Results 61 to 70 of about 807,747 (247)
Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
Advanced Science, EarlyView.This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Tetrahedron, 2013 Ministerio de Ciencia e Innovacion; UCM/ Santander; Universidad Nacional de Costa ...
Sandoval, Manuel +4 more
openaire +2 more sources
Condições operacionais na hidrólise enzimática da lactose em reator a membrana [PDF]
, 2007 Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico. Programa de Pós-Graduação em Engenharia de Alimentos.A hidrólise enzimática da lactose presente no soro lácteo é uma das possibilidades mais atrativas para utilização ...
Tremarin, Andréia
core
Advanced Science, EarlyView.This study identifies the HDAC6/GATA4/HtrA1 axis as a critical driver of cellular senescence in the inner ear. GATA4 nuclear translocation, facilitated by HDAC6 downregulation, transcriptionally activates HtrA1, promoting hair cell senescence, SASP, and audio‐vestibular dysfunction in models of Ménière's disease and age‐related audio‐vestibular ...
Na Zhang +16 more
wiley +1 more source
Synthesis of a New Substrate for Detection of lacZ Gene Expression in Live Drosophila Embryos
BioTechniques, 1996 Escherichia coli lacZ, which encodes β-galactosidase, has become a widely used reporter gene to study the developmental regulation of gene expression in a variety of organisms.
Jonathan S. Minden
doaj +1 more source
Marine Drugs, 2023 A novel GH2 (glycoside hydrolase family 2) β-galactosidase from Marinomonas sp. BSi20584 was successfully expressed in E. coli with a stable soluble form.
Dianyi Li +7 more
doaj +1 more source
Activated microglia release β-galactosidase that promotes inflammatory neurodegeneration.
, 2023 Beta (β)-galactosidase is a lysosomal enzyme that removes terminal galactose residues from glycolipids and glycoproteins. It is upregulated in, and used as a marker for, senescent cells.
core +1 more source
Advanced Science, EarlyView.We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel +16 more
wiley +1 more source
, 2017 β-galactosidase is used as a reporter for the quantitative analysis of gene expression. It is also used as a histochemical marker. This introduction briefly reviews the enzymatic reactions catalyzed by β-galactosidase and methods for assaying β ...
Pradeep D. Uchil +2 more
core +1 more source
Self‐Assembling Hybrid Hydrogel Reprograms the Stromal Vascular Fraction to Treat Osteoarthritis
Advanced Science, EarlyView.This study presents a bioinspired injectable hydrogel that enhances the therapeutic potential of stem cell‐rich stromal vascular fraction for treating osteoarthritis. By reprogramming cell behavior through epigenetic modulation, the hydrogel promotes cartilage regeneration and reduces joint damage in a rat model, offering a promising new approach for ...
Waifang Hou +23 more
wiley +1 more source