Results 41 to 50 of about 978,491 (280)

Isolation and Identification of Beta-galactosidase Producing Yeasts From Some Dairy Products [PDF]

Fayoum Journal of Agricultural Research and Development
Twenty-two β-galactosidase-producing yeast isolates were isolated from local dairy products. The purified isolates were evaluated for their β-galactosidase activity to identify the most productive isolates for subsequent investigations. The isolates were
Magda Abdalla, Yaser Hafez, Wedad EL-Kholy, Abdel Moneim Hassan, Hemmat Mahmoud   +4 more
doaj   +1 more source

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources

Effect of yeast extract supplementation on β-galactosidase activity of Lactobacillus delbrueckii subsp. bulgaricus 11842 grown in whey.

Czech Journal of Food Sciences, 2001
Lactobacillus delbrueckii subsp. bulgaricus 11842 (LB 11842) was grown in reconstituted sweet whey supplemented with 0 to 1% yeast extract. The β-galactosidase activity was determined from the hydrolysis of o-nitrophenyl- β-D-galactopyranoside (ONPG) by ...
D. Bury, J. Geciova, P. Jelen
doaj   +1 more source

Rational design of a lipophilic β-galactosidase-responsive near-infrared probe for in vivo imaging of cellular senescence. [PDF]

Smart Mol
We developed a lipophilic near‑infrared probe (DCIP‑AcGal) that is specifically activated by senescence‑associated β‑galactosidase. After intravenous injection, it lights up therapy‑induced tumor senescence in living mice. Its optimized lipophilicity also enables blood‑brain barrier crossing to detect natural brain senescence ex vivo. This probe offers
Huang J, Chu F, Feng B, Chen L, Cen P, Wang J, Qu Y, Hong L, Wu F, Tian H, Fang Y, Chen Y, Tian M, Zeng W, Zhang H, Zhong Y.   +15 more
europepmc   +2 more sources

Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects [PDF]

FEMS Microbiology Ecology, 2005
Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. beta-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon.
Tao, H, Priebe, MG, Vonk, RJ, Welling, GW   +3 more
openaire   +3 more sources

Site-directed mutation of β-galactosidase from Streptococcus thermophilus for galactooligosaccharide-enriched yogurt making

Journal of Dairy Science, 2022
: β-Galactosidase is one of the most important enzymes used in dairy processing. It converts lactose into glucose and galactose, and also catalyzes galactose to form galactooligosaccharides (GOS), so-called prebiotics.
J.C. Zhao, Y.L. Mu, X.Y. Gu, X.N. Xu, T.T. Guo, J. Kong   +5 more
doaj   +1 more source

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

Modeling of the simultaneous hydrolysis-ultrafiltration of whey permeate by a thermostable β-galactosidase from Aspergillus niger

, 2005
A wild type strain of Aspergillus niger, denoted as BTL, produced elevated levels of β-galactosidase when grown in a low cost medium that contained wheat bran as the sole carbon and energy source.
Mamma, D, Christakopoulos, P, Katsifas, Efstathios,, Christakopoulos, Paul, Karagouni, Amalia D.,, Mamma, Diomi, Hatzinikolaou, DG, Karagouni, AD, Katsifas, E, Kekos, Dimitris, Kekos, D, Hatzinikolaou, Dimitris G.,   +11 more
core   +1 more source

A β-Galactosidase-Based Bacterial Two-Hybrid System To Assess Protein−Protein Interactions in the Correct Cellular Environment

, 2007
The vast majority of proteins functions in complex with one or more of the same or other proteins, indicating that protein−protein interactions play crucial roles in biology.
Bart Devreese (146471), Lina De Smet (301198), Bjorn Vergauwen (72280), Jozef J. Van Beeumen (2948115), Jimmy Borloo (1916347)   +4 more
core   +2 more sources

ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS

Química Nova, 2023
The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size ...
Shakeel Ahmed Ansari, Rukhsana Satar, Nadeem Ikram, Muhammad Awais   +3 more
doaj   +1 more source