Results 91 to 100 of about 1,809,550 (296)

Cryo‐EM Structure Guided Engineering of Botulinum Neurotoxin A With Advanced Receptor Binding Affinity and Therapeutical Benefits

Advanced Science, EarlyView.
The butterfly unfolded wing in an open form structure of botulinum neurotoxin type A (BoNT/A) at physiological‐state was confirmed at 2.85 Å resolution by cryo‐electron microscopy (cryo‐EM). Structure‐guided protein engineering significantly enhanced the receptor‐binding affinity, therapeutic efficacy, and safety of the engineered toxin variants ...
Wenrui Wang, Zhaxi Zerang, Linjin You, Ziye Liu, Rong Nie, Fuwei Qi, Fenfen Gao, Chengmu Zhao, Wantong Ma, Jinghan He, Xiaoru Wang, Shanquan Wu, Bo Liu, Xinyao Liu, Dongsheng Lei, Dejuan Zhi, Dongsheng Wang   +16 more
wiley   +1 more source

Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis

Advanced Science, EarlyView.
This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou, Yongfeng Chen, Longyou Xiao, Wei Qi, Tianshu Du, Qiang Sun, Borui Xue, Qinghe Zhao, Pengfei Xie, Zi Ye, Fei Han, Lingli Guo, Yang Jiao, Ranran Zhang, Hong Jia, Xiao Huang, Jianwei Guo, Peng Wang, Huayi Wang, Yuanrui Wang, Wenjing Chen, Haining Wu, Liumin He, Dawei Zhang   +23 more
wiley   +1 more source

Soil hydrolase activities and kinetic properties as affected by wheat cropping systems of Northeastern China

Plant, Soil and Environment, 2010
Agricultural practices that reduce soil degradation and improve agriculture sustainability are important particularly for dry hilly land of Chaoyang County in the Liaoning Province, North-east China, where cinnamon soils are widely distributed and mainly
Y.L. Zhang, L.J. Chen, C.X. Sun, Z.J. Wu, Z.H. Chen, G.H. Dong   +5 more
doaj   +1 more source

MOESM1 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

, 2016
Additional file 1. Stoichiometric and carbon atom transition model for P. pastoris.
Liu, Ping, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Chu, Ju, Yingping Zhuang, Siliang Zhang   +7 more
openaire   +1 more source

Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing [PDF]

, 2014
BACKGROUND: The use of cold-active enzymes has many advantages, including reduced energy consumption and easy inactivation. The ikaite columns of SW Greenland are permanently cold (4-6°C) and alkaline (above pH 10), and the microorganisms living there ...
Jan Vester, Mikkel Glaring, Peter Stougaard   +2 more
core   +2 more sources

Integration of Spatiotemporal Multi‐Omics in Peach Fruit Unravels a Metabolic Niche and the Genetic Basis of Trichome‐Mediated Stress Adaptation

Advanced Science, EarlyView.
This study constructed the first spatiotemporal multi‐omics map of peach fruit and discovered a key candidate gene that synergistically regulates trichome development and drought tolerance through the jasmonic acid signaling pathway, providing insights into the coupling mechanism between development and stress resistance.
Zhixin Liu, Ke Cao, Liping Guan, Aizhi Qin, Yong Li, Zihao Zhao, Yinpeng Zhang, Yaping Zhou, Lirong Wang, Xuwu Sun   +9 more
wiley   +1 more source

Newborn Screening for Lysosomal Storage Disorders in Belgium

Journal of Inborn Errors of Metabolism and Screening, 2017
Lysosomal storage disorders (LSDs) are a group of metabolic disorders with various clinical presentations, which complicate diagnosis. A pilot study was performed to test the appropriateness and effectiveness of the newborn screening method for Pompe ...
Francois Eyskens MD, PhD, Sylvie Devos PhD   +1 more
doaj   +1 more source

MOESM4 of Combined 13C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

, 2016
Additional file 4. Trends of the MID of intracellular free amino acids during 13C labeling experiments on Glc and on Glc/Glu.
Liu, Ping, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Chu, Ju, Yingping Zhuang, Siliang Zhang   +7 more
openaire   +1 more source

A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells [PDF]

FEMS Microbiology Letters, 1995
A procedure is described which allows the rapid permeabilization of yeast cells, Schizosaccharomyces pombe and Saccharomyces cerevisiae, for quantitative in situ assays of beta-galactosidase activity. Yeast cells are permeabilized by incubation in buffer containing 0.2% of the detergent sodium lauroyl sarcosinate without any need for washing or ...
openaire   +2 more sources

Anderson-Fabry’s Disease: A Rare but Treatable Case of Fever of Unknown Origin [PDF]

, 2017
Anderson-Fabry’s disease (AFD) is a rare, X-linked lysosomal storage disorder caused by the complete deficiency or attenuated activity of the enzyme α-galactosidase A, leading to progressive systemic intracellular accumulation of glycosphingolipids and ...
Antuzzi, Daniela, Crea, Filippo, Graziani, Francesca, Iaconelli, Antonio, Manna, Raffaele, Morrone, Amelia, Ruggio, Aureliano, Verrecchia, Elena   +7 more
core   +3 more sources