Results 101 to 110 of about 2,171 (168)

Direct and bisulfite-free 5-methylcytosine and 5-hydroxymethylcytosine sequencing at single-cell resolution with scTAPS and scCAPS + 

Genome Biology
We present direct sequencing methodologies, scTAPS for 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) and scCAPS + specifically for 5hmC, enabling quantitative detection of 5mC and 5hmC at single-base resolution and single-cell level ...
Xiufei Chen, Jingfei Cheng, Linzhen Kong, Xiao Shu, Haiqi Xu, Masato Inoue, Marion Silvana Fernández-Berrocal, Dagny Sanden Døskeland, Magnar Bjørås, Shivan Sivakumar, Yibin Liu, Jing Ye, Chun-Xiao Song   +12 more
doaj   +1 more source

Cell-Free DNA 5-Hydroxymethylcytosine Signatures for Lung Cancer Prognosis

Cells
Accurate prognostic markers are essential for guiding effective lung cancer treatment strategies. The level of 5-hydroxymethylcytosine (5hmC) in tissue is independently associated with overall survival (OS) in lung cancer patients.
Jianming Shao, Randall J. Olsen, Saro Kasparian, Chuan He, Eric H. Bernicker, Zejuan Li   +5 more
doaj   +1 more source

Intragenic 5hmC correlates with transcription.

, 2016
For (A) P1 and (B) adult mice, 5hmC and input coverages (upper panels; RPKM) and methylation profiles obtained from bisulfite sequencing (lower left panels; in %) were plotted for different sets of genes (< 1 FPKM, ≥ 1 and < 10 FPKM, ≥ 10 and < 50 FPKM, ≥
Thomas G. Nührenberg (758785), David K. Kranzhöfer (3350063), Lutz Hein (69036), Björn A. Grüning (3196326), Ralf Gilsbach (69034), Rolf Backofen (11285)   +5 more
core   +1 more source

JBP1-seq: A fast and efficient method for genome-wide profiling of 5hmC

Genomics, 2014
We developed a novel approach, J-binding protein 1 sequencing (JBP1-seq), that combines the benefits of an improved recombinant JBP1 protein, Nextera-based library construction, and next-generation sequencing (NGS) for genome-wide profiling of 5-hydroxymethylcytosine (5hmC). Compared with the original JBP1, this new recombinant JBP1 was biotinylated in
Cui, Libin, Chung, Tzu Hung, Tan, Darany, Sun, Xueguang, Jia, Xi-Yu   +4 more
openaire   +2 more sources

5hmC was involved in DNA demethylation in fused cells.

, 2015
(A) RT-qPCR analysis of the gene expression of Tet enzymes (mean + s.d ...
Yuxiao Zeng (770249), Ya Qu (770248), Zheng Qin Yin (92567), Haiwei Xu (514432), Wei Wu (27708), Ning Hu (331646), Jun Yang (2084)   +6 more
core   +1 more source

Levels of 5mC and 5hmC in cerebellar genomic DNA.

, 2014
(A) Levels of 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) in genomic DNA isolated from the cerebellum of BTBR T+tf/J and C57BL/6J mice (mean ± SD, n = 5).
Samuil Ivanovsky (665837), S. Jill James (506726), Igor P. Pogribny (665841), Volodymyr Tryndyak (665839), Svitlana Shpyleva (665836), Stepan Melnyk (40890), Aline de Conti (665838), Frederick A. Beland (665840)   +7 more
core   +1 more source

Reduced 5-hydroxymethylcytosine due to TET2 downregulation is associated with chondrosarcoma progression

Scientific Reports
Chondrosarcoma (CS) is the second most common malignant bone tumor, known for its poor prognosis, high recurrence rate, and potential for metastasis. Current prognostic predictive methods rely heavily on histological grading, underscoring the need for ...
Hiroshi Furukawa, Takeshi Iwasaki, Kengo Kawaguchi, Hiroki Sonoda, Kenichi Kohashi, Hidetaka Yamamoto, Yasuharu Nakashima, Yoshinao Oda   +7 more
doaj   +1 more source

Urothelial carcinoma of the bladder shows low 5hmC levels.

, 2016
(A) Normal transitional cell epithelium of the bladder shows strong nuclear 5hmC staining in apical cell layers; basal cell layers exhibit reduced staining intensities (arrowhead). (B) Urothelial carcinoma shows reduced nuclear 5hmC (arrow).
Enrico Munari (750218), Nilda Gonzalez Roibon (846474), Stephania M. Bezerra (843055), Ajay M. Vaghasia (846471), Alcides Chaux (5666497), Diana Taheri (846472), George J. Netto (843066), Sarah Karram (846473), Michael C. Haffner (27579), William G. Nelson (27581), Srinivasan Yegnasubramanian (27577)   +10 more
core   +1 more source

cfDNA hydroxymethylcytosine profiling for detection metastasis and recurrence of Esophageal Squamous Cell Carcinoma

World Journal of Surgical Oncology
Background A blood-based approach to monitor metastasis and recurrence of esophageal squamous cell carcinoma (ESCC) remains undeveloped. This study aimed to establish a dependable model utilizing cfDNA 5-hydroxymethylcytosines (5hmC) signatures to detect
Subinuer Kuerban, Hangyu Chen, Long Chen, Lei Zhang, Xuehui Li, Baixin Zhen, Hong Xiao, Yingzhu Chen, Haitao Zhou, Zhen Liang, Guobing Xu, Yicun Tao, Jian Lin, Xiaozheng Kang   +13 more
doaj   +1 more source

Application of the High-Throughput TAB-Array for the Discovery of Novel 5-Hydroxymethylcytosine Biomarkers in Pancreatic Ductal Adenocarcinoma

Epigenomes, 2019
The clinical outcomes of pancreatic ductal adenocarcinoma (PDAC) remain dismal, with an estimated five-year survival rate of less than 5%. Early detection and prognostic approaches, including robust biomarkers for PDAC, are critical for improving patient
Chang Zeng, Zhou Zhang, Jun Wang, Brian C-H Chiu, Lifang Hou, Wei Zhang   +5 more
doaj   +1 more source