Results 131 to 140 of about 5,986,972 (170)

Roles of Acid Ceramidase and Its Cofactor in Biotherapeutics.

Molecular Pharmaceutics
Polysorbates (PSs) are frequently used as excipients in protein drug formulations. However, recent findings suggest that lipases and esterases can degrade PSs even at very low concentrations, thereby resulting in biotherapeutic instability and particle ...
Sisi Zhang, Christopher Sevinsky, J. Watson, Clair Gameng, Katy Lastowski, Patrick O. Smith, Mark Chiboroski, Emily K Durisin, Svetlana Schussler, Hui Xiao, Ning Li   +10 more
semanticscholar   +3 more sources

Properties of acid ceramidase from human spleen

Lipids and Lipid Metabolism, 1989
We have characterised ceramidase activity in extracts of human spleen from control subjects and from patients with Gaucher disease. In Triton X-100 extracts of control spleens, a broad pH optimum of pH 3.5-5.0 was found; no ceramidase activity was detectable at neutral or alkaline pH.
Joseph M Tager, A W Schram, J M Tager
exaly   +3 more sources

The molecular medicine of acid ceramidase

Biological Chemistry, 2015
Abstract Acid ceramidase (N-acylsphingosine deacylase, EC 3.5.1.23; AC) is the lipid hydrolase responsible for the degradation of ceramide into sphingosine and free fatty acids within lysosomes. The enzymatic activity was first identified over four decades ago and is deficient in two rare inherited disorders, Farber lipogranulomatosis (Farber disease ...
Michael, Frohbergh, Xingxuan, He, Edward H, Schuchman   +2 more
openaire   +2 more sources

Acid and alkaline ceramidases of rat tissues

Biochemistry and Cell Biology, 1986
Ceramidase (N-acylsphingosine deacylase) was measured in rat tissue homogenates with N-[1-14C]oleoyl- and N-[9,10-3H]palmitoyl-sphingosine. Two activities were observed with pH optima of approximately 5 and 8. The activity at pH 8 exceeded that at pH 5 in all tissues and tissue areas examined, and both activities decreased in the following order ...
M W, Spence, S, Beed, H W, Cook
openaire   +2 more sources

Development and validation of an LC-MS/MS method for the determination of ARN14988, an acid ceramidase inhibitor, and its application to a pharmacokinetic study in a mouse model.

Biomedical chromotography, 2023
Despite aggressive treatment approaches, the overall survival of glioblastoma (GBM) patients remained poor with a strong need for more effective chemotherapeutic agents.
Vilashini Rajaratnam, Mohammad Mohiminul Islam, Ethan F Kub, S. Rajaratnam, Kyu Bum Kim, M. T. Rahman, Farjana Rashid, A. Benko, J. Cook, L. Arnold, S. Mirza   +10 more
semanticscholar   +1 more source

Abstract 1585: Targeting acid ceramidase-1 to inhibit sphingolipid metabolism and tumor growth in pancreatic ductal carcinoma

Cancer Research, 2023
Pancreatic ductal adenocarcinoma (PDAC) is a leading cause of cancer-related deaths in the United States. PDAC is an aggressive disease with a poor survival rate of
Krishan Jain, Hindole Ghosh, S. Bhattacharyya, A. Kasi, Ameer Hamza, S. Weir, Michael VanSaun, S. Bossmann, S. Anant, B. Biersack, Prasad Dandawate   +10 more
semanticscholar   +1 more source

Acid ceramidase modulates the lipid profile and exacerbates sensitivity to ferroptosis in senescent cells

Research Square
Cellular senescence, a complex biological process characterized by irreversible cell cycle arrest and the senescence-associated secretory phenotype, has emerged as a critical target for therapeutic development for age-related diseases.
David Soriano-Castell, Marie Goujon, N. J. Dar, A. Currais, P. Maher   +4 more
semanticscholar   +1 more source

Collecting Duct-specific Deletion of Acid Ceramidase Promoted Sodium Retention and Salt-sensitive Hypertension

Physiology
Our previous studies have shown that renal medullary sphingosine-1-phosphate (S1P) stimulates urinary sodium excretion via the S1P type 1 receptor (S1PR1), which primarily located in the collecting ducts, and that knockout of S1PR1 in the collecting ...
Weili Wang, Gaizun Hu, Guangbi Li, Pin-lan Li, Ningjun Li   +4 more
semanticscholar   +1 more source

Involvement of acid ceramidase in the degradation of bioactive N-acylethanolamines

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2021
Bioactive N-acylethanolamines (NAEs) include palmitoylethanolamide, oleoylethanolamide, and anandamide, which exert anti-inflammatory, anorexic, and cannabimimetic actions, respectively. The degradation of NAEs has been attributed to two hydrolases, fatty acid amide hydrolase and NAE acid amidase (NAAA).
Kazuhito, Tsuboi, Tatsuya, Tai, Ryouhei, Yamashita, Hanif, Ali, Takashi, Watanabe, Toru, Uyama, Yoko, Okamoto, Keisuke, Kitakaze, Yasuhiro, Takenouchi, Shinji, Go, Iffat Ara Sonia, Rahman, Hitoshi, Houchi, Tamotsu, Tanaka, Yasuo, Okamoto, Akira, Tokumura, Junko, Matsuda, Natsuo, Ueda   +16 more
openaire   +2 more sources

Loss of Acid Ceramidase in Myeloid Cells Impairs MHC II Expression on Dendritic Cells and Macrophages to Alleviate Chronic Colitis in IL10−/− Mice

Physiology
Inflammatory bowel disease (IBD) is characterized by chronic inflammation in the colon and drastically increases the risk in the development of colorectal cancer (CRC).
Keila S Espinoza, Marilyn T. Marron, Justin M. Snider, Ashley Snider   +3 more
semanticscholar   +1 more source