Results 71 to 80 of about 505,000 (387)

PCR primers to amplify 16S rRNA genes from cyanobacteria [PDF]

open access: yesApplied and Environmental Microbiology, 1997
We developed and tested a set of oligonucleotide primers for the specific amplification of 16S rRNA gene segments from cyanobacteria and plastids by PCR. PCR products were recovered from all cultures of cyanobacteria and diatoms that were checked but not from other bacteria and archaea. Gene segments selectively retrieved from cyanobacteria and diatoms
Nübel, U.   +2 more
openaire   +3 more sources

The evaluation of an identification algorithm for Mycobacterium species using the 16S rRNA coding gene and rpoB

open access: yesInternational Journal of Mycobacteriology, 2012
Background: Conventional biochemical tests are the standard for the identification of Mycobacterium species, but molecular identifications are becoming more prevalent.
Yuko Kazumi, Satoshi Mitarai
doaj   +1 more source

16S-ITS-23S rRNA operon segment sequencing provides necessary and sufficient conditions for bacterial species-specific identification

open access: yesИнфекция и иммунитет, 2022
Introduction. Sequencing of the 16S rRNA gene is the predominant method for assessing microbial communities and strain molecular identification. The short reads (2nd generation sequencing)-based technology does not allow analysis beyond the 16S rRNA gene.
O. B. Ogarkov   +6 more
doaj   +1 more source

Characterization of Francisella species isolated from the cooling water of an air conditioning system. [PDF]

open access: yes, 2015
Strains of Francisella spp. were isolated from cooling water from an air conditioning system in Guangzhou, China. These strains are Gram negative, coccobacilli, non-motile, oxidase negative, catalase negative, esterase and lipid esterase positive.
Atwill, Edward   +6 more
core   +3 more sources

An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform

open access: yesMicrobiome, 2014
To take advantage of affordable high-throughput next-generation sequencing technologies to characterize microbial community composition often requires the development of improved methods to overcome technical limitations inherent to the sequencing ...
D. Fadrosh   +6 more
semanticscholar   +1 more source

Two different 16S rRNA genes in a mycobacterial strain [PDF]

open access: yesJournal of Clinical Microbiology, 1996
Sequencing of the gene coding for 16S rRNA (16S rDNA) is a well-established method used to identify bacteria, particularly mycobacteria. Unique sequences allow identification of a particular genus and species. If more than one 16S rDNA is present on one mycobacterial genome, their sequences are assumed to be strictly or almost identical.
Bescher Ninet, Béatrice Alice   +7 more
openaire   +3 more sources

Substrate-specific clades of active marine methylotrophs associated with a phytoplankton bloom in a temperate coastal environment [PDF]

open access: yes, 2008
Marine microorganisms that consume one-carbon (C1) compounds are poorly described, despite their impact on global climate via an influence on aquatic and atmospheric chemistry.
Boden, Rich   +4 more
core   +2 more sources

Genetic Environment of 16S rRNA Methylase Gene rmtD [PDF]

open access: yesAntimicrobial Agents and Chemotherapy, 2008
ABSTRACT The genetic environment of the 16S rRNA methylase gene rmtD was investigated. rmtD was flanked by a novel IS CR motif located downstream of class I integron In 163 in the original Pseudomonas aeruginosa
Doi, Y.   +2 more
openaire   +3 more sources

High-throughput amplicon sequencing of the full-length 16S rRNA gene with single-nucleotide resolution

open access: yesbioRxiv, 2018
Targeted PCR amplification and high-throughput sequencing (amplicon sequencing) of 16S rRNA gene fragments is widely used to profile microbial communities.
B. Callahan   +7 more
semanticscholar   +1 more source

Taxonomic and Characterization Methods of Streptomyces: A Review

open access: yesProgress in Microbes and Molecular Biology, 2018
The 16S ribosomal RNA gene is the gold standard for taxonomic identification and phylogenetic study of most of the bacteria. However, the resolution of 16S rRNA gene is found to be insufficient to distinguish closely related Streptomyces species within ...
Jodi Woan-Fei Law   +4 more
doaj   +1 more source

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