Optimization of β-galactosidase production from lactic acid bacteria [PDF]
β-galactosidase, commonly known as lactase, represents commercially important enzyme that is prevalently used for lactose hydrolysis in milk and whey. To the date, it has been isolated from various sources.
Carević Milica +6 more
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Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, (Walbaum) [PDF]
A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding β-mannanase and α-galactosidase enzymes, solely or in combination.
Nalan Ozgur Yiğit +3 more
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A food-grade vector for Streptococcus thermophilus based on the α-complementation of β-galactosidase
: Streptococcus thermophilus is a common yogurt starter that consumes lactose as its primary carbon source. The enzyme β-galactosidase is essential for the lactose metabolism and the growth of this species.
Z.S. Xu +5 more
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Porungo cheese whey: a new substrate to produce β-galactosidase [PDF]
The bioconversion of porungo cheese whey to produce β-galactosidase in batch system was studied. The whey released after curd cutting and precipitation during porungo cheese production was collected in borosilicate flasks.
RAFAELA J.S. COELHO +5 more
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An engineered genetic circuit for lactose intolerance alleviation
Background Lactose malabsorption occurs in around 68% of the world’s population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
Mingyue Cheng +16 more
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Thermostable α-galactosidase fromThermotoga neapolitana: cloning, sequencing and expression [PDF]
A gene encoding a thermostable alpha-galactosidase from the hyperthermophile Thermotoga neapolitana was cloned and sequenced. Sequence analysis showed that the 552-amino acid protein is similar to Escherichia coli Raf type alpha-galactosidase and belongs to Family 36 of the glycosyl hydrolases. Recombinant alpha-galactosidase expressed in E. coli has a
M R, King +3 more
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Yersinia pestis lacZexpresses a β-galactosidase with low enzymatic activity [PDF]
Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y.
Alexander G, Bobrov, Robert D, Perry
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Purification and molecular properties of an α-galactosidase synthesized and secreted byAspergillus nidulans [PDF]
alpha-Galactosidases from mycelial extract and culture filtrate of Aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. The enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme ...
S, Ríos +3 more
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Genome-Wide Analysis of β-Galactosidases in Xanthomonas campestris pv. campestris 8004
Bacterial β-galactosidase is involved in lactose metabolism and acts as a prevalent reporter enzyme used in studying the activities of prokaryotic and eukaryotic promoters. Xanthomonas campestris pv.
Huiqi Wang +13 more
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Expression of β-galactosidase inRhizobium japonicum [PDF]
The plasmid pGC91.14 was used to introduce via conjugation the Escherichia coli lac operon into fast-growing and slow-growing strains of Rhizobium japonicum. Exconjugants now expressed higher levels of β-galactosidase activity which was still inducible by isopropyl-β-d-thiogalactoside (IPTG).
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