Alternative splicing of β-galactosidase mRNA generates the classic lysosomal enzyme and a β-galactosidase-related protein [PDF]
, 1989 We have isolated two cDNAs encoding human lysosomal β-galactosidase, the enzyme deficiency in G(M1)-gangliosidosis and Morquio B syndrome, and a β-galactosidase-related protein. In total RNA from normal fibroblasts a major mRNA of about 2.5 kilobases (kb)
Morreau, H. +5 more
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Effect of tunicamycin on α-galactosidase secretion byAspergillus nidulansand the importance ofN-glycosylation [PDF]
FEMS Microbiology Letters, 1994 Aspergillus nidulans released α-galactosidase into the culture medium during the exponential growth on either lactose or galactose as the only carbon source. This enzyme is a glycoprotein. Its treatment with endoglycosidases produces a reduction in its molecular mass but the resulting enzyme conserved some of their carbohydrate components in addition ...
Santiago RÃos +2 more
openaire +1 more source
Optimization of β-galactosidase production from lactic acid bacteria [PDF]
Hemijska Industrija, 2015 β-galactosidase, commonly known as lactase, represents commercially important enzyme that is prevalently used for lactose hydrolysis in milk and whey. To the date, it has been isolated from various sources.
Carević Milica +6 more
doaj +1 more source
Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, (Walbaum) [PDF]
Asian-Australasian Journal of Animal Sciences, 2014 A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding β-mannanase and α-galactosidase enzymes, solely or in combination.
Nalan Ozgur Yiğit +3 more
doaj +1 more source
A food-grade vector for Streptococcus thermophilus based on the α-complementation of β-galactosidase
Journal of Dairy Science, 2022 : Streptococcus thermophilus is a common yogurt starter that consumes lactose as its primary carbon source. The enzyme β-galactosidase is essential for the lactose metabolism and the growth of this species.
Z.S. Xu +5 more
doaj +1 more source
Porungo cheese whey: a new substrate to produce β-galactosidase [PDF]
Anais da Academia Brasileira de Ciências, 2023 The bioconversion of porungo cheese whey to produce β-galactosidase in batch system was studied. The whey released after curd cutting and precipitation during porungo cheese production was collected in borosilicate flasks.
RAFAELA J.S. COELHO +5 more
doaj +1 more source
An engineered genetic circuit for lactose intolerance alleviation
BMC Biology, 2021 Background Lactose malabsorption occurs in around 68% of the world’s population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
Mingyue Cheng +16 more
doaj +1 more source
Purification and molecular properties of an α-galactosidase synthesized and secreted byAspergillus nidulans [PDF]
FEMS Microbiology Letters, 1993 alpha-Galactosidases from mycelial extract and culture filtrate of Aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. The enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme ...
S, Ríos +3 more
openaire +2 more sources
Yersinia pestis lacZexpresses a β-galactosidase with low enzymatic activity [PDF]
FEMS Microbiology Letters, 2006 Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y.
Alexander G, Bobrov, Robert D, Perry
openaire +2 more sources
Thermostable α-galactosidase fromThermotoga neapolitana: cloning, sequencing and expression [PDF]
FEMS Microbiology Letters, 1998 A gene encoding a thermostable alpha-galactosidase from the hyperthermophile Thermotoga neapolitana was cloned and sequenced. Sequence analysis showed that the 552-amino acid protein is similar to Escherichia coli Raf type alpha-galactosidase and belongs to Family 36 of the glycosyl hydrolases. Recombinant alpha-galactosidase expressed in E. coli has a
M R, King +3 more
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