Enzyme-activatable fluorescent probes for β-galactosidase: from design to biological applications
β-Galactosidase (β-gal), a typical hydrolytic enzyme, is a vital biomarker for cell senescence and primary ovarian cancers. Developing precise and rapid methods to monitor β-gal activity is crucial for early cancer diagnoses and biological research. Over
Yongkang Yao +4 more
semanticscholar +1 more source
Porungo cheese whey: a new substrate to produce β-galactosidase [PDF]
The bioconversion of porungo cheese whey to produce β-galactosidase in batch system was studied. The whey released after curd cutting and precipitation during porungo cheese production was collected in borosilicate flasks.
RAFAELA J.S. COELHO +5 more
doaj +1 more source
Effect of β-Mannanase and α-Galactosidase Supplementation to Soybean Meal Based Diets on Growth, Feed Efficiency and Nutrient Digestibility of Rainbow Trout, (Walbaum) [PDF]
A 12-week feeding trial was conducted with 87 g rainbow trout to evaluate the effects on growth performances, feed efficiency and nutrient digestibility of adding β-mannanase and α-galactosidase enzymes, solely or in combination.
Nalan Ozgur Yiğit +3 more
doaj +1 more source
The transgalactosylation rate catalyzed by the lipid-coated β-d-galactosidase in supercritical fluoroform (scCHF3) can be reversibly controlled by changing temperature or pressure (reflecting polarity changes) without damaging ...
Toshiaki Mori (468701) +3 more
core +3 more sources
An engineered genetic circuit for lactose intolerance alleviation
Background Lactose malabsorption occurs in around 68% of the world’s population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
Mingyue Cheng +16 more
doaj +1 more source
A food-grade vector for Streptococcus thermophilus based on the α-complementation of β-galactosidase
: Streptococcus thermophilus is a common yogurt starter that consumes lactose as its primary carbon source. The enzyme β-galactosidase is essential for the lactose metabolism and the growth of this species.
Z.S. Xu +5 more
doaj +1 more source
β-galactosidase-specific band shifts were not observed in monocots and basal angiosperms samples.
(A) Tomato fruits produced GBGM oligo after AnGH5 digestion, which migrated to GAGM after the following β-galactosidase treatment. M: marker lane (standard oligo saccharides).
Alberto Echevarría-Poza (17692296) +7 more
core +1 more source
Purification and molecular properties of an α-galactosidase synthesized and secreted byAspergillus nidulans [PDF]
alpha-Galactosidases from mycelial extract and culture filtrate of Aspergillus nidulans have been purified to homogeneity and utilised to obtain polyclonal antibodies anti-alpha-galactosidase. The enzymatic characteristics and the cross reactivity of the antibodies suggest that alpha-galactosidases isolated from the two sources were the same enzyme ...
S, Ríos +3 more
openaire +2 more sources
Cellular senescence, a persistent state of cell cycle arrest, accumulates in aged organisms, contributes to tissue dysfunction, and drives age-related phenotypes.
Yu-Sheng Cai +13 more
semanticscholar +1 more source
Yersinia pestis lacZexpresses a β-galactosidase with low enzymatic activity [PDF]
Although very little, if any, beta-galactosidase activity is detected in Yersinia pestis by a standard Miller assay, we found that Y. pestis KIM6+ cells formed blue colonies on plates containing 5-bromo-4-chloro-3-indolyl-beta-D-galactoside (X-gal). Searches of the Y.
Alexander G, Bobrov, Robert D, Perry
openaire +2 more sources

