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Protocol for effective differentiation of 3T3-L1 cells to adipocytes
Analytical Biochemistry, 2012In this note, we present a detailed procedure for highly effective and reproducible 3T3-L1 cell differentiation. Due to their potential to differentiate from fibroblasts to adipocytes, 3T3-L1 cells are widely used for studying adipogenesis and the biochemistry of adipocytes. However, using different kits and protocols published so far, we were not able
Katja, Zebisch +3 more
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Phosphorylated glucosamine inhibits adipogenesis in 3T3-L1 adipocytes
The Journal of Nutritional Biochemistry, 2010Phosphorylated glucosamine (glucosamine-6-phosphate, PGlc) was synthesized using methanesulfonic acid, phosphorus pentoxide (P(2)O(5)), NH(2)NH(2) and DMF. Its inhibitory effect on lipid accumulation in cultured 3T3-L1 adipocytes was investigated by measuring triglyceride contents and Oil Red O staining.
Chang-Suk, Kong +3 more
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Salacia reticulata inhibits differentiation of 3T3-L1 adipocytes
Journal of Ethnopharmacology, 2011ETHOPARMACOLOGICAL RELEVANCE: Salacia reticulata, a herbal medicine which has been used for the treatment of early diabetes in Ayurvedic medicine, is reported to have an anti-obesity effect and to be useful in the treatment of diabetes mellitus, insulin resistance and other metabolic diseases.The present study was performed to elucidate the mechanism ...
Tsutomu, Shimada +8 more
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3T3-L1 Adipocytes promote the growth of mammary epithelium
Experimental Cell Research, 1984Murine mammary epithelium grows in association with predominantly adipocyte stroma in vivo. To investigate potential growth-promoting effects of adipocytes on mammary epithelium, we developed a co-culture system of mammary epithelium and adipocytes by taking advantage of the 3T3-L1 cell line.
Levine, J F, Stockdale, F E
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Nobiletin enhances differentiation and lipolysis of 3T3-L1 adipocytes
Biochemical and Biophysical Research Communications, 2007Nobiletin is a polymethoxylated flavone found in certain citrus fruits. Here we demonstrate that nobiletin enhance differentiation of 3T3-L1 preadipocytes. Nobiletin dose-dependently increased accumulation of lipid droplets in adipocytes. Quantitative RT-PCR analyses indicated that nobiletin increased the expression of genes critical for acquisition of
Takeshi, Saito +2 more
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ATF3 inhibits adipocyte differentiation of 3T3-L1 cells
Biochemical and Biophysical Research Communications, 2012ATF3 is a stress-adaptive gene that regulates proliferation or apoptosis under stress conditions. However, the role of ATF3 is unknown in adipocyte cells. Therefore, in this study, we investigated the functional role of ATF3 in adipocytes. Both lentivirus-mediated overexpression of ATF3 and stably-overexpressed ATF3 inhibited adipocyte differentiation ...
Min Kyung, Jang +3 more
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Inhibitory Effects of Fucoidan in 3T3-L1 Adipocyte Differentiation
Marine Biotechnology, 2008Fucoidan is a group of sulfated fucose-containing polysaccharides that derived from non-mammalian origin such as marine brown algae, the jelly coat from sea urchin eggs, and the sea cucumber body wall. However, potential biological activities against obesity from fucoidan were not reported in the literature.
Mi-Ja, Kim, Un-Jae, Chang, Jin-Sil, Lee
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Shikonin inhibits fat accumulation in 3T3‐L1 adipocytes
Phytotherapy Research, 2009AbstractShikonin, 5,6‐dihydroxyflavone‐7‐glucuronic acid, is the main ingredient of Lithospermum erythrorhizon Sieb. et Zucc, and was reported to have various biological activities including antiinflammatory, anticancer, antimicrobial and others. This study aimed to elucidate, for the first time, the antiobesity activity of shikonin and its mechanism ...
Haeyong, Lee +2 more
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Differentiation of 3T3-L1 fibroblasts to adipocytes
Experimental Cell Research, 1979Tong-Hsuan Chang +2 more
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