Results 11 to 20 of about 44,474 (235)
Immunodetection of 5′-methylcytosine on Giemsa-stained chromosomes [PDF]
BioTechniques, 2005 BioTechniques 527 In higher vertebrates, the major, if not the only, covalent modification of DNA is a methyl-group present at the C5 position of cytosine yielding 5′-methylcytosine (5′-MC). This modification occurs only in the context of CpG dinucleotides, short sequences that are significantly underrepresented in the genome but are found frequently ...
Werner Pfarr +3 more
doaj +3 more sources
Cytosine-to-Uracil Deamination by SssI DNA Methyltransferase [PDF]
, 2013 The prokaryotic DNA(cytosine-5)methyltransferase M.SssI shares the specificity of eukaryotic DNA methyltransferases (CG) and is an important model and experimental tool in the study of eukaryotic DNA methylation.
A Beletskii +47 more
core +11 more sources
BioChem, 2022 Three forms of methylated cytosines are present in the eukaryotic genome: 3-methylcytosine, 4-methylcytosine and 5-methylcytosine. 3-methylcytosines create methyl lesions, which impair local DNA function and flexibility, resulting in replication and ...
Aninda Sundar Dey
doaj +1 more source
5mC-hydroxylase activity is influenced by the PARylation of TET1 enzyme [PDF]
, 2015 5-hydroxymethylcytosine is a new epigenetic modification deriving from the oxidation of 5-methylcytosine by the TET hydroxylase enzymes. DNA hydroxymethylation drives DNA demethylation events and is involved in the control of gene expression ...
CAIAFA, Paola +3 more
core +12 more sources
5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
Dermatopathology, 2021 Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in ...
Alexandre Stahl +5 more
doaj +1 more source
Levels of DNA cytosine methylation in the Drosophila genome [PDF]
PeerJ, 2018 Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation.
Saniya Deshmukh +4 more
doaj +2 more sources
Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI [PDF]
, 2017 Background: Global epigenetic reprogramming is considered to be essential during embryo development to establish totipotency. In the classic model first described in the mouse, the genome-wide DNA demethylation is asymmetric between the paternal and the ...
De Schauwer, Catharina +3 more
core +1 more source
International Journal of Genomics, 2020 DNA methylation is an epigenetic mechanism used by cells to control gene expression. DNA methylation is a commonly used epigenetic signaling tool that can hold genes in the “off” position.
Ahmed M. Awad +3 more
doaj +1 more source
A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
doaj +1 more source
Theoretical and experimental study of the vibrational spectra of 1.5-dimethylcytosine [PDF]
, 2008 The Raman spectra of the solid 1,5-dimethylcytosine and the FTIR spectra at room and low temperatures respectively have been registered. Quantum mechanical calculations of energies, geometries and vibrational wavenumbers were carried out by using ab ...
Ben Altabef, Aida +4 more
core +1 more source