Results 41 to 50 of about 5,351 (154)
Emerging treatment strategies for pulmonary fibrosis in precision medicine [PDF]
Pulmonary fibrosis （PF） is a chronic progressive lung disease that poses a serious health risk. It is mainly characterized by abnormal fibrosis and scarring of lung tissues， resulting in the gradual replacement of alveoli with fibrous tissues and ...
XIA Dong, YANG Huiling
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浙江大学学报. 农业与生命科学版Lily (Lilium spp.) is one of the most representative bulbous flowers. However, the vast majority of lilies have a long juvenile stage, which is a significant bottleneck for the development of the entire production industry. In this study, we analyzed the
郭振峰(GUO Zhenfeng) +7 more
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Effects of 1-MCP on the genes related to wax anabolism in pear peel based on transcriptomics [PDF]
[Objective] According to the investigation and laboratory research of our study group for several years, it has been found that the main pear cultivar Yuluxiang was easy to become greasy in the early stage of shelf life at room temperature, and also in ...
JIA Xiaohui +4 more
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野生动物学报, 2015 根据Gen Bank中发表的犬瘟热病毒(CDV)的基因组序列设计了H和F基因的2对引物,通过RT-PCR扩增出从发病小熊猫分离的CDV的H和F基因,并将其克隆入到18T-simple载体上进行测序。使用Kpn I和Xho I限制性内切酶对重组载体进行双酶切并将目的基因克隆到pcDNA 3.1表达载体上,构建完成后转染293T细胞,转染48 h后,再利用RT-PCR和Western-blot检测目的基因的转录和目的蛋白的表达。结果表明,表达载体经转染后,在293T细胞中,目的基因均能得到转录而且正确表达 ...
张晓明 陈 武 彭仕明
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Screening of Anti-hyperuricemia Peptides from Oat Protein Based on High-throughput Transcriptome Sequencing and Network Pharmacology [PDF]
Initiating from omics, the research aimed to discover and filter oat active peptides effective against hyperuricemia, and to study the operational mechanism of these oat active peptides in treating hyperuricemia.
Fei GAO +5 more
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Transcriptomic analysis of the root system of Platycodon grandiflorum in response to the fallen leaves of Acer truncatum [PDF]
[Objective] The aim of this study was to explore the effect of Acer truncatum fallen leaves in Beijing on the accumulation of medicinal active ingredients of Platycodon grandiflorum and to reveal the molecular mechanism of active ingredient synthesis ...
CHENG Shaoying +5 more
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鉴定AP-4是在非激素依赖型前列腺癌中上调L-plastin表达的转录因子
Zhongshan Daxue xuebao. Yixue kexue ban, 2006 【目的】鉴定AP-4是在非激素依赖型前列腺癌中调控L-plastin表达的转录因子，进一步阐明非激素依赖型前列腺癌的发病机制。【方法】在鉴定L-plastin启动子近3’端-216到1序列片段存在明显的转录活性的基础上，利用TFSEARCH软件分析该片段的序列，寻找可能调控L-plastin表达的非甾体激素类转录因子，并用凝胶滞后实验和超滞后实验证实结合于该片段的转录因子，利用PCR定点突变法构建删除该转录因子结合位点的重组子，并检测切除该片段序列后荧光素酶活性 ...
林天歆 +7 more
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Transcriptomic Analysis of Botryosphaeria dothidea on Kiwifruit during the Early Infection Process [PDF]
In this study, RNA sequencing (RNA-Seq) was employed to analyze differential gene expression during the initial stage of kiwifruit infection by Botryosphaeria dothidea, aiming to clarify the mechanism of kiwifruit soft rot at the level of transcriptional
SONG Jinhuang, TAN Hong, ZHAO Zhibo, LONG Youhua, HU Xiaoping, FAN Rong
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Zhongguo shiyan zhenduanxue, 2017 随着高通量测序、基因芯片等技术的兴起以及生物信息学的应用,大量的基因组转录本被揭示,从此众多的研究成果不断涌现,为揭示基因中的奥秘提供了新思路。研究发现[1],人类93%的基因组DNA可转录成RNA,编码蛋白的基因仅仅为人类基因组的2%[2],而其余98%则为不具有或具有极低蛋白质编码功能的非编码RNA（non-coding RNA,ncRNA）。成百上千个非编码RNAs被转录,并且被认为是具有不同大小、结构和生物功能的功能性RNA ...
孙延文, 印璞, 于庭
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重组pDC316-MCMV／hNIS真核表达质粒的构建及在肿瘤细胞的功能
Zhongshan Daxue xuebao. Yixue kexue ban, 2007 【目的】克隆人的钠／碘同向转运体（hNIS）基因可编码序列，并研究其在非甲状腺肿瘤细胞内的功能表达。【方法】利用逆转录和聚合酶链反应（RT-PCR）从毒性弥漫性甲状腺肿（又称Graves病）病人的甲状腺组织中扩增得到NIS的可编码区基因，并克隆到T载体，测序后亚克隆到腺病毒载体穿梭质粒pDC316载体上，获得重组真核表达质粒载体pDC316-MCMV／hNIS。采用脂质体转染的方法，把pDC316-MCMV／hNIS重组质粒（实验组）或pDC316空质粒（对照组）分别导人到胰腺癌细胞株CAPAN-Ⅱ细胞、
周泉波 +5 more
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