Results 51 to 60 of about 2,414,823 (352)

Epidemiology of Dog and Cat Abandonment in Spain (2008–2013)

open access: yesAnimals, 2015
Millions of pets are abandoned worldwide every year, which is an important animal welfare and financial problem. This paper was divided into three studies.
Jaume Fatjó   +6 more
doaj   +1 more source

Noncovalent Adsorption of Single-Stranded and Double-Stranded DNA on the Surface of Gold Nanoparticles

open access: yesApplied Sciences, 2023
Understanding the patterns of noncovalent adsorption of double-stranded nucleic acids (dsDNA) on gold nanoparticles (GNPs) was the aim of this study. It was found that the high-affinity motifs in DNA can and do act as an “anchor” for the fixation of the ...
Ekaterina A. Gorbunova   +3 more
doaj   +1 more source

Affinity Weighted Embedding [PDF]

open access: yes, 2013
Supervised (linear) embedding models like Wsabie and PSI have proven successful at ranking, recommendation and annotation tasks. However, despite being scalable to large datasets they do not take full advantage of the extra data due to their linear ...
Weiss, Ron, Weston, Jason, Yee, Hector
core  

Clustering via kernel decomposition [PDF]

open access: yes, 2006
Spectral clustering methods were proposed recently which rely on the eigenvalue decomposition of an affinity matrix. In this letter, the affinity matrix is created from the elements of a nonparametric density estimator and then decomposed to obtain ...
Girolami, M.   +2 more
core   +2 more sources

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

open access: yesFEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Current applications of antibody microarrays

open access: yesClinical Proteomics, 2018
The concept of antibody microarrays is one of the most versatile approaches within multiplexed immunoassay technologies. These types of arrays have increasingly become an attractive tool for the exploratory detection and study of protein abundance ...
Ziqing Chen   +3 more
doaj   +1 more source

Impact of peptide:HLA complex stability for the identification of SARS-CoV-2-specific CD8+T cells

open access: yesFrontiers in Immunology, 2023
Induction of a lasting protective immune response is dependent on presentation of epitopes to patrolling T cells through the HLA complex. While peptide:HLA (pHLA) complex affinity alone is widely exploited for epitope selection, we demonstrate that ...
Olivia Lie-Andersen   +13 more
doaj   +1 more source

Effect of thiols on beta 2-adrenoceptors in human mononuclear leucocytes [PDF]

open access: yes, 1988
The effect of the disulfide reducing agent dithiothreitol (DTT) and other thiols on binding of the beta-adrenoceptor antagonist (-)-125iodocyanopindolol (125ICYP) to human mononuclear leucocytes (MNL) was investigated.
Anhäupl, T.   +3 more
core  

Structural insights into lacto‐N‐biose I recognition by a family 32 carbohydrate‐binding module from Bifidobacterium bifidum

open access: yesFEBS Letters, EarlyView.
Bifidobacterium bifidum establishes symbiosis with infants by metabolizing lacto‐N‐biose I (LNB) from human milk oligosaccharides (HMOs). The extracellular multidomain enzyme LnbB drives this process, releasing LNB via its catalytic glycoside hydrolase family 20 (GH20) lacto‐N‐biosidase domain.
Xinzhe Zhang   +5 more
wiley   +1 more source

Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes

open access: yesFrontiers in Molecular Biosciences, 2020
CRISPR-Cas systems employ ribonucleoprotein complexes to identify nucleic acid targets with complementarity to bound CRISPR RNAs. Analyses of the high diversification of these effector complexes suggest that they can exhibit a wide spectrum of target ...
Hanna Müller-Esparza   +8 more
doaj   +1 more source

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