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The effect of temperature on metabolism in 3T3 cells and SV40-transformed 3T3 cells as measured by microcalorimetry

Thermochimica Acta, 1990
Abstract The rate of heat production was measured at different temperatures for nontransformed and SV40-transformed 3T3 cells. Both cell lines were very similar with respect to metabolic temperature dependence in the lower temperature range. The apparent activation energy for 3T3 cells and SV40-transformed 3T3 cells was 60 kJ/mol and 66 kJ/mol ...
Per Lönnbro, Arne Schön
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DNA breakdown of 3T3 and SV 40-transformed 3T3 cells cultured in suspension

Cell Biology International Reports, 1987
It was examined what effect of suspension culture exerted on prelabeled DNA of 3T3 and SV 40 transformed cells (SV3T3). On an alkaline sucrose density gradient the small size DNA of 3T3 cells increased with time of suspension, while that of SV3T3 did not.
T, Matsuhisa, Y, Mori
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Plasmin-mediated agglutination by concanavalin A of 3T3 cells cocultured with SV40–3T3 transformants

Nature, 1976
REICH and colleagues have reported that a variety of embryo fibroblasts in tissue culture show fibrinolytic activity after viral transformation1,2. This effect is attributable to the action of a plasminogen activator, which is a serine protease3 and is present at higher concentrations in transformed than untransformed fibroblasts4; this activator ...
P, Whur   +3 more
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Protease Inhibitors in 3T3 Cells

1984
A 44K anti-thrombin-like protease inhibitor, termed protease-nexin, has recently been discovered in the culture medium of human fibroblasts (Baker et al., 1980). It forms heparin-dependent covalent complexes with the catalytic site of serine proteases.
Gabriella Fibbi   +2 more
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Modulation of the cell cycle during reversible growth inhibition of 3T3 and SV40-3T3 cells with succinylated concanavalin A

Experimental Cell Research, 1980
Abstract Succinylated concanavalin A (ConA), a non-toxic, non-agglutinating derivative of the jack bean lectin ConA inhibits the growth of both normal and SV40-transformed 3T3 cells. The quantitative but not the qualitative growth inhibitory effect of succinyl-ConA can be modulated by the composition of the growth medium.
K, Ballmer, R J, Mannino, M M, Burger
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Transformation-associated changes in nuclear-coded mitochondrial proteins in 3T3 cells and SV40-transformed 3T3 cells

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1984
Comparative two-dimensional gel electrophoretic studies were performed on mitochondrial proteins in nontransformed mouse 3T3 cells and in SV40-transformed 3T3 cells, SV-T2. Two polypeptides, of 58 and 40 kDa, were present in increased amounts in SV40-transformed cells.
S H, Zuckerman   +2 more
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Cell growth and tRNA4lys synthesis in mouse 3T3 cells

Experimental Cell Research, 1982
Abstract The RPC-5 chromatographic profiles of lys-tRNA were analyzed during the growth of 3T3 cells in culture. An inverse relationship was seen between tRNA2lys and tRNA4lys which was markedly influenced by medium changes. This interchange of tRNA2lys and tRNA4lys could be controlled by altering the levels of serum in the medium, or more precisely ...
Ortwerth, B J   +3 more
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THE EFFECTS OF INTERFERON ON 3T3‐L1 CELL DIFFERENTIATION*

Annals of the New York Academy of Sciences, 1980
Mouse 3T3-L1 fibroblasts, after attaining confluence, slowly differentiate functionally and morphologically into adipose cells. This conversion, characterized by a great accumulation of lipids, mostly triglycerides, is hastened by added insulin. The cells are relatively sensitive to the antiviral action of mouse fibroblast interferon (IF).
S E, Grossberg, S, Keay
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Prostaglandin production by 3T3-L1 cells in culture

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
Rapidly growing cultures of 3T3-L1 preadipocytes produce large quantities of prostaglandins when they are either stimulated with the calcium ionophore A23187 or incubated with arachidonic acid. The main prostaglandin produced under all conditions was prostaglandin E2.
B T, Hyman, L L, Stoll, A A, Spector
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Growth hormone and the adipose conversion of 3T3 cells

Cell, 1982
Cultured preadipose 3T3 cells are induced or enabled to undergo adipose conversion in the presence of an extract of pituitary gland. Adipogenic activity is found in standard growth hormone preparations derived from different species. Further purification of rat growth hormone by several methods does not remove its adipogenic activity.
M, Morikawa, T, Nixon, H, Green
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