Results 41 to 50 of about 31,296 (289)

Rapid production of pure recombinant actin isoforms in Pichia pastoris [PDF]

, 2018
Actins are major eukaryotic cytoskeletal proteins, which perform many important cell functions, including cell division, cell polarity, wound healing, and muscle contraction.
Alioto, Salvatore, Balasubramanian, Mohan K., Chapa y Lazo, Bernardo, Clarke, Scott T., Goode, Bruce L., Hatano, Tomoyuki, Hernandez Fernaud, Juan Ramon, Jones, Alexandra M., Kamnev, Anton, McAinsh, Andrew D., Mishima, Masanori, Palani, Saravanan, Robinson, Robert C., Roscioli, Emanuele, Sampath, Karuna, Sivashanmugam, Lavanya   +15 more
core   +1 more source

The mother of all actins?

eLife, 2016
New insights into the structure of filaments made of crenactin, a homolog of actin found in archaea, shed light on how the cytoskeleton might have evolved.
Felipe Merino, Stefan Raunser
doaj   +1 more source

A method for rapidly screening functionality of actin mutants and tagged actins [PDF]

Biological Procedures Online, 2004
Recombinant production and biochemical analysis of actin mutants has been hampered by the fact that actin has an absolute requirement for the eukaryotic chaperone CCT to reach its native state.
Rommelaere Heidi, Waterschoot Davy, Neirynck Katrien, Vandekerckhove Joël, Ampe Christophe   +4 more
doaj   +1 more source

Concanavalin A-induced cap formation in rat ascites hepatoma cells (AH 7974) and the interaction of cytoplasmic proteins with plasma membranes. [PDF]

, 1987
Concanavalin A (Con A) induced cap formation in rat ascites hepatoma cells (AH7974). In these Con A-treated cells, the association of cytoplasmic proteins with cell membranes was suggested by observing their Triton shells.
Moromizato, Yasunori, Sasaki, Junzo, Watanabe, Sadahiro   +2 more
core   +1 more source

A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

Nanotechnology and Precision Engineering, 2020
As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive.
Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Shuang Zhang, Junbo Wang, Jian Chen   +6 more
doaj   +1 more source

Orchestrated control of filaggrin-actin scaffolds underpins cornification. [PDF]

, 2018
Epidermal stratification critically depends on keratinocyte differentiation and programmed death by cornification, leading to formation of a protective skin barrier.
Bernardino de la Serna, Jorge, Colin-York, Huw, Eggeling, Christian, Fritzsche, Marco, Gutowska-Owsiak, Danuta, Leeming, Michael, Naeem, Aishath, Ogg, Graham, O’Shaughnessy, Ryan, Podobas, Ewa Izabela   +9 more
core   +6 more sources

Pathogenic Germline PALB2 and RAD50 Variants in Patients With Relapsed Ewing Sarcoma

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Approximately 10% of patients with Ewing sarcoma (EwS) have pathogenic germline variants. Here, we report two cases: first, a novel germline pathogenic variant in partner and localizer of BRCA2 (PALB2) in a patient with a late EwS relapse. Its impact on homologous recombination is demonstrated, and breast cancer risk is discussed.
Molly Mack, A. Carolina Tufino, Mohd Azrin Jamalruddin, Sreya Dey, Yinghong Pan, Elina Mukherjee, Jessica D. Daley, Julia Meade, Mahmoud Aarabi, Lisa Maurer, James Cooper, Kara A. Bernstein, Kelly M. Bailey   +12 more
wiley   +1 more source

TNIK Regulates Cytoskeletal Organization to Promote Focal Adhesion Turnover and Mitosis in Lung Adenocarcinoma

Frontiers in Bioscience-Landmark
Background: Lung cancer is the primary cause of cancer-related mortality, but the molecular mechanisms behind this malignancy remain unclear.
Yao Li, Meng-yao Song, Xing Hu, Xue-hua Sun, Tao Zhang, Lu Zhang, Ying-xiong Wang, Qian Zhang, Chun-dong Zhang, Lian Zhang   +9 more
doaj   +1 more source

Partially Redundant Actin Genes in Chlamydomonas Control Transition Zone Organization and Flagellum-Directed Traffic

Cell Reports, 2019
Summary: The unicellular green alga Chlamydomonas reinhardtii is a biflagellated cell with two actin genes: one encoding a conventional actin (IDA5) and the other encoding a divergent novel actin-like protein (NAP1).
Brittany Jack, David M. Mueller, Ann C. Fee, Ashley L. Tetlow, Prachee Avasthi   +4 more
doaj   +1 more source

The intron-containing gene for yeast profilin (PFY) encodes a vital function [PDF]

, 1988
The gene coding for profilin (PFY), an actin-binding protein, occurs as a single copy in the haploid genome of Saccharomyces cerevisiae and is required for spore germination and cell viability.
Bandlow, Wolfhard, Magdolen, Viktor, Müller, Günter, Oechsner, Ulrich   +3 more
core   +2 more sources