Results 121 to 130 of about 178 (148)

Functional characterization of CpADF, an actin depolymerizing factor protein in Cryptosporidium parvum

Parasitology Research, 2023
Cryptosporidium is a highly pathogenic water and food-borne zoonotic parasitic protozoan that causes severe diarrhea in humans and animals. Apicomplexan parasites invade host cells via a unique motility process called gliding, which relies on the parasite's microfilaments.
Xiaotian, Zhang, Luyang, Wang, Ruiying, Feng, Guanda, Liang, Wenyan, Hou, Yingying, Zhang, Xiaoying, Li, Longxian, Zhang, Sumei, Zhang   +8 more
openaire   +2 more sources

Regulating filopodial dynamics through actin-depolymerizing factor/cofilin

Anatomical Science International, 2004
The regulation of filopodial dynamics by neurotrophins and other guidance cues plays an integral role in growth cone pathfinding. Filopodia are F-actin-based structures that explore the local environment, generate forces and play a role in growth cone translocation.
Joseph, Fass, Scott, Gehler, Patrick, Sarmiere, Paul, Letourneau, James R, Bamburg   +4 more
openaire   +2 more sources

Analysis of the interactions of actin depolymerizing factor with G- and F-actin

Biochemistry, 1993
Chick actin depolymerizing factor (ADF) is an actin binding protein previously shown to rapidly depolymerize actin filaments in vitro, yielding a 1:1 complex of ADF and actin monomer. Here we show that ADF protects actin monomer from denaturation by EDTA by inhibiting the exchange of actin-bound nucleotide.
S M, Hayden, P S, Miller, A, Brauweiler, J R, Bamburg   +3 more
openaire   +2 more sources

Proteins of the Actin Depolymerizing Factor/Cofilin Family

2008
Actin depolymerizing factor (ADF) and cofilin are the founding members of a group of structurally and functionally related actin binding proteins now collectively known as the ADF/cofilin (AC) family. AC proteins are expressed in all eukaryotic organisms, and their unique ability to bind and dynamize filamentous actin renders them essential to all ...
Janel D. Funk, James R. Bamburg
openaire   +1 more source

The actin-depolymerizing factor destrin has an actin-stabilizing domain

Biochemistry and Cell Biology, 2001
Destrin is a 19 kDa actin-depolymerizing protein of the ADF–cofilin family. Destrin was digested with trypsin to a structurally stable 9.2 kDa fragment that contains the actin-binding sequence. The purified 9.2 kDa fragment has an actin filament stabilizing activity, rather than an actin filament depolymerizing activity. The deleted region is probably
K, Tokuraku, S, Okamoto, M, Katsuki, H, Nakagawa, S, Kotani   +4 more
openaire   +2 more sources

Characterization and expression of an actin-depolymerizing factor from Eimeria tenella

Parasitology Research, 2008
Actin-depolymerizing factor (ADF) plays an important role in remodeling the actin cytoskeleton which contributes much to the invasion of host cells by the apicomplexan parasite. The gene encoding for Eimeria tenella ADF with one intron was cloned and identified by the E.
Jia-Hua, Xu, Zhong-Hua, Qin, Yu-Shen, Liao, Ming-Quan, Xie, An-Xing, Li, Jian-Pin, Cai   +5 more
openaire   +2 more sources

Properties of purified actin depolymerizing factor from chick brain

Biochemistry, 1988
Actin depolymerizing factor (ADF) from 19-day embryonic chick brains has been purified to greater than 98% homogeneity with a yield of 7.2 mg/100 g of brain. Quantitative immunoblotting with a monospecific antibody to ADF indicated that ADF comprises 0.3% of the total brain protein, resulting in an actual purification yield of about 20%.
K A, Giuliano, F A, Khatib, S M, Hayden, E W, Daoud, M E, Adams, D A, Amorese, B W, Bernstein, J R, Bamburg   +7 more
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Cross‐reactivity of antibodies to actin‐ depolymerizing factor/cofilin family proteins and identification of the major epitope recognized by a mammalian actin‐depolymerizing factor/cofilin antibody

ELECTROPHORESIS, 2004
AbstractMembers of the actin‐depolymerizing factor (ADF)/cofilin family of proteins are expressed in all eukaryotic cells. In higher vertebrates, cells often express as many as three different ADF/cofilin genes and each of these proteins may be phosphorylated on serine 3, giving rise to up to six different species.
Alisa E, Shaw, Laurie S, Minamide, Christine L, Bill, Janel D, Funk, Sankar, Maiti, James R, Bamburg   +5 more
openaire   +2 more sources

Identification of two species of actin depolymerizing factor in cultures of BHK cells

Journal of Muscle Research and Cell Motility, 1988
High-speed supernatant obtained from the lysate of cultured BHK cells has been chromatographed on Sepharose-4B, DEAE-cellulose and hydroxyapatite columns, and a fraction has been identified with characteristics similar to an actin depolymerizing factor (ADF), a small protein previously isolated from embryonic chick brain.
A, Koffer, A J, Edgar, J R, Bamburg
openaire   +2 more sources

ADF (Actin Depolymerizing Factor): The Breaker of the Polymer in Homeostasis

2019
This chapter talks about the various isoforms of ADF that exist in different higher eukaryotes and its variation from apicomplexan ADF. We have discussed the structure, the function and the stability of the protein and the way it is regulated in different actin-mediated cellular processes to control the machinery of actin polymerization.
Samridhi Pathak, Ricka Gauba, Sarath Chandra Dantu, Avinash Kale   +3 more
openaire   +1 more source