The zinc cluster proteins Upc2 and Ecm22 promote filamentation in Saccharomyces cerevisiae by sterol biosynthesis-dependent and -independent pathways. [PDF]
, 2015 The transition between a unicellular yeast form to multicellular filaments is crucial for budding yeast foraging and the pathogenesis of many fungal pathogens such as Candida albicans.
Höfken, T, Woods, K
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Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria. [PDF]
Proceedings of the National Academy of Sciences, 1984 The gene coding for the yeast mitochondrial F1-ATPase beta subunit (ATP2) has been fused to the Escherichia coli lacZ gene. The chimeric ATP2-lacZ gene codes for a hybrid protein consisting of some 350 amino acids of the F1-ATPase beta subunit at its amino terminus and a large enzymatically active portion of the lacZ gene product, beta-galactosidase ...
Douglas, Michael G. +2 more
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Advanced Science, EarlyView.D‐galactose (D‐gal) induced inner ear hair cell senescence by inhibiting TFEB transcription. RONIN/HCF1 promotes TFEB transcription to prevent cochlear HCs from D‐gal‐induced senescence through autophagy activation. Abstract Age‐related hearing loss is characterized by senescent inner ear hair cells (HCs) and reduced autophagy.
Yongjie Wei +18 more
wiley +1 more source
Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface [PDF]
, 2002 BACKGROUND: Secretion of anti-host proteins by Yersinia pestis via a type III mechanism is not constitutive. The process is tightly regulated and secretion occurs only after an appropriate signal is received.
Matson, Jyl S, Nilles, Matthew L
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Onecut-dependent Nkx6.2 transcription factor expression is required for proper formation and activity of spinal locomotor circuits. [PDF]
, 2020 In the developing spinal cord, Onecut transcription factors control the diversification of motor neurons into distinct neuronal subsets by ensuring the maintenance of Isl1 expression during differentiation.
A Espana +36 more
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Improved methods for the detection of β-galactosidase activity in colonies ofEscherichia coliusing a new chromogenic substrate: VBzTM-gal (2-(2-(4-(β-d-galactopyranosyloxy)-3-methoxyphenyl)-vinyl)-3-methylbenzothiazolium toluene-4-sulphonate) [PDF]
FEMS Microbiology Letters, 1991 The use of a new substrate 2-(2-(4-(beta-D-galactopyranosyloxy)-3- methoxyphenyl)-vinyl)-3-methylbenzothiazolium toluene-4-sulphonate (VB-zTM-gal) is described for the detection of beta-galactosidase activity in colonies of wild type and mutant strains of Escherichia coli.
A.C. Richardson +5 more
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Advanced Science, EarlyView.This study reveals histone lactylation as a metabolic‐epigenetic regulator countering cellular senescence and skeletal muscle aging. Reduced lactylation impairs cell cycle, DNA repair and proteostasis pathways, while exercise restores glycolytic activity and lactylation levels, preserving muscle function.
Fanju Meng +16 more
wiley +1 more source
Angewandte Chemie, EarlyView.Sialyl glycosides containing 4‐N‐derivatized sialic acids synthesized by a highly efficient one‐pot two‐enzyme (OP2E) chemoenzymatic sialylation strategy have been shown to be versatile probes. They can resist sialidase cleavage, be selective substrates by influenza sialidases, or be nanomolar substrate analog‐based inhibitors and affinity ligands ...
Yue Yuan +6 more
wiley +2 more sources
Priming of a β-Galactosidase (β-GAL)-Specific Type 1 Response in BALB/c Mice Infected with β-GAL-TransfectedLeishmania major [PDF]
Infection and Immunity, 2000 ABSTRACTTo determine whether an ongoing response toLeishmania majorwould affect the response to a non-cross-reacting, non-leishmanial antigen, susceptible BALB/c mice and resistant C3H mice were infected withL. majorparasites expressingEscherichia coliβ-galactosidase (β-GAL); this parasite was designatedL. major-βGAL.
Hrishekesh R. Chakkalath +6 more
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Inhibition of Ferroptosis Delays Aging and Extends Healthspan Across Multiple Species
Advanced Science, EarlyView.This study identifies ferroptosis as a key driver of cellular senescence, with its inhibition delaying aging and extending healthspan across species. During senescence, ferroptosis worsens, increasing oxidative stress and lipid peroxidation, which accelerate aging.
Hai‐Jun Fu +15 more
wiley +1 more source