Results 61 to 70 of about 287,702 (305)

Affimer proteins for F-actin: novel affinity reagents that label F-actin in live and fixed cells [PDF]

, 2018
Imaging the actin cytoskeleton in cells uses a wide range of approaches. Typically, a fluorescent derivative of the small cyclic peptide phalloidin is used to image F-actin in fixed cells. Lifeact and F-tractin are popular for imaging the cytoskeleton in
A Nagasaki, AH Criddle, AJ Spracklen, AM Lengsfeld, B Visegrady, BJ Belin, C Ballestrem, C Ballestrem, C Tiede, C Xie, CW Lee, D Szczesna, E Behrmann, E Wulf, G Lukinavicius, H Aizawa, IN Rybakova, J Ecken von der, J Riedl, J Small, J Wehland, J Wehland, JI Robinson, K Sliogeryte, LD Belmont, M Fritzsche, M Melak, M Nemethova, MA Brehm, MA Michel, MJ Schell, NJ Haywood, SF Wulf, SM Heissler, T Oda, TD Pollard, YL Wang   +36 more
core   +2 more sources

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Organization and Regulation of the Actin Cytoskeleton in the Pollen Tube

Frontiers in Plant Science, 2015
Proper organization of the actin cytoskeleton is crucial for pollen tube growth. However, the precise mechanisms by which the actin cytoskeleton regulates pollen tube growth remain to be further elucidated.
Xiaolu eQu, Yuxiang eJiang, Ming eChang, Xiaonan eLiu, Ruihui eZhang, Shanjin eHuang   +5 more
doaj   +1 more source

The COOH terminus of the c-Abl tyrosine kinase contains distinct F- and G-actin binding domains with bundling activity [PDF]

, 1994
The myristoylated form of c-Abl protein, as well as the P210bcr/abl protein, have been shown by indirect immunofluorescence to associate with F-actin stress fibers in fibroblasts. Analysis of deletion mutants of c-Abl stably expressed in fibroblasts maps
Baltimore, David, Jackson, Peter K., Janmey, Paul A., Matsudaira, Paul T., Sanders, Mitchell C., Van Etten, Richard A.   +5 more
core   +4 more sources

Actin Cable Dynamics and Rho/Rock Orchestrate a Polarized Cytoskeletal Architecture in the Early Steps of Assembling a Stratified Epithelium [PDF]

, 2002
To enable stratification and barrier function, the epidermis must permit self-renewal while maintaining adhesive connections. By generating K14-GFP-actin mice to monitor actin dynamics in cultured primary keratinocytes, we uncovered a role for the actin ...
Bauer, Christoph, Fuchs, Elaine, Vaezi, Alec, Vasioukhin, Valeri   +3 more
core   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Schip1 is a novel podocyte foot process protein that mediates actin cytoskeleton rearrangements and forms a complex with Nherf2 and ezrin. [PDF]

PLoS ONE, 2015
Podocyte foot process effacement accompanied by actin cytoskeleton rearrangements is a cardinal feature of many progressive human proteinuric diseases.By microarray profiling of mouse glomerulus, SCHIP1 emerged as one of the most highly enriched ...
Ljubica Perisic, Patricia Q Rodriguez, Kjell Hultenby, Ying Sun, Mark Lal, Christer Betsholtz, Mathias Uhlén, Annika Wernerson, Ulf Hedin, Timo Pikkarainen, Karl Tryggvason, Jaakko Patrakka   +11 more
doaj   +1 more source

The Bacterial Actin-Like Cytoskeleton [PDF]

Microbiology and Molecular Biology Reviews, 2006
SUMMARYRecent advances have shown conclusively that bacterial cells possess distant but true homologues of actin (MreB, ParM, and the recently uncovered MamK protein). Despite weak amino acid sequence similarity, MreB and ParM exhibit high structural homology to actin.
openaire   +4 more sources

Understanding and measuring mechanical signals in the tumor stroma

FEBS Open Bio, EarlyView.
This review discusses cancer‐associated fibroblast subtypes and their functions, particularly in relation to extracellular matrix production, as well as the development of 3D models to study tumor stroma mechanics in vitro. Several quantitative techniques to measure tissue mechanical properties are also described, to emphasize the diagnostic and ...
Fàtima de la Jara Ortiz, Chiara Cimmino, Maurizio Ventre, Alessandra Cambi   +3 more
wiley   +1 more source

Density-dependent flow generation in active cytoskeletal fluids [PDF]

Scientific Reports 14, 31339 (2024)
The actomyosin cytoskeleton, a protein assembly comprising actin fibers and the myosin molecular motor, drives various cellular dynamics through contractile force generation at high densities. However, the relationship between the density dependence of the actomyosin cytoskeleton and force-controlled ordered structure remains poorly understood. In this
arxiv   +1 more source