Results 21 to 30 of about 12,744 (156)

Regulation of cholesterol biosynthesis and esterification by 25-hydroxycholesterol in a macrophage-like cell line: uncoupling by progesterone.

Journal of Lipid Research, 1984
The coordinated control of cholesterol biosynthesis and esterification by 25-hydroxycholesterol was studied in the macrophage-like cell line P388D1. Since 25-hydroxycholesterol rapidly stimulated incorporation of [3H]oleate into the cholesteryl ester ...
S C Miller, G Melnykovych
doaj   +1 more source

Se doped ZnO nanoparticles with improved catalytic activity in degradation of Cholesterol [PDF]

Journal of Water and Environmental Nanotechnology, 2018
Pristine and Se doped ZnO nanoparticles (NPs) were successfully synthesized by thermo-mechanical method and their structural, morphological and optical properties are characterized.
Bhavani Nenavathu, Aarti Sharma, Raj Kumar Dutta   +2 more
doaj   +1 more source

Marked accumulation of 27-hydroxycholesterol in SPG5 patients with hereditary spastic paresis

Journal of Lipid Research, 2010
Patients with a recessively inherited “pure” hereditary spastic paresis (SPG5) have mutations in the gene coding for the oxysterol 7 α hydroxylase (CYP7B1).
Rebecca Schüle, Teepu Siddique, Han-Xiang Deng, Yi Yang, Sandra Donkervoort, Magnus Hansson, Ricardo E. Madrid, Nailah Siddique, Ludger Schöls, Ingemar Björkhem   +9 more
doaj   +1 more source

In vivo formation of 25-hydroxycholesterol from endogenous cholesterol after a single meal, dietary cholesterol challenge.

Journal of Lipid Research, 1994
The role of oxysterols as regulatory molecules in the suppression of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase activity was investigated in the intact rat in response to an acute dietary cholesterol challenge.
K A Johnson, C J Morrow, G D Knight, T J Scallen   +3 more
doaj   +1 more source

25-Hydroxycholesterol stimulates sphingomyelin synthesis in Chinese hamster ovary cells.

Journal of Lipid Research, 1995
In several experimental and pathological conditions the cellular concentrations of cholesterol and sphingomyelin (SM) change coordinately. In an effort to identify factors mediating co-regulation, a class of suppressors of cholesterol synthesis ...
N D Ridgway
doaj   +1 more source

Highly automated nano-LC/MS-based approach for thousand cell-scale quantification of side chain-hydroxylated oxysterols[S]

Journal of Lipid Research, 2014
Iso-octyl chain-hydroxylated oxysterols were determined in attomoles per 10,000 cells concentrations in 10,000–80,000 cultured pancreatic adenocarcinoma cells, using a sensitive, highly automated nano-LC-ESI-MS-based method.
Hanne Roberg-Larsen, Kaja Lund, Tore Vehus, Nina Solberg, Caroline Vesterdal, Dorna Misaghian, Petter Angell Olsen, Stefan Krauss, Steven Ray Wilson, Elsa Lundanes   +9 more
doaj   +1 more source

Quantification and Localisation of New Brain Lipid Synthesis Using Deuterium Oxide and High Resolution Mass Spectrometry

Angewandte Chemie, EarlyView.
Liquid chromatography tandem mass spectrometry and mass spectrometry imaging were used in combination with deuterium oxide administration to quantify and localise newly synthesised myelin lipids in the mouse brain. This methodology, used here to show sites of myelin repair, enables the measurement of dynamic lipid synthesis in living systems and ...
Catherine Zhang, Jesse A. Michael, Jonathan D. Teo, Huitong Song, Mika T. Westerhausen, Shadrack M. Mutuku, Shane R. Ellis, Anthony S. Don   +7 more
wiley   +2 more sources

Movement of 25-hydroxycholesterol from the plasma membrane to the rough endoplasmic reticulum in cultured hepatoma cells.

Journal of Lipid Research, 1995
Oxysterols serve as both substrates and signal molecules in the cholesterol-utilizing pathways of mammalian cells. Their distribution and movement within these cells, however, have not been well characterized; therefore we have undertaken such an ...
Y Lange, J Ye, F Strebel
doaj   +1 more source

Oxygenation of desmosterol and cholesterol in cell cultures.

Journal of Lipid Research, 1990
In order to determine whether hydration of the delta 24 bond of desmosterol contributes to the formation of the regulatory oxysterol, 25-hydroxycholesterol, [3H]desmosterol was incubated with two cultured cell lines and the labeled products were analyzed.
SE Saucier, AA Kandutsch, AK Gayen, JA Nelson, TA Spencer   +4 more
doaj   +1 more source

Simultaneous determination of oxysterols, cholesterol and 25-hydroxy-vitamin D3 in human plasma by LC-UV-MS. [PDF]

PLoS ONE, 2015
Oxysterols are promising biomarkers of neurodegenerative diseases that are linked with cholesterol and vitamin D metabolism. There is an unmet need for methods capable of sensitive, and simultaneous quantitation of multiple oxysterols, vitamin D and ...
Rohini Narayanaswamy, Vignesh Iyer, Prachi Khare, Mary Lou Bodziak, Darlene Badgett, Robert Zivadinov, Bianca Weinstock-Guttman, Todd C Rideout, Murali Ramanathan, Richard W Browne   +9 more
doaj   +1 more source