Results 91 to 100 of about 2,930,787 (173)

Necrostatin-1 Attenuates Trauma-Induced Mouse Osteoarthritis and IL-1β Induced Apoptosis via HMGB1/TLR4/SDF-1 in Primary Mouse Chondrocytes

Frontiers in Pharmacology, 2018
Necrostatin-1 (Nec-1) is a specific small molecule inhibitor of receptor-interacting protein kinase 1 (RIPK1) that specifically inhibits phosphorylation of RIPK1. RIPK1 regulates inflammation and cell death by interacting with receptor-interacting serine/
Shuang Liang, Zheng-tao Lv, Zheng-tao Lv, Jia-ming Zhang, Yu-ting Wang, Yong-hui Dong, Zheng-gang Wang, Kun Chen, Kun Chen, Peng Cheng, Qing Yang, Feng-jing Guo, Wei-wei Lu, Wen-tao Zhu, An-min Chen   +14 more
doaj   +1 more source

Co-culture of hMSCs transduced with ADAMTS5-targeting shRNA effectively reduces ADAMTS5 expression in flow cytometry sorted RFP-labeled SW982.

, 2015
(A) Following co-culture of non-targeting control shRNA or ADAMTS5-targeting shRNA transduced hMSCs with RFP labeled SW982 cells, the cells were sorted by flow cytometry.
Shuo Liu (108276), Joseph P. Stains (755219), Eugene Y. Koh (755218), Corinne Niger (755217)   +3 more
core   +1 more source

Co-culture of hMSCs transduced with ADAMTS5-targeting shRNA can effectively knockdown the expression of ADAMTS5 in SW982 cells.

, 2015
(A), A western blot of whole cell extracts from SW982 cells or hMSCs in mono-culture probed with anti-ADAMTS5 or anti-GAPDH antibodies, as indicated, shows that ADAMTS5 is most abundantly expressed in SW982 cells, although a modest amount is also ...
Shuo Liu (108276), Joseph P. Stains (755219), Eugene Y. Koh (755218), Corinne Niger (755217)   +3 more
core   +1 more source

ADAMTS5 Is a Biomarker for Prediction of Response to Infliximab in Patients with Rheumatoid Arthritis

, 2010
Objective.To identify a biomarker for prediction of the response to infliximab (IFX) in patients with rheumatoid arthritis (RA), we focused on a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) that seems to play a key role in ...
YUKA ITAMI, TETSUO MORISHITA, TSUTOMU TAKEUCHI, NAOSHI SHINOZAKI, KENSEI TSUZAKA   +4 more
core   +1 more source

Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp. [PDF]

, 2012
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D., Stefan G D Rüdiger, Maurice, Madelon M., David P Minde, Sub Cellular Protein Chemistry, Stefan G. D. Rüdiger, Madelon M Maurice, Minde, David P., Cellular Protein Chemistry, David P. Minde, Madelon M. Maurice   +10 more
core   +3 more sources

Role Of Gpx6 In TMJ OA Cartilage Damage

International Dental Journal
Aim or purpose: This study explores GPx6′s function in TMJ OA and its therapeutic potential. Materials and methods: This study was approved by the Medical Ethics Committee.
Wenfang Wang
doaj   +1 more source

Analysis of Disulfide Bond Formation [PDF]

, 2017
In this unit, protocols are provided for detection of disulfide bond formation in cultures of intact cells and in an in vitro translation system containing isolated microsomes or semi-permeabilized cells.
Sub Cellular Protein Chemistry, Ineke Braakman, Hebert, Daniel N., Lydia Lamriben, Guus van Zadelhoff, van Zadelhoff, Guus, Braakman, Ineke, Daniel N. Hebert, Lamriben, Lydia, Cellular Protein Chemistry   +9 more
core   +2 more sources

YAP1 Knockdown Reduces IL-1β-Induced Human Chondrocyte Inflammation and Promotes Human MSC Chondrogenesis

Pharmaceuticals
Background: Yes-associated protein 1 (YAP1), a key effector of the Hippo signaling pathway and mechanosensitive transcriptional coactivator, plays a complex role in osteoarthritis (OA) and cartilage regeneration.
Liru Wen, Sibylle Grad, Laura B. Creemers, Martin J. Stoddart   +3 more
doaj   +1 more source

A Damaging COL6A3 Variant Alters the MIR31HG‐Regulated Response of Chondrocytes in Neocartilage Organoids to Hyperphysiologic Mechanical Loading

Advanced Science
The pericellular matrix (PCM), with its hallmark proteins collagen type VI (COLVI) and fibronectin (FN), surrounds chondrocytes and is critical in transducing the biomechanical cues.
Niek GC Bloks, Zainab Harissa, Giorgia Mazzini, Shaunak S Adkar, Amanda R Dicks, Ghazaleh Hajmousa, Nancy Steward, Roman I. Koning, Aat Mulder, Berend B.R. deKoning, Margreet Kloppenburg, Rodrigo Coutinho deAlmeida, Yolande FM Ramos, Farshid Guilak, Ingrid Meulenbelt   +14 more
doaj   +1 more source

The Regulatory Effects and Mechanisms of Piezo1 Channel on Chondrocytes and Bone Metabolic Dysregulation in Osteoarthritis

Shengwu huaxue yu shengwu wuli jinzhan
Osteoarthritis (OA), a highly prevalent degenerative joint disease worldwide, is defined by articular cartilage degradation, abnormal bone remodeling, and persistent chronic inflammation.
LI Yan, LIU Tao, GU Yu-Biao, TIAN Hui-Qing, ZHANG Lei, BAI Bi-Hui, HE Zhi-Jun, CHEN Wen, LI Jin-Peng, LI Fei   +9 more
doaj   +1 more source