Results 31 to 40 of about 44,474 (235)
Repeats as global DNA methylation marker in bovine preimplantation embryos [PDF]
, 2017 DNA methylation undergoes dynamic changes and is a crucial part of the epigenetic regulation during mammalian early development. To determine the DNA methylation levels in bovine embryos, we applied a bisulfite sequencing based method aimed at repetitive
Li, Wenwen, Peelman, Luc, Van Soom, Ann
core +2 more sources
Protein Interactions at Oxidized 5-Methylcytosine Bases
Journal of Molecular Biology, 2020 5-Methylcytosine (5mC), the major modified DNA base in mammalian cells, can be oxidized enzymatically to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) by the Ten-Eleven-Translocation (TET) family of proteins.
Pfeifer, Gerd P +2 more
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Oxygenase Catalyzed 5-Methylcytosine Hydroxylation
Chemistry & Biology, 2009 Recent reports identify the oxygenase catalyzed production of 5-hydroxymethylcytosine as a modification to mammalian DNA (Kriaucionis and Heintz, 2009; Tahiliani et al., 2009). This discovery has potential far-reaching implications for epigenetic regulation and will stimulate efforts to identify new nucleic acid modifications.
Loenarz, C, Schofield, C
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Discovery of a Bacterial 5-Methylcytosine Deaminase [PDF]
Biochemistry, 2014 5-Methylcytosine is found in all domains of life, but the bacterial cytosine deaminase from Escherichia coli (CodA) will not accept 5-methylcytosine as a substrate. Since significant amounts of 5-methylcytosine are produced in both prokaryotes and eukaryotes, this compound must eventually be catabolized and the fragments recycled by enzymes that have ...
Hitchcock, Daniel S. +4 more
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UHRF2 regulates local 5-methylcytosine and suppresses spontaneous seizures
Epigenetics, 2017 The 5-methylcytosine (5mC) modification regulates multiple cellular processes and is faithfully maintained following DNA replication. In addition to DNA methyltransferase (DNMT) family proteins, ubiquitin-like PHD and ring finger domain-containing ...
Yidan Liu +6 more
doaj +1 more source
Inhibition of DNA methyltransferase leads to increased genomic 5-hydroxymethylcytosine levels in hematopoietic cells. [PDF]
, 2018 5-Hydroxymethylcytosine (5hmC) is produced from 5-methylcytosine (5mC) by Ten-eleven translocation (TET) dioxygenases. The epigenetic modification 5hmC has crucial roles in both cellular development and differentiation.
Apró, Anna +9 more
core +4 more sources
Characterizing 5-methylcytosine in the mammalian epitranscriptome [PDF]
Genome Biology, 2013 The post-transcriptional modification 5-methylcytosine (m5C) occurs in a wide range of coding and non-coding RNAs. We describe transcriptome-wide approaches to capture the global m5C RNA methylome. We also discuss the potential functions of m5C in RNA and compare them to 6-methyladenosine modifications.
Hussain S +4 more
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DNA 5-Methylcytosine-Specific Amplification and Sequencing
Journal of the American Chemical Society, 2020 DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input ...
Chang Liu +9 more
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Advanced Science, 2023 Methylating RNA post‐transcriptionally is emerging as a significant mechanism of gene regulation in eukaryotes. The crosstalk between RNA methylation and histone modification is critical for chromatin state and gene expression in mammals.
Daolei Zhang +9 more
doaj +1 more source
Detection of 5-methylcytosine in DNA sequences [PDF]
Nucleic Acids Research, 1978 Col E1 DNA has methylated cytosine in the sequence 5'-CC*(A/T)GG-3' and methylated adenine in the sequence 5'-GA*TC-3' at the positions indicated by asterisks(*). When the Maxam-Gilbert DNA sequencing method is applied to this DNA, the methylated cytosine (5-methylcytosine) is found to be less reactive to hydrazine than are cytosine and thymine, so ...
H, Ohmori, J I, Tomizawa, A M, Maxam
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