Results 251 to 260 of about 4,897,037 (304)

Proliferative activity and ribosomal RNA content of 3T3 and SV40-3T3 cells

Cell Biology International Reports, 1983
The phenomenon of resistance of confluent 3T3 cells to serum stimulation of proliferation is investigated and shown to depend drastically on the kinetics of application of growth factors with the serum. The cellular content of ribosomal RNA is determined flow-cytometrically by measuring the red fluorescence of cells stained with acridine orange ...
G, Adam, U, Steiner, K, Seuwen
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Synchronization of mouse 3T3 and SV40 3T3 cells by way of centrifugal elutriation

Experimental Cell Research, 1977
Populations of G1 phase 3T3 and SV40 3T3 mouse fibroblasts have been isolated from exponentially growing cultures by the technique of centrifugal elutriation. Return of the G1 phase cells to growth conditions results in their synchronous passage through the cell cycle, as determined from monitoring of cell number, [3H]thymidine ([3H]TdR) incorporation ...
Mitchell, B F, Tupper, J T
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Pyridine nucleotide synthesis in 3T3 cells

Journal of Cellular Physiology, 1979
AbstractThe biosynthesis of NAD has been examined in 3T3 cells. The net synthesis of pyridine nucleotides does not occur when cells are cultured in the absence of performed pyridine ring compounds; however, growth continues normally for up to four cell doublings resulting in cells with a total pyridine nucleotide content that is reduced by as much as ...
E L, Jacobson, R A, Lange, M K, Jacobson
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Cholesterol levels and plasma membrane fluidity in 3T3 and SV101‐3T3 cells

Journal of Supramolecular Structure, 1979
AbstractPolyene antibiotics such as filipin selectively inhibit wheat germ agglutinin‐induced agglutination of transformed and malignant cells compared to normal cells (Hatten ME, Burger MM: Biochemistry 18:739, 1979). Since filipin binds specifically to cholesterol, we measured cholesterol levels in 3T3 cells and SV101‐3T3 cells.
C J, Scandella, J A, Hayward, N, Lee
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Control of 3T3 cell proliferation by calcium

In Vitro, 1974
When a population of 3T3 mouse cells was subcultured regularly at confluency, the original epitheliodid or stellate cells disappeared and, by the ninth passage, they had been replaced by spindle-shaped cells. The original cells proliferated only when the extracellular calcium concentration exceeded 0.1mm, and their proliferative activity became maximum
Boynton, A., Whitfield, James, Isaacs, R., Morton, H.   +3 more
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Selective cytotoxicity for transformed 3T3 cells

Nature, 1976
THE therapeutic usefulness of many anti-cancer agents depends on their selective toxicity for cells in the S (DNA synthesising) phase of the cell cycle1. Thus, rapidly proliferating cancer cells are more effectively killed by these agents than are normal cell populations, which have a lower proportion of cells in S.
E, Rozengurt, C C, Po
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The phagokinetic tracks of 3T3 cells

Cell, 1977
This paper describes a technique of visualizing tracks of cultured cells moving on a glass substrate covered with gold particles. It leads to the following observations: -If the tracks of many cells are examined, cell line characteristic track patterns become apparent. -In several cases, second or third generation descendents of 3T3 cells were observed
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Regulation of passive membrane permeability for potassium ions by cell density of 3T3 and SV40-3T3 cells

Experimental Cell Research, 1979
Abstract The passive K + permeability of 3T3 and SV40-3T3 cells was evaluated from experiments on passive K + efflux and electrical transmembrane potential measurements at different cell growth densities, external calcium concentrations and temperatures.
G, Adam, M, Ernst, J P, Seher
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Cell cycle parameters of 3T3 cells cultured as aggregates

In Vitro, 1981
BALB/c3T3 cells cultured as aggregates were examined by two independent techniques to determine whether or not cells accumulated at a specific point in the cell cycle, and if so to determine the point at which they accumulate. Replating cells onto dishes followed by pulse labeling with [3H]thymidine and autoradiography indicated that aggregate-cultured
H, Gershman, H A, Crissman, D A, Carrino
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Control by cell interaction of phosphate uptake in 3T3 cells

Experimental Cell Research, 1975
Abstract Phosphate uptake by monolayers of 3T3 cell decreases when the cultures enter the stationary phase, even when incubated in fresh medium containing 10% serum. However, SV 3T3 cultures retain a high rate of phosphate uptake when the cells reach saturation densities.
L, Harel, M, Jullien, C, Blat
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