Results 181 to 190 of about 27,806 (225)

RAMA casein zymography: Time‐saving and highly sensitive casein zymography for MMP7 and trypsin

ELECTROPHORESIS, 2016
To detect metalloproteinase‐7 (MMP7), zymography is conducted using a casein substrate and conventional CBB stain. It has disadvantages because it is time consuming and has low sensitivity. Previously, a sensitive method to detect MMP7 up to 30 pg was reported, however it required special substrates and complicated handlings.
Hidetaro, Yasumitsu, Yasuhiro, Ozeki, Robert A, Kanaly   +2 more
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Aspartic Protease Zymography Case Study: Detection of Fungal Acid Proteases by Zymography

2017
This chapter describes a method for the production and characterization of fungal acid proteases. Protease production is induced by growth on BSA media over a pH gradient and protein levels are monitored over time with the Bradford assay. Once protein is depleted, the media is purified and proteases are characterized by gelatin zymography using ...
Gavin, Kernaghan, Michael, Mayerhofer
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Analysis of Murein Hydrolases and Proteases Through Zymography

2021
Zymography has been used to analyze enzymatic activity and processing of enzymes for many years. We have used bacterial cells copolymerized into the acrylamide gel to analyze specific activity of murein hydrolases of interest. In addition, this method has been widely used to examine and distinguish protease activities using different substrates.
Nichole A, Seawell, Jeffrey L, Bose
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Loading-Controlled Zymography

Gelatin zymography is a widely used technique to study matrix metalloproteinases (MMP)-2 and -9, characterized by its simplicity, low cost, and high sensitivity. Zymography can detect picogram quantities of MMPs, which is 100-1000-fold more sensitive than immunoblot.
Aleksandra, Franczak, Paul, Jurasz
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Reverse Zymography: Overview and Pitfalls

2017
Reverse zymography is a technique by which protease inhibitor(s) in a sample could be electrophoretically separated in a substrate-impregnated acrylamide gel and their relative abundance could be semi-quantified. The gel after electrophoresis is incubated with a protease when the impregnated substrate and all other proteins of the sample are degraded ...
Kanika, Sharma, Debasish, Bhattacharyya
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Use of Zymography in Trypanosomiasis Studies

2017
Zymography assay is a semiquantitative technique, very sensitive, and commonly used to determine metalloproteinase levels in different types of biological samples, including tissues, cells, and extracts of protein. Samples containing metalloproteinases are loaded onto a polyacrylamide gel containing sodium dodecyl sulphate (SDS) and a specific ...
Jéssyka Fernanda Santiago, Monte, Cláudia Jassica Gonçalves, Moreno, Joana Patrícia Molato Figueiredo Lopes, Monteiro, Hugo Alexandre, de Oliveira Rocha, Aline Rimoldi, Ribeiro, Marcelo Sousa, Silva   +5 more
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Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography

2017
To detect serine protease activity by zymography, casein and CBB stain have been used as a substrate and a detection procedure, respectively. Casein zymography has been using substrate concentration at 1 mg/mL and employing conventional CBB stain. Although ordinary casein zymography provides reproducible results, it has several disadvantages including ...
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Cysteine Protease Zymography: Brief Review

2017
Cysteine proteases play multiple roles in basically all aspects of physiology and development. In plants, they are involved in growth and development and in accumulation and mobilization of storage proteins. Furthermore, they are engaged in signalling pathways and in the response to biotic and abiotic stresses.
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Zymography: Unveiling Matrix Metalloproteinase Dynamics

Zymography is a laboratory technique used to study the activity of enzymes, particularly proteases and glycosidases, in biological samples. It involves the separation of enzymatic proteins by gel electrophoresis under non-denaturing conditions, followed by their renaturation and incubation with specific substrates that mimic natural substrates.
Qinghe, Meng, Adam, Novak, Erin, Rayhill
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Zymography with Caseogram Prints: Quantification of Pepsinogen

Analytical Biochemistry, 2001
In caseogram prints, a type of zymogram which is designed for the detection of acid proteases, enzyme activity is detected in an overlay gel of agarose containing skim milk. The use of this technique for protease quantification was investigated in this study using pepsinogen as an example protease.
O, Till, E, Baumann, W, Linss
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