Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions [PDF]
Plant Methods, 2019 Background The growing field of plant molecular farming relies on expression vectors that allow high yields of recombinant proteins to be produced through transient gene expression.
Hadrien Peyret +2 more
doaj +3 more sources
Dom34 rescues ribosomes in 3' untranslated regions. [PDF]
Cell, 2014 Ribosomes that stall before completing peptide synthesis must be recycled and returned to the cytoplasmic pool. The protein Dom34 and cofactors Hbs1 and Rli1 can dissociate stalled ribosomes in vitro, but the identity of targets in the cell is unknown. Here, we extend ribosome profiling methodology to reveal a high-resolution molecular characterization
Guydosh NR, Green R.
europepmc +5 more sources
5’ and 3’ untranslated regions strongly enhance performance of geminiviral replicons in Nicotiana benthamiana leaves [PDF]
Frontiers in Plant Science, 2016 We previously reported a recombinant protein production system based on a geminivirus replicon that yields high levels of vaccine antigens and monoclonal antibodies in plants.
Andrew G. Diamos +2 more
doaj +3 more sources
Highly accessible AU-rich regions in 3' untranslated regions are hotspots for binding of regulatory factors. [PDF]
PLoS Computational Biology, 2017 Post-transcriptional regulation is regarded as one of the major processes involved in the regulation of gene expression. It is mainly performed by RNA binding proteins and microRNAs, which target RNAs and typically affect their stability.
Mireya Plass +2 more
doaj +3 more sources
Structures and Functions of the 3′ Untranslated Regions of Positive-Sense Single-Stranded RNA Viruses Infecting Humans and Animals [PDF]
Frontiers in Cellular and Infection Microbiology, 2020 The 3′ untranslated region (3′ UTR) of positive-sense single-stranded RNA [ssRNA(+)] viruses is highly structured. Multiple elements in the region interact with other nucleotides and proteins of viral and cellular origin to regulate various aspects of ...
Yuanzhi Liu +66 more
doaj +3 more sources
Massively parallel functional annotation of 3' untranslated regions. [PDF]
Nat Biotechnol, 2014 Functional characterization of noncoding sequences is crucial for understanding the human genome and learning how genetic variation contributes to disease. 3' untranslated regions (UTRs) are an important class of noncoding sequences, but their functions remain largely uncharacterized.
Zhao W +5 more
europepmc +9 more sources
Argonaute binding within 3'-untranslated regions poorly predicts gene repression. [PDF]
Nucleic Acids Res, 2020 Abstract Despite two decades of study, the full scope of RNAi in mammalian cells has remained obscure. Here we combine: (i) Knockout of argonaute (AGO) variants; (ii) RNA sequencing analysis of gene expression changes and (iii) Enhanced Crosslinking Immunoprecipitation Sequencing (eCLIP-seq) using anti-AGO2 antibody to identify ...
Chu Y +5 more
europepmc +5 more sources
Expression of distinct RNAs from 3' untranslated regions. [PDF]
Nucleic Acids Res, 2011 The 3' untranslated regions (3'UTRs) of eukaryotic genes regulate mRNA stability, localization and translation. Here, we present evidence that large numbers of 3'UTRs in human, mouse and fly are also expressed separately from the associated protein-coding sequences to which they are normally linked, likely by post-transcriptional cleavage.
Mercer TR +12 more
europepmc +6 more sources
Improved annotation of 3' untranslated regions and complex loci by combination of strand-specific direct RNA sequencing, RNA-Seq and ESTs. [PDF]
PLoS ONE, 2014 The reference annotations made for a genome sequence provide the framework for all subsequent analyses of the genome. Correct and complete annotation in addition to the underlying genomic sequence is particularly important when interpreting the results ...
Nicholas J Schurch +9 more
doaj +8 more sources
Genetic Polymorphisms in the 3′-Untranslated Regions of SMAD5, FN3KRP, and RUNX-1 Are Associated with Recurrent Pregnancy Loss [PDF]
Biomedicines, 2022 Recurrent pregnancy loss (RPL) is typically defined as two or more consecutive pregnancy losses prior to 20 weeks of gestation. Although the causes of idiopathic RPL are not completely understood, vascular development and glucose concentration were ...
Min-Jung Kwon +7 more
doaj +2 more sources