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Complete Membrane Fractionation of 3T3-L1 Adipocytes
Cold Spring Harbor Protocols, 2016Fractionation techniques can facilitate the isolation of intracellular organelles containing insulin-sensitive glucose transporter isoform 4 (GLUT4), which is mobilized from GLUT4 storage vesicles in fat and muscle cells in response to insulin. This protocol for the full membrane fractionation of 3T3-L1 adipocytes results in five distinct fractions.
Jessica B A, Sadler +3 more
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Protocol for Effective Differentiation of 3T3-L1 Cells to Adipocytes
Analytical Biochemistry, 2012In this note, we present a detailed procedure for highly effective and reproducible 3T3-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, 3T3-L1 cells are widely used for studying adipogenesis and the biochemistry of adipocytes. However, using different kits and protocols published so far, we were not able
Martin Wabitsch +2 more
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The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes
2009Type 2 diabetes (T2D) is one of the fastest growing threats to human health in westernised and developing countries and is associated with central obesity, atherosclerosis, dyslipidaemia, hyperinsulinaemia and hypertension. Insulin resistance, defined as a diminished response to ordinary levels of circulating insulin in one or more peripheral tissues ...
Nicky, Konstantopoulos +1 more
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Regulation of adipocyte differentiation of 3T3-L1 cells by PDZRN3
American Journal of Physiology-Cell Physiology, 2013PDZRN3, a member of the PDZRN (or LNX) family of proteins, is essential for the differentiation of mesenchymal stem cells into myotubes, but it plays an inhibitory role in the differentiation of these cells into osteoblasts. Given that mesenchymal stem cells also differentiate into adipocytes, we examined the possible role of PDZRN3 in adipogenesis in
Takeshi, Honda +2 more
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MicroRNA-23a regulates 3T3-L1 adipocyte differentiation
Gene, 2016MicroRNAs (miRNAs) are small, non-coding RNAs, which are involved in regulation of a variety of biological processes. Since previous studies regarding the role of miRNAs in the regulation of adipogenic differentiation have shown that miRNA-27a, one member of miRNA-23a∼27a∼24 cluster, could suppress adipogenesis.
Linyuan, Shen +9 more
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The Effects of AICAR on Adipocyte Differentiation of 3T3-L1 Cells
Biochemical and Biophysical Research Communications, 2001The AMP-activated protein kinase (AMPK) activator, 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR), has been found to inhibit the differentiation of 3T3-L1 adipocytes, if added at an early phase of differentiation. AICAR blocks the expression of the late adipogenic markers, fatty acid synthase and acetyl-CoA carboxylase, and of the transcription ...
S A, Habinowski, L A, Witters
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Glucosamine-induced insulin resistance in 3T3-L1 adipocytes
American Journal of Physiology-Endocrinology and Metabolism, 2000To study molecular mechanisms for glucosamine-induced insulin resistance, we induced complete and reversible insulin resistance in 3T3-L1 adipocytes with glucosamine in a dose- and time-dependent manner (maximal effects at 50 mM glucosamine after 6 h). In these cells, glucosamine impaired insulin-stimulated GLUT-4 translocation.
E, Heart, W S, Choi, C K, Sung
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Glyburide action in cultured 3T3-L1 adipocytes.
Biochemistry international, 1986During adipocyte differentiation of 3T3-L1 cells, glyburide increased the specific activity (mU/mg protein) of glycerol-3-P dehydrogenase (by at least 14-fold) and glutamine synthetase (by 5-fold). The glyburide-mediated increases in enzyme activities were greater in the presence than in the absence of insulin.
MILLER, RE, BHANDARI, B
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Activin B inhibits lipolysis in 3T3-L1 adipocytes
Biochemical and Biophysical Research Communications, 2010Activin B, consisting of two inhibin betaB (INHBB) subunits, is a hormone known to affect gonadal function, reproduction and fetal development. We have reported that INHBB and activin B receptors are highly expressed in adipocytes suggesting that activin B may have local effects in adipose tissue.
Björn, Magnusson +3 more
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Nebivolol stimulates mitochondrial biogenesis in 3T3-L1 adipocytes
Biochemical and Biophysical Research Communications, 2013Nebivolol is a third-generation β-adrenergic receptor (β-AR) blocker with additional beneficial effects, including the improvement of lipid and glucose metabolism in obese individuals. However, the underlying mechanism of nebivolol's role in regulating the lipid profile remains largely unknown.
Chenglin, Huang +4 more
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