binding sites can result in transient binding pocket formation which might have a negative impact on drug screening efforts.
Jorge A. Lerma Romero +8 more
doaj +1 more source
Cell Reports, 2021 Summary: Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a ...
Bailey B. Banach +33 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2020 Affinity chromatography provides an excellent platform for protein purification, which is a key step in the large scale downstream processing of therapeutic monoclonal antibodies (Mabs).
Steffen C. Hinz +10 more
doaj +1 more source
A switchable yeast display/secretion system [PDF]
Protein Engineering Design and Selection, 2015 Display technologies such as yeast and phage display offer powerful alternatives to traditional immunization-based antibody discovery, but require conversion of displayed proteins into soluble form prior to downstream characterization. Here we utilize amber suppression to implement a yeast-based switchable display/secretion system that enables the ...
James A, Van Deventer +4 more
openaire +2 more sources