Results 11 to 20 of about 11,684,120 (271)
A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine. [PDF]
PLoS ONE, 2014 We demonstrate that a mutant of uracil DNA glycosylase (N123D:L191A) distinguishes between cytosine and methylcytosine. Uracil DNA glycosylase (UDG) efficiently removes uracil from DNA in a reaction in which the base is flipped into the enzyme's active ...
Scott T Kimber, Tom Brown, Keith R Fox
doaj +3 more sources
Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
Nature Communications, 2017 Transcription activator-like effector proteins recognise specific DNA sequences via tandem repeats. Here the authors demonstrate TALEs can recognise the methylated bases 5mC and 5hmC, enabling them to detect epigenetic modifications.
Yuan Zhang +7 more
doaj +3 more sources
Selective Functionalisation of 5-Methylcytosine by Organic Photoredox Catalysis. [PDF]
, 2023 The epigenetic modification 5-methylcytosine plays a vital role in development, cell specific gene expression and disease states. The selective chemical modification of the 5-methylcytosine methyl group is challenging. Currently, no such chemistry exists.
core +11 more sources
Mbd4 and MutSĪ± protect cells from spontaneous deamination of 5-methylcytosine. [PDF]
Nucleic Acids Res5-Methylcytosine (5mC) is a common source of somatic mutations. Deamination of 5mC to thymine generates a G/T mismatch, which occurs spontaneously and must be repaired prior to DNA replication to avoid mutation.
Bilardi RA +5 more
europepmc +2 more sources
Frontiers in Genetics, 2022 Background: 5-methylcytosine has a profound impact on the development and progression of hepatocellular carcinoma. The aim of this study was to investigate the usefulness of 5-methylcytosine in determining the prognosis, tumor microenvironment, and ...
Mingyuan Luan +4 more
doaj +1 more source
Identification and validation of 5-methylcytosine-associated genes in diffuse large B-cell lymphoma
Heliyon, 2023 5-methylcytosine modifications play a significant role in carcinogenesis; however, studies exploring 5-methylcytosine-related genes in diffuse large B-cell lymphoma patients are lacking.
Cheng Xing +14 more
doaj +1 more source
BioChem, 2022 Three forms of methylated cytosines are present in the eukaryotic genome: 3-methylcytosine, 4-methylcytosine and 5-methylcytosine. 3-methylcytosines create methyl lesions, which impair local DNA function and flexibility, resulting in replication and ...
Aninda Sundar Dey
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5-Hydroxymethylcytosine Loss in Conjunctival Melanoma
Dermatopathology, 2021 Aims: Conjunctival and cutaneous melanoma partially share similar clinical and molecular backgrounds. As 5-hydroxymethylcytosine (5-hmC) loss has been demonstrated in cutaneous melanoma, we decided to assess if similar changes were occurring in ...
Alexandre Stahl +5 more
doaj +1 more source
Detection of 5-methylcytosine in DNA sequences [PDF]
Nucleic Acids Research, 1978 Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so ...
H, Ohmori, J I, Tomizawa, A M, Maxam
openaire +2 more sources
Levels of DNA cytosine methylation in the Drosophila genome [PDF]
PeerJ, 2018 Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation.
Saniya Deshmukh +4 more
doaj +2 more sources