Results 11 to 20 of about 29,834 (207)
Determination of the presence of 5-methylcytosine in Paramecium tetraurelia. [PDF]
PLoS ONE, 2018 5-methylcytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization ...
Aditi Singh +5 more
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Immunodetection of 5′-methylcytosine on Giemsa-stained chromosomes [PDF]
BioTechniques, 2005 BioTechniques 527 In higher vertebrates, the major, if not the only, covalent modification of DNA is a methyl-group present at the C5 position of cytosine yielding 5′-methylcytosine (5′-MC). This modification occurs only in the context of CpG dinucleotides, short sequences that are significantly underrepresented in the genome but are found frequently ...
Werner Pfarr +3 more
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5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
Dermatopathology, 2021 Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in ...
Alexandre Stahl +5 more
doaj +1 more source
Levels of DNA cytosine methylation in the Drosophila genome [PDF]
PeerJ, 2018 Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation.
Saniya Deshmukh +4 more
doaj +2 more sources
Discovery of a Bacterial 5-Methylcytosine Deaminase [PDF]
Biochemistry, 2014 5-Methylcytosine is found in all domains of life, but the bacterial cytosine deaminase from Escherichia coli (CodA) will not accept 5-methylcytosine as a substrate. Since significant amounts of 5-methylcytosine are produced in both prokaryotes and eukaryotes, this compound must eventually be catabolized and the fragments recycled by enzymes that have ...
Hitchcock, Daniel S. +4 more
openaire +2 more sources
Characterizing 5-methylcytosine in the mammalian epitranscriptome [PDF]
Genome Biology, 2013 The post-transcriptional modification 5-methylcytosine (m5C) occurs in a wide range of coding and non-coding RNAs. We describe transcriptome-wide approaches to capture the global m5C RNA methylome. We also discuss the potential functions of m5C in RNA and compare them to 6-methyladenosine modifications.
Hussain S +4 more
openaire +2 more sources
International Journal of Genomics, 2020 DNA methylation is an epigenetic mechanism used by cells to control gene expression. DNA methylation is a commonly used epigenetic signaling tool that can hold genes in the “off” position.
Ahmed M. Awad +3 more
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A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
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Detection of 5-methylcytosine in DNA sequences [PDF]
Nucleic Acids Research, 1978 Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so ...
H, Ohmori, J I, Tomizawa, A M, Maxam
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Frontiers in Oncology, 2023 ObjectiveTo explore the functions of the polymorphisms in 5-methylcytosine (m5C) modification-related coding genes on the susceptibility of pediatric acute lymphoblastic leukemia (ALL).MethodsCase–control study and multinomial logistic regression ...
Xueliang Wang +9 more
doaj +1 more source