Results 51 to 60 of about 1,201,054 (248)

Identification of host genetic factors modulating β-lactam resistance in Escherichia coli harbouring plasmid-borne β-lactamase through transposon-sequencing

Emerging Microbes and Infections
Since β-lactam antibiotics are widely used, emergence of bacteria with resistance to them poses a significant threat to society. In particular, acquisition of genes encoding β-lactamase, an enzyme that degrades β-lactam antibiotics, has been a major ...
Hyunhee Kim, Travis Bell, Kihyun Lee, Jeongyun Jeong, James C. A. Bardwell, Changhan Lee   +5 more
doaj   +1 more source

Hinge-shift mechanism as a protein design principle for the evolution of β-lactamases from substrate promiscuity to specificity

Nature Communications, 2021
TEM-1 β-lactamase evolved from ancestral enzymes that degraded a variety of β-lactam antibiotics with moderate efficiency and degrades β-lactam antibiotics with a strong preference for penicillins.
Tushar Modi, Valeria A. Risso, Sergio Martinez-Rodriguez, Jose A. Gavira, Mubark D. Mebrat, Wade D. Van Horn, Jose M. Sanchez-Ruiz, S. Banu Ozkan   +7 more
doaj   +1 more source

High‐Density E. Coli Cultivation Arrays for Combinatorial Drug Testing With Subsequent Lipid Profiling by MALDI‐MS

Advanced Science, EarlyView.
High‐throughput antibiotic cross‐gradient assays on microdroplet arrays. A multichannel printhead together with five pressure regulators forms droplet arrays of up to 20 832 droplets within 25 minutes. Each droplet contains unique concentrations of antibiotics, allowing to moniture a vast spectrum of bacterial repsonses over night. The bacterial growth
M. Breitfeld, C. L. Dietsche, F‐L. Born, I. Onipko, L. Blaase, P. S. Dittrich   +5 more
wiley   +1 more source

Rational Design of a Synthetic Galliphore for the Diagnostic Nuclear Imaging and Treatment of Bacterial Infections

Angewandte Chemie, EarlyView.
KO‐NH2 is a synthetic metallophore that preferentially binds Ga3+ over Fe3+ and is recognized by bacterial transporters. Photocrosslinking identifies CirA and Fiu as key uptake proteins in E. coli. KO‐NH2 enables targeted imaging and treatment of E. coli soft tissue infections.
Phuong Nguyen Tran, Axia Marlin, Edith K. Amason, Marie Huynh, Hannah Carlisle, Eszter Boros   +5 more
wiley   +2 more sources

β-lactam Resistance in Pseudomonas aeruginosa: Current Status, Future Prospects

Pathogens, 2021
Pseudomonas aeruginosa is a major opportunistic pathogen, causing a wide range of acute and chronic infections. β-lactam antibiotics including penicillins, carbapenems, monobactams, and cephalosporins play a key role in the treatment of P.
Karl A. Glen, Iain L. Lamont
doaj   +1 more source

The development of non-β-lactam inhibitors of bacterial transpeptidases [PDF]

β-Lactams, which inhibit bacterial transpeptidases, are currently the most widely used antibacterials globally. The emergence of bacterial resistance, conferred by β- lactamases in particular, which degrade β-lactam antibiotics, poses a perilous threat ...
Suits, Timothy Francis
core   +1 more source

Pyrazole‐Derived Antibacterial Compounds Effectively Treat Methicillin‐Resistant Staphylococcus Aureus Infections by Inhibiting Aspartate Transcarbamoylase

Advanced Science, EarlyView.
This study unveils a novel antibacterial mechanism against MRSA, in which the compound Py‐27 selectively targets and inhibits aspartate transcarbamoylase (ATCase), a key enzyme in pyrimidine synthesis. This inhibition disrupts DNA replication, induces oxidative damage, leading to potent bactericidal activity.
Xiaorong Yang, Xinyuan Cao, Lixia Dai, Feng Yang, Jing Wang, Yudong Ma, Tong Bu, Zile Gong, Xueyan Liu, Changcai Bai, Xiaolou Miao, Xiaofei Shang   +11 more
wiley   +1 more source

β-lactam precision dosing in critically ill children: Current state and knowledge gaps

Frontiers in Pharmacology, 2022
There has been emerging interest in implementing therapeutic drug monitoring and model-informed precision dosing of β-lactam antibiotics in critically ill patients, including children. Despite a position paper endorsed by multiple international societies
Sonya Tang Girdwood, Sonya Tang Girdwood, Sonya Tang Girdwood, Kathryn Pavia, Kelli Paice, H. Rhodes Hambrick, Jennifer Kaplan, Jennifer Kaplan, Alexander A. Vinks, Alexander A. Vinks   +9 more
doaj   +1 more source

Asymmetric Single‐Atom Catalysts Govern Diffusible and Non‐Diffusible Non‐Radical Pathways for Selective Degradation of Antibiotic Resistance Genes

Advanced Science, EarlyView.
Asymmetric single‐atom metal‐nitrogen‐carbon catalysts modulate the non‐radical pathway during peroxymonosulfate (PMS) activation. A fundamental contrast was revealed between diffusible singlet oxygen (1O2) and non‐diffusible electron‐transfer pathways (ETP) in antibiotic resistance gene (ARG) degradation.
Chen Gao, Jun Sun, Liang Zhang, Litao Lin, Zongfeng Wan, Bingcai Pan, Sheng Tang   +6 more
wiley   +1 more source

Reversible inactivation of a peptidoglycan transpeptidase by a β-lactam antibiotic mediated by β-lactam-ring recyclization in the enzyme active site

Scientific Reports, 2017
β-lactam antibiotics act as suicide substrates of transpeptidases responsible for the last cross-linking step of peptidoglycan synthesis in the bacterial cell wall.
Zainab Edoo, Michel Arthur, Jean-Emmanuel Hugonnet   +2 more
doaj   +1 more source