Thermostable α-galactosidase fromThermotoga neapolitana: cloning, sequencing and expression [PDF]
FEMS Microbiology Letters, 1998 A gene encoding a thermostable alpha-galactosidase from the hyperthermophile Thermotoga neapolitana was cloned and sequenced. Sequence analysis showed that the 552-amino acid protein is similar to Escherichia coli Raf type alpha-galactosidase and belongs to Family 36 of the glycosyl hydrolases. Recombinant alpha-galactosidase expressed in E. coli has a
M R, King +3 more
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Compression behavior of the enzyme ß-galactosidase [PDF]
, 2004 This thesis is based on the investigation of the compression behavior of a solid model enzyme. It was the scope of this work to characterize the behavior of the enzyme powder under pressure to gain on the one hand information about the behavior of ...
Kuny, Tanja Tamara
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Genome-Wide Analysis of β-Galactosidases in Xanthomonas campestris pv. campestris 8004
Frontiers in Microbiology, 2018 Bacterial β-galactosidase is involved in lactose metabolism and acts as a prevalent reporter enzyme used in studying the activities of prokaryotic and eukaryotic promoters. Xanthomonas campestris pv.
Huiqi Wang +13 more
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Expression of β-galactosidase inRhizobium japonicum [PDF]
FEMS Microbiology Letters, 1985 The plasmid pGC91.14 was used to introduce via conjugation the Escherichia coli lac operon into fast-growing and slow-growing strains of Rhizobium japonicum. Exconjugants now expressed higher levels of β-galactosidase activity which was still inducible by isopropyl-β-d-thiogalactoside (IPTG).
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Transcriptional analysis of the β-galactosidase gene (pbg) inClostridium perfringens [PDF]
FEMS Microbiology Letters, 1995 The mode of expression of the beta-galactosidase gene (pbg) of Clostridium perfringens was examined. The pbg gene was transcribed on a single 3.7-kb mRNA. The transcript contained a message for ORF54, located upstream of the pbg gene in the chromosome, indicating that ORF54 and the pbg gene comprise one operon (pbg operon). Expression of the pbg operon
T, Kobayashi, T, Shimizu, H, Hayashi
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Thermostable α-galactosidase fromBacillus stearothermophilusNUB3621: cloning, sequencing and characterization [PDF]
FEMS Microbiology Letters, 1999 An alpha-galactosidase gene from the thermophilic bacterium Bacillus stearothermophilus NUB3621 was cloned, sequenced, expressed in Escherichia coli and the recombinant protein was purified. The Bacillus enzyme, designated AgaN, is similar to alpha-galactosidases of family 36 in the classification of glycosyl hydrolases.
O, Fridjonsson +3 more
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Anaerobic expression of thegyrAgene demonstrated by β-galactosidase activity ingyrA::Mu-lacfusion derivatives ofEscherichia coli [PDF]
FEMS Microbiology Letters, 1988 E. coli C600Δ lac was infected with a Mu- lac defective phage. Lysogens resistant to ampicillin (25 µg/ml) and nalidixic acid (20 µg/ml) were unable to grow anaerobically and insensitive to nalidixic acid (150 µg/ml), indicating formation of gyrA ::Mu- lac fusions.
Nobuto Yamamoto, Mary L. Droffner
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Experimental conditions may affect reproducibility of the beta-galactosidase assay [PDF]
FEMS Microbiology Letters, 1992 Several experimental conditions and parameters contributing to the determination of beta-galactosidase activity, as proposed in Miller's assay, were studied. Use of the absorbance correction factor and the nature and concentration of permeabilizing agents were taken into account as different experimental conditions.
GIACOMINI, ALESSIO +4 more
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ARF48 blocks expression of β-galactosidase.
, 2022 ARF48 blocks expression of β-galactosidase.
Mangala Tawde (9561074) +4 more
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Alternative splicing of β-galactosidase mRNA generates the classic lysosomal enzyme and a β-galactosidase-related protein [PDF]
, 1989 We have isolated two cDNAs encoding human lysosomal β-galactosidase, the enzyme deficiency in G(M1)-gangliosidosis and Morquio B syndrome, and a β-galactosidase-related protein. In total RNA from normal fibroblasts a major mRNA of about 2.5 kilobases (kb)
Morreau, H. +5 more
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