Cell-free DNA methylation as liquid biopsy for the assessment of fibrosis in patients with nonalcoholic steatohepatitis: a gap between innovation and implementation [PDF]
, 2017 The presence of cfDNA in the circulating compartment was demonstrated three decades ago , leading to the emergence of liquid biopsy.However, the implementation of cfDNA methylation as a non-invasive molecular tool for the diagnosis of fibrosis imposes ...
Pirola, Carlos José +1 more
core +1 more source
Bacterial N4-methylcytosine as an epigenetic mark in eukaryotic DNA
Nature Communications, 2022 Eukaryotic DNA can be methylated as 5-methylcytosine and N6-methyladenine, but whether other forms of DNA methylation occur has been controversial. Here the authors show that a bacterial DNA methyltransferase was acquired >60 Mya in bdelloid rotifers ...
Fernando Rodriguez +3 more
doaj +1 more source
5-methylcytosine turnover: Mechanisms and therapeutic implications in cancer
Frontiers in Molecular Biosciences, 2022 DNA methylation at the fifth position of cytosine (5mC) is one of the most studied epigenetic mechanisms essential for the control of gene expression and for many other biological processes including genomic imprinting, X chromosome inactivation and ...
Marion Turpin +3 more
doaj +1 more source
TET2 inhibits differentiation of embryonic stem cells but does not overcome methylation-induced gene silencing [PDF]
, 2014 TET2 is a methylcytosine dioxygenase that is frequently mutated in myeloid malignancies, notably myelodysplasia and acute myeloid leukemia. TET2 catalyses the conversion of 5′-methylcytosine to 5′-hydroxymethylcytosine within DNA and has been implicated ...
Chevassut, Timothy +2 more
core +3 more sources
Oxidative damage to 5-methylcytosine in DNA [PDF]
Nucleic Acids Research, 1995 Exposure of pyrimidines of DNA to ionizing radiation under aerobic conditions or oxidizing agents results in attack on the 5,6 double bond of the pyrimidine ring or on the exocyclic 5-methyl group. The primary product of oxidation of the 5,6 double bond of thymine is thymine glycol, while oxidation of the 5-methyl group yields 5-hydroxymethyluracil ...
S, Zuo, R J, Boorstein, G W, Teebor
openaire +2 more sources
Eicosapentaenoic acid induces DNA demethylation in carcinoma cells through a TET1-dependent mechanism [PDF]
, 2018 In cancer cells, global genomic hypomethylation is found together with localized hypermethylation of CpG islands within the promoters and regulatory regions of silenced tumor suppressor genes.
Billi, Monia +9 more
core +1 more source
Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition
Nature Communications, 2017 Transcription activator-like effector proteins recognise specific DNA sequences via tandem repeats. Here the authors demonstrate TALEs can recognise the methylated bases 5mC and 5hmC, enabling them to detect epigenetic modifications.
Yuan Zhang +7 more
doaj +1 more source
5-Methylcytosine and 5-hydroxymethylcytosine spatiotemporal profiles in the mouse zygote.
PLoS ONE, 2012 BackgroundIn the mouse zygote, DNA methylation patterns are heavily modified, and differ between the maternal and paternal pronucleus. Demethylation of the paternal genome has been described as an active and replication-independent process, although the ...
Juliette Salvaing +5 more
doaj +1 more source
Intronic Non-CG DNA hydroxymethylation and alternative mRNA splicing in honey bees [PDF]
, 2013 Background Previous whole-genome shotgun bisulfite sequencing experiments showed that DNA cytosine methylation in the honey bee (Apis mellifera) is almost exclusively at CG dinucleotides in exons.
Bollig-Fischer, Aliccia +13 more
core +2 more sources
Deamination of 5-Methylcytosine Residues in Mammalian Cells [PDF]
Acta Naturae, 2009 DNA demethylation in mammalia occurs after fertilization and during embryogenesis and accompanies cell aging and cancer transformation. With the help of the primer extension reaction, MALDI MS and DNA cleavage by thymine DNA glycosylase deamination of 5-methylcytosine residues has been shown to take place when the model methylated DNA duplexes are ...
Gromenko, E. +6 more
openaire +4 more sources