Results 51 to 60 of about 2,747,637 (331)

Insertion of a 27 amino acid viral peptide in different zones ofEscherichia coliβ-galactosidase: Effects on the enzyme activity [PDF]

FEMS Microbiology Letters, 1994
Seven internal, putatively exposed regions of Escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. Small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacZ gene to generate unique BamHI restriction sites. By using these mutant
A, Benito, A, Villaverde
openaire   +2 more sources

Isolation and Identification of Beta-galactosidase Producing Yeasts From Some Dairy Products [PDF]

Fayoum Journal of Agricultural Research and Development
Twenty-two β-galactosidase-producing yeast isolates were isolated from local dairy products. The purified isolates were evaluated for their β-galactosidase activity to identify the most productive isolates for subsequent investigations. The isolates were
Magda Abdalla, Yaser Hafez, Wedad EL-Kholy, Abdel Moneim Hassan, Hemmat Mahmoud   +4 more
doaj   +1 more source

Cloning and characterization of a novel α-galactosidase fromBifidobacterium breve203 capable of synthesizing Gal-α-1,4 linkage [PDF]

FEMS Microbiology Letters, 2008
A novel alpha-galactosidase gene (aga2) was cloned from Bifidobacterium breve 203. It contained an ORF of 2226-bp nucleotides encoding 741 amino acids with a calculated molecular mass of 81.5 kDa. The recombinant enzyme Aga2 was heterogeneously expressed, purified and characterized. Regarding substrate specificity for hydrolysis, Aga2 was highly active
Han, Zhao, Lili, Lu, Min, Xiao, Qinpeng, Wang, Yu, Lu, Chunhui, Liu, Peng, Wang, Hidehiko, Kumagai, Kenji, Yamamoto   +8 more
openaire   +2 more sources

Effect of yeast extract supplementation on β-galactosidase activity of Lactobacillus delbrueckii subsp. bulgaricus 11842 grown in whey.

Czech Journal of Food Sciences, 2001
Lactobacillus delbrueckii subsp. bulgaricus 11842 (LB 11842) was grown in reconstituted sweet whey supplemented with 0 to 1% yeast extract. The β-galactosidase activity was determined from the hydrolysis of o-nitrophenyl- β-D-galactopyranoside (ONPG) by ...
D. Bury, J. Geciova, P. Jelen
doaj   +1 more source

Identification of bacteria with β-galactosidase activity in faeces from lactase non-persistent subjects [PDF]

FEMS Microbiology Ecology, 2005
Previous studies suggest that, besides the maldigestion of lactose in the small intestine, the colonic processing of lactose might play a role in lactose intolerance. beta-Galactosidase is the bacterial enzyme which catalyzes the first step of lactose fermentation in the colon.
Tao, H, Priebe, MG, Vonk, RJ, Welling, GW   +3 more
openaire   +3 more sources

Design strategies and biological applications of β-galactosidase fluorescent sensor in ovarian cancer research and beyond

RSC Advances
Beta-galactosidase (β-galactosidase), a lysosomal hydrolytic enzyme, plays a critical role in the catalytic hydrolysis of glycosidic bonds, leading to the conversion of lactose into galactose.
Liangliang Li, Feifei Jia, Yunxiu Li, Yan Peng   +3 more
semanticscholar   +1 more source

α-Galactosidase A fromPseudomonas fluorescenssubsp.cellulosa: cloning, high level expression and its role in galactomannan hydrolysis [PDF]

FEMS Microbiology Letters, 2000
A library of Pseudomonas fluorescens subsp. cellulosa genomic DNA, constructed in lambda ZAPII, was screened for alpha-D-galactosidase activity. The DNA inserts from six galactosidase-positive clones were rescued into plasmids. Restriction digestion and Southern analysis revealed that each of the plasmids contained a common DNA sequence.
J R, Halstead, M P, Fransen, R Y, Eberhart, A J, Park, H J, Gilbert, G P, Hazlewood   +5 more
openaire   +2 more sources

ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS

Química Nova, 2023
The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size ...
Shakeel Ahmed Ansari, Rukhsana Satar, Nadeem Ikram, Muhammad Awais   +3 more
doaj   +1 more source

Supplemental material for Detection of Bacteria in Water with β-Galactosidase-Coated Magnetic Nanoparticles

, 2018
Supplemental material for Detection of Bacteria in Water with β-Galactosidase-Coated Magnetic Nanoparticles by Mingyue Cui, Hao Chang, Yang Zhong, Min Wang, Tianze Wu, Xiao Hu, Zhichuan J.
Hao Chang (801491), Mingyue Cui (3925478), Zhichuan J. Xu (1553275), Tianze Wu (5213024), Xiao Hu (233579), Chenjie Xu (135659), Min Wang (21070), Yang Zhong (212360)   +7 more
core   +1 more source

Selective synthesis of galactooligosaccharides containing β(1→3) linkages with β-galactosidase from Bifidobacterium bifidum (Saphera).

Journal of Agricultural and Food Chemistry, 2020
The transglycosylation activity of a novel commercial β-galactosidase from Bifidobacterium bifidum (Saphera) was evaluated. The optimal conditions of operation of this enzyme, measured with o-nitrophenyl-β-D-galactopyranoside, were 40 °C and pH around 6 ...
Vera Füreder, Bárbara Rodriguez-Colinas, Fadia V. Cervantes, L. Fernandez-Arrojo, A. Poveda, J. Jiménez-Barbero, Antonio O. Ballesteros, F. Plou   +7 more
semanticscholar   +1 more source