Results 1 to 10 of about 4,980,683 (248)

Matrigel® enhances 3T3-L1 cell differentiation [PDF]

Adipocyte, 2021
Culturing cells on bio-gels are believed to provide a more in vivo-like extracellular matrix. 3T3-L1 cells cultured on Matrigel® significantly alteregd their proliferation and differentiation as compared to growth on tissue culture-coated polystyrene ...
Chitmandeep Josan, Sachin Kakar, Sandeep Raha   +2 more
doaj   +3 more sources

TGF‐β effects on adipogenesis of 3T3‐L1 cells differ in 2D and 3D cell culture conditions [PDF]

FEBS Open Bio
The TGF‐β superfamily plays a pivotal role in the regulation of adipogenesis, but little is known about the potential differential role of the three isoforms of TGF‐β, TGF‐β‐1~3.
Araya Umetsu, Megumi Watanabe, Tatsuya Sato, Megumi Higashide, Nami Nishikiori, Masato Furuhashi, Hiroshi Ohguro   +6 more
doaj   +2 more sources

Type III Collagen is Required for Adipogenesis and Actin Stress Fibre Formation in 3T3-L1 Preadipocytes

Biomolecules, 2021
GPR56 is required for the adipogenesis of preadipocytes, and the role of one of its ligands, type III collagen (ColIII), was investigated here. ColIII expression was examined by reverse transcription quantitative polymerase chain reaction, immunoblotting
Mohammad Al Hasan, Patricia E. Martin, Xinhua Shu, Steven Patterson, Chris Bartholomew   +4 more
doaj   +1 more source

MiR-146a-5p, targeting ErbB4, promotes 3T3-L1 preadipocyte differentiation through the ERK1/2/PPAR-γ signaling pathway

Lipids in Health and Disease, 2022
Background MicroRNAs (MiRNAs) are known to participate in preadipocyte differentiation, but the manner in which miR-146a-5p participates in this process remains unclear.
Yifen Wang, Jie Zhang, Xueru Chu, Mengke Wang, Yongning Xin, Shousheng Liu   +5 more
doaj   +1 more source

Characterizing 3T3-L1 MBX Adipocyte Cell Differentiation Maintained with Fatty Acids as an In Vitro Model to Study the Effects of Obesity

Life, 2023
The increasing prevalence of obesity has prompted intensive research into understanding its role in pathogenesis and designing appropriate treatments. To determine the signals generated from the interaction of fat cells with a target organ, a reliable ...
Noshin Mubtasim, Lauren Gollahon
doaj   +1 more source

Prolonged induction activates Cebpα independent adipogenesis in NIH/3T3 cells. [PDF]

PLoS ONE, 2013
BACKGROUND: 3T3-L1 cells are widely used to study adipogenesis and insulin response. Their adipogenic potential decreases with time in the culture. Expressing exogenous genes in 3T3-L1 cells can be challenging.
Hsiao-Yun Shao, Hsue-Yin Hsu, Kuan-Sju Wu, Siow-Wey Hee, Lee-Ming Chuang, Jih-I Yeh   +5 more
doaj   +1 more source

Mouse mesoderm-specific transcript inhibits adipogenic differentiation and induces trans-differentiation into hepatocyte-like cells in 3T3-L1 preadiocytes

BMC Research Notes, 2022
Objective The mesoderm-specific transcript (Mest) is an imprinted gene that is transcribed from the paternal allele. It is a marker of adipose tissue expansion; however, it is uncertain whether Mest expression promotes or suppresses adipogenic ...
Yoshito Kadota, Takashige Kawakami, Masao Sato, Shinya Suzuki   +3 more
doaj   +1 more source

Investigating the role of EPA and DHA on cellular oxidative stress; profiling antidiabetic and antihypertensive potential

Journal of Pharmacy and Bioallied Sciences, 2022
Background: Polyunsaturated fatty acids (PUFAs) remain part of the diet and are essential for growth and development. Furthermore, omega − 3 fatty acids boost various cardiovascular disease risk factors as well as lower blood pressure and cholesterol ...
Fahad K Aldhafiri
doaj   +1 more source

Construction of stable coxsackievirus and adenovirus receptor-expressing 3T3-L1 cells

Journal of Lipid Research, 2001
3T3-L1 cells have been used as a model to study the differentiation and physiology of adipocytes. Exogenous expression of proteins in these cells offers the prospect of understanding the protein's function(s) in adipose tissue.
David J. Orlicky, James DeGregori, Jerome Schaack   +2 more
doaj   +1 more source

A novel 3T3-L1 preadipocyte variant that expresses PPARγ2 and RXRα but does not undergo differentiation

Journal of Lipid Research, 1998
This report describes a novel adipocyte-like cell line termed 3T3-L1/RB1 that was derived from preadipocyte cell line, 3T3-L1. The 3T3-L1/RB1 cells continued to divide after reaching confluence, formed foci, and constitutively expressed a low level of ...
Rebecca A. Baillie, Xiaoming Sha, Philippe Thuillier, Steven D. Clarke   +3 more
doaj   +1 more source