Results 31 to 40 of about 12,448,100 (233)

Chemo‐Enzymatic Fluorescence Labeling Of Genomic DNA For Simultaneous Detection Of Global 5‐Methylcytosine And 5‐Hydroxymethylcytosine **

ChemBioChem, 2023
5‐Methylcytosine and 5‐hydroxymethylcytosine are epigenetic modifications involved in gene regulation and cancer. We present a new, simple, and high‐throughput platform for multi‐color epigenetic analysis.
S. Avraham, Leonie Schütz, Larissa Käver, Andreas Dankers, Sapir Margalit, Yael Michaeli, S. Zirkin, Dmitry Torchinsky, Noa Gilat, Omer Bahr, Gil Nifker, M. Koren-Michowitz, E. Weinhold, Y. Ebenstein   +13 more
semanticscholar   +1 more source

Cell-free DNA 5-hydroxymethylcytosine is an emerging marker of acute myeloid leukemia

Scientific Reports, 2022
Aberrant changes in 5-hydroxymethylcytosine (5hmC) are a unique epigenetic feature in many cancers including acute myeloid leukemia (AML). However, genome-wide analysis of 5hmC in plasma cell-free DNA (cfDNA) remains unexploited in AML patients.
Jianming Shao, Sihan Wang, Diana C. West-Szymanski, J. Karpus, Shilpan S. Shah, S. Ganguly, Janice L. Smith, Y. Zu, Chuan He, Zejuan Li   +9 more
semanticscholar   +1 more source

Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI [PDF]

, 2017
Background: Global epigenetic reprogramming is considered to be essential during embryo development to establish totipotency. In the classic model first described in the mouse, the genome-wide DNA demethylation is asymmetric between the paternal and the ...
De Schauwer, Catharina, Heras Garcia, Sonia, Smits, Katrien, Van Soom, Ann   +3 more
core   +1 more source

Spurious transcription causing innate immune responses is prevented by 5-hydroxymethylcytosine

Nature Genetics, 2022
Generation of functional transcripts requires transcriptional initiation at regular start sites, avoiding production of aberrant and potentially hazardous aberrant RNAs.
Fan Wu, Xiang Li, M. Looso, Hang Liu, D. Ding, S. Günther, C. Kuenne, Shuya Liu, N. Weissmann, T. Boettger, A. Atzberger, S. Kolahian, H. Renz, S. Offermanns, U. Gärtner, M. Potente, Yonggang Zhou, Xuejun Yuan, T. Braun   +18 more
semanticscholar   +1 more source

5-hydroxymethylcytosine profiling as an indicator of cellular state [PDF]

, 2013
A Laird is supported by the Medical Research Council Scottish Clinical Pharmacology and Pathology Programme, The Royal College of Surgeons of Edinburgh Robertson’s Trust and The Melville Trust for the Care and Cure of Cancer.
Harrison, David J., Laird, Alexander, Meehan, Richard R., Thomson, John P.   +3 more
core   +1 more source

Tet-assisted bisulfite sequencing of 5-hydroxymethylcytosine [PDF]

Nature Protocols, 2012
A complete understanding of the potential function of 5-hydroxymethylcytosine (5-hmC), a DNA cytosine modification in mammalian cells, requires an accurate single-base resolution sequencing method. Here we describe a modified bisulfite-sequencing method, Tet-assisted bisulfite sequencing (TAB-seq), which can identify 5-hmC at single-base resolution, as
Miao, Yu, Gary C, Hon, Keith E, Szulwach, Chun-Xiao, Song, Peng, Jin, Bing, Ren, Chuan, He   +6 more
openaire   +2 more sources

TET2 inhibits differentiation of embryonic stem cells but does not overcome methylation-induced gene silencing [PDF]

, 2014
TET2 is a methylcytosine dioxygenase that is frequently mutated in myeloid malignancies, notably myelodysplasia and acute myeloid leukemia. TET2 catalyses the conversion of 5′-methylcytosine to 5′-hydroxymethylcytosine within DNA and has been implicated ...
Chevassut, Timothy, Norman, Louis, Tarrant, Paul   +2 more
core   +3 more sources

5-hydroxymethylcytosine is dynamically regulated during forebrain organoid development and aberrantly altered in Alzheimer’s disease

Cell Reports, 2021
SUMMARY 5-hydroxymethylcytosine (5hmC) undergoes dynamic changes during mammalian brain development, and its dysregulation is associated with Alzheimer’s disease (AD).
Janise N. Kuehner, Junyu Chen, Emily C. Bruggeman, Feng Wang, Yangping Li, Chongchong Xu, Zachary T. McEachin, Ziyi Li, Li Chen, Chadwick M. Hales, Z. Wen, Jingjing Yang, Bing Yao   +12 more
semanticscholar   +1 more source

Genome-wide characterization of cytosine-specific 5-hydroxymethylation in normal breast tissue

Epigenetics, 2020
Despite recent evidence that 5-hydroxymethylcytosine (5hmC) possesses roles in gene regulation distinct from 5-methylcytosine (5mC), relatively little is known regarding the functions of 5hmC in mammalian tissues.
Owen M. Wilkins, Kevin C. Johnson, E. Andres Houseman, Jessica E. King, Carmen J. Marsit, Brock C. Christensen   +5 more
doaj   +1 more source

Cell‐free DNA 5‐hydroxymethylcytosine as a marker for common cancer detection

Clinical and Translational Discovery, 2022
Background Early cancer detection can dramatically improve clinical outcomes and greatly reduce economic burden of patients. Plasma cell–free DNA (cfDNA) 5‐hydroxymethylcytosine (5hmC) is an emerging epigenetic marker for cancer diagnosis.
Jianming Shao, Eric H. Bernicker, Chuan He, Zejuan Li   +3 more
doaj   +1 more source