Results 41 to 50 of about 20,996,854 (289)

Direct enzymatic sequencing of 5-methylcytosine at single-base resolution

Nature Chemical Biology, 2023
Wang et al. developed a bisulfite-free method termed DM-Seq that leverages an unnatural enzyme–substrate pair coupled with a DNA deaminase to sequence 5-methylcytosine at base resolution in sparse DNA samples, circumventing the limitations of chemical ...
Tong Wang, J. M. Fowler, L. Liu, Christian E. Loo, Meiqi Luo, Emily K. Schutsky, Kiara N. Berríos, J. Denizio, Ashley Dvorak, N. Downey, Saira Montermoso, Bianca Pingul, M. Nasrallah, Walraj S. Gosal, Hao Wu, R. Kohli   +15 more
semanticscholar   +1 more source

Levels of DNA cytosine methylation in the Drosophila genome [PDF]

PeerJ, 2018
Insects provide an accessible system to study the contribution of DNA methylation to complex epigenetic phenotypes created to regulate gene expression, chromatin states, imprinting and dosage compensation.
Saniya Deshmukh, VK Chaithanya Ponnaluri, Nan Dai, Sriharsa Pradhan, Deepti Deobagkar   +4 more
doaj   +2 more sources

NSUN2-mediated RNA 5-methylcytosine promotes esophageal squamous cell carcinoma progression via LIN28B-dependent GRB2 mRNA stabilization

Oncogene, 2021
5-Methylcytosine (m5C) is a posttranscriptional RNA modification participating in many critical bioprocesses, but its functions in human cancer remain unclear. Here, by detecting the transcriptome-wide m5C profiling in esophageal squamous cell carcinoma (
Jiachun Su, Guandi Wu, Y. Ye, Jialiang Zhang, Lingxing Zeng, Xudong Huang, Yanfen Zheng, Ruihong Bai, Lisha Zhuang, Mei Li, Ling Pan, Junge Deng, Rui Li, S. Deng, Shaoping Zhang, Zhixiang Zuo, Zexian Liu, Junzhong Lin, D. Lin, Jian Zheng   +19 more
semanticscholar   +1 more source

m5C-Atlas: a comprehensive database for decoding and annotating the 5-methylcytosine (m5C) epitranscriptome

Nucleic Acids Res., 2021
5-Methylcytosine (m5C) is one of the most prevalent covalent modifications on RNA. It is known to regulate a broad variety of RNA functions, including nuclear export, RNA stability and translation. Here, we present m5C-Atlas, a database for comprehensive
Jiongming Ma, B. Song, Zhen Wei, Daiyun Huang, Yu-xin Zhang, Jionglong Su, J. P. D. Magalhães, D. Rigden, Jia Meng, Kunqi Chen   +9 more
semanticscholar   +1 more source

Whole Genome 5′-Methylcytosine Level Quantification in Cirrhotic HCV-Infected Egyptian Patients with and without Hepatocellular Carcinoma

International Journal of Genomics, 2020
DNA methylation is an epigenetic mechanism used by cells to control gene expression. DNA methylation is a commonly used epigenetic signaling tool that can hold genes in the “off” position.
Ahmed M. Awad, Wafaa S. Ragab, Nourhan Degheidy, Said Ahmed Ooda   +3 more
doaj   +1 more source

Tissue distribution of 5-hydroxymethylcytosine and search for active demethylation intermediates. [PDF]

, 2010
5-Hydroxymethylcytosine (hmC) was recently detected as the sixth base in mammalian tissue at so far controversial levels. The function of the modified base is currently unknown, but it is certain that the base is generated from 5-methylcytosine (mC ...
Biel, Martin, Koch Susanne, Brückl, Tobias, Müller, Markus, Michalakis, Stylianos, Globisch Daniel, Martin Münzel, Brückl Tobias, Müller Markus, Münzel, Martin, Martin Biel, Michalakis Stylianos, Wagner Mirko, Markus Müller, Biel Martin, Wagner, Mirko, Tobias Brückl, Carell Thomas, Carell, Thomas, Susanne Koch, Globisch, Daniel, Mirko Wagner, Stylianos Michalakis, Thomas Carell, Daniel Globisch, Münzel Martin, Koch, Susanne   +26 more
core   +1 more source

Tet proteins can convert 5-methylcytosine to 5-formylcytosine and 5-carboxylcytosine

Science, 2011
Evidence for a possible route for DNA demethylation in animals is suggested. 5-methylcytosine (5mC) in DNA plays an important role in gene expression, genomic imprinting, and suppression of transposable elements.
Shinsuke Ito, Li Shen, Qing Dai, Susan C. Wu, L. Collins, J. Swenberg, Chuan He, Yi Zhang   +7 more
semanticscholar   +1 more source

Characterizing 5-methylcytosine in the mammalian epitranscriptome [PDF]

Genome Biology, 2013
The post-transcriptional modification 5-methylcytosine (m5C) occurs in a wide range of coding and non-coding RNAs. We describe transcriptome-wide approaches to capture the global m5C RNA methylome. We also discuss the potential functions of m5C in RNA and compare them to 6-methyladenosine modifications.
Hussain S, Aleksic J, Blanco S, Dietmann S, Frye M.   +4 more
openaire   +2 more sources

Tissue type is a major modifier of the 5-hydroxymethylcytosine content of human genes [PDF]

, 2011
The discovery of substantial amounts of 5-hydroxymethylcytosine (5hmC), formed by the oxidation of 5-methylcytosine (5mC), in various mouse tissues and human embryonic stem (ES) cells has necessitated a reevaluation of our knowledge of 5mC/5hmC patterns ...
Sproul, Duncan, Richard R. Meehan, Katz, Elad,, Nestor, Colm E., Meehan, Richard R,, Elad Katz, Dixon, J Michael,, Harrison, David James, Dixon, J. Michael, Raffaele Ottaviano, Dunican, Donncha, Sproul, Duncan,, Colm E. Nestor, Meehan, Richard R, Dunican, Donncha,, Nestor, Colm,, Reddington, James,, Duncan Sproul, David J. Harrison, Nestor, Colm E, Dunican, Donncha; id_orcid, Harrison, David J, James Reddington, Donncha Dunican, Reddington, James, Reinhardt, Diana,, J. Michael Dixon, Ottaviano, Raffaele,, Katz, Elad, Ottaviano, Raffaele, Dixon, J Michael, Meehan, Richard R., Reinhardt, Diana, Diana Reinhardt, Harrison, David J,   +34 more
core   +1 more source

Total DNA methylation as a biomarker of DNA damage and tumor malignancy in intracranial meningiomas

BMC Cancer, 2020
Background Meningiomas are the most common primary intracranial tumors in adults. They are initially detected with neuroimaging techniques, but definite histological diagnosis requires tumor surgery to collect tumor tissue.
Anna-Maria Barciszewska
doaj   +1 more source