Results 41 to 50 of about 12,448,100 (233)

Integrated single-cell sequencing of 5-hydroxymethylcytosine and genomic DNA using scH&G-seq

STAR Protocols, 2021
Summary: The asymmetric distribution of 5-hydroxymethylcytosine (5hmC) between two DNA strands of a chromosome enables endogenous reconstruction of cellular lineages at an individual-cell-division resolution. Further, when integrated with data on genomic
Alex Chialastri, Chatarin Wangsanuwat, Siddharth S. Dey   +2 more
doaj   +1 more source

Loss of 5-hydroxymethylcytosine correlates with increasing morphologic dysplasia in melanocytic tumors [PDF]

, 2015
DNA methylation is the most well studied epigenetic modification in cancer biology. 5-hydroxymethylcytosine is an epigenetic mark that can be converted from 5-methylcytosine by the ten-eleven translocation gene family.
Dresser, Karen, Larson, Allison R., Lezcano, Cecilia, Lian, Christine Guo, Mihm, Martin C., Murphy, George F., Scolyer, Richard A., Shi, Yujiang G., Thompson, John F., Woda, Bruce A., Yosufi, Benafsha, Zhan, Qian   +11 more
core   +1 more source

TET Enzymes and 5-Hydroxymethylcytosine in Neural Progenitor Cell Biology and Neurodevelopment

Frontiers in Cell and Developmental Biology, 2021
Studies of tissue-specific epigenomes have revealed 5-hydroxymethylcytosine (5hmC) to be a highly enriched and dynamic DNA modification in the metazoan nervous system, inspiring interest in the function of this epigenetic mark in neurodevelopment and ...
Ian C. MacArthur, Meelad M. Dawlaty
semanticscholar   +1 more source

Alterations of 5-Hydroxymethylcytosine in Human Cancers [PDF]

Cancers, 2013
Prior to 2009, 5-methylcytosine (5-mC) was thought to be the only biologically significant cytosine modification in mammalian DNA. With the discovery of the TET enzymes, which convert 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC), however, intense interest has emerged in determining the biological function of 5-hmC.
Mariani, Christopher J., Madzo, Jozef, Moen, Erika L., Yesilkanal, Ali, Godley, Lucy A.   +4 more
openaire   +2 more sources

Dynamics of 5-hydroxymethylcytosine during mouse spermatogenesis [PDF]

Nature Communications, 2013
Little is known about how patterns of DNA methylation change during mammalian spermatogenesis. 5 hmC has been recognized as a stable intermediate of DNA demethylation with potential regulatory functions in the mammalian genome. However, its global pattern in germ cells has yet to be addressed.
Haiyun, Gan, Lu, Wen, Shangying, Liao, Xiwen, Lin, Tingting, Ma, Jun, Liu, Chun-Xiao, Song, Min, Wang, Chuan, He, Chunsheng, Han, Fuchou, Tang   +10 more
openaire   +2 more sources

5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.

PLoS ONE, 2012
BackgroundIn the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the ...
Juliette Salvaing, Tiphaine Aguirre-Lavin, Claire Boulesteix, Gaëtan Lehmann, Pascale Debey, Nathalie Beaujean   +5 more
doaj   +1 more source

5‐Hydroxymethylcytosine: a new kid on the epigenetic block?

Molecular Systems Biology, 2011
The discovery of the Ten‐Eleven‐Translocation (TET) oxygenases that catalyze the hydroxylation of 5‐methylcytosine (5mC) to 5‐hydroxymethylcytosine (5hmC) has triggered an avalanche of studies aiming to resolve the role of 5hmC in gene regulation if any.
Filomena Matarese, Enrique Carrillo‐de Santa Pau, Hendrik G Stunnenberg   +2 more
doaj   +1 more source

Eicosapentaenoic acid induces DNA demethylation in carcinoma cells through a TET1-dependent mechanism [PDF]

, 2018
In cancer cells, global genomic hypomethylation is found together with localized hypermethylation of CpG islands within the promoters and regulatory regions of silenced tumor suppressor genes.
Billi, Monia, Cannarile, Lorenza, Ceccarelli, Veronica, Grignani, Francesco, Ottini, Laura, Riccardi, Carlo, Ronchetti, Simona, Talesa, Vincenzo Nicola, Valentini, Virginia, Vecchini, Alba   +9 more
core   +1 more source

Detection of early stage pancreatic cancer using 5-hydroxymethylcytosine signatures in circulating cell free DNA

Nature Communications, 2020
Pancreatic cancer is often detected late, when curative therapies are no longer possible. Here, we present non-invasive detection of pancreatic ductal adenocarcinoma (PDAC) by 5-hydroxymethylcytosine (5hmC) changes in circulating cell free DNA from a ...
G. Guler, Y. Ning, C. Ku, T. Phillips, E. McCarthy, C. Ellison, A. Bergamaschi, F. Collin, P. Lloyd, A. Scott, M. Antoine, Wendy Wang, K. Chau, A. Ashworth, S. Quake, S. Levy   +15 more
semanticscholar   +1 more source

5-Hydroxymethylcytosine Signatures in Circulating Cell-Free DNA as Diagnostic Biomarkers for Late-Onset Alzheimer’s Disease

Journal of Alzheimer's Disease, 2021
Background: 5-Hydroxymethylcytosine (5hmC) is an epigenetic DNA modification that is highly abundant in central nervous system. It has been reported that DNA 5hmC dysregulation play a critical role in Alzheimer’s disease (AD) pathology.
Lei Chen, Shunliang Xu, Q. Shen, Hongzhuan Yu, Shengjie Pei, Yangting Zhang, Xin He, Qiuzhen Wang, Duo Li   +8 more
semanticscholar   +1 more source