Results 11 to 20 of about 733,038 (191)

Engaging an HIV vaccine target through the acquisition of low B cell affinity

Nature Communications, 2023
Low affinity is common for germline B cell receptors (BCR) seeding development of broadly neutralizing antibodies (bnAbs) that engage hypervariable viruses, including HIV. Antibody affinity selection is also non-homogenizing, insuring the survival of low
Larance Ronsard, Ashraf S. Yousif, Faez Amokrane Nait Mohamed, Jared Feldman, Vintus Okonkwo, Caitlin McCarthy, Julia Schnabel, Timothy Caradonna, Ralston M. Barnes, Daniel Rohrer, Nils Lonberg, Aaron Schmidt, Daniel Lingwood   +12 more
doaj   +1 more source

Synthesis, in vitro, and in vivo biological evaluation and molecular docking simulations of chiral alcohol and ether derivatives of the 1,5-diarylpyrrole scaffold as novel anti-inflammatory and analgesic agents. [PDF]

, 2008
Following our previous research on anti-inflammatory drugs (NSAIDs), we report here the synthesis of chiral 1,5-diarylpyrroles derivatives that were characterized for their in vitro inhibitory effects toward cyclooxygenase (COX) isozymes.
ANZELLOTTI P., ANZINI M., BIAVA M., BOTTA M., CIRILLI R., FERRETTI R., FORLI ., GALLINELLA B., GHELARDINI C., GIORDANI A., LA TORRE F., MAKOVEC F., MANETTI F., NORCINI M., PATRIGNANI P., PERGOLA C., POCE G., PORRETTA G. C., ROSSI, ANTONIETTA, SAUTEBIN, LIDIA, SUPINO S.   +20 more
core   +1 more source

Generating Recombinant Antibodies to Membrane Proteins through Phage Display

Antibodies, 2016
One of the most important classes of proteins in terms of drug targets is cell surface membrane proteins, and yet it is a challenging set of proteins for generating high-quality affinity reagents.
Renhua Huang, Margaret M. Kiss, Melissa Batonick, Michael P. Weiner, Brian K. Kay   +4 more
doaj   +1 more source

Evolutionary fine-tuning of conformational ensembles in FimH during host-pathogen interactions [PDF]

, 2017
Positive selection in the two-domain type 1 pilus adhesin FimH enhances Escherichia coli fitness in urinary tract infection (UTI). We report a comprehensive atomic-level view of FimH in two-state conformational ensembles in solution, composed of one low ...
Dodson, Karen W, Gross, Michael L, Hannan, Thomas J, Hibbing, Michael E, Holehouse, Alex S, Hultgren, Scott J, Janetka, James, Kalas, Vasilios, Pappu, Rohit V, Pinkner, Jerome S, Tolia, Niraj H, Zhang, Hao   +11 more
core   +3 more sources

Strategies for Selecting Membrane Protein-Specific Antibodies using Phage Display with Cell-Based Panning

Antibodies, 2017
Membrane proteins are attractive targets for monoclonal antibody (mAb) discovery and development. Although several approved mAbs against membrane proteins have been isolated from phage antibody libraries, the process is challenging, as it requires the ...
Mohamed A. Alfaleh, Martina L. Jones, Christopher B. Howard, Stephen M. Mahler   +3 more
doaj   +1 more source

Carbon nanotube decorated magnetic microspheres as an affinity matrix for biomolecules [PDF]

, 2013
Carbon nanotube (CNT) decorated magnetic microspheres were fabricated to develop a multimodal platform that utilizes non-covalent molecular interactions of CNTs to magnetically separate biomolecules.
Kolkar Mohammed, Javed Hussain Niazi, Ünal, Hayriye   +1 more
core   +1 more source

A systematic evolution of ligands by exponential enrichment workflow with consolidated counterselection to efficiently isolate high‐affinity aptamers

Engineering Reports, 2020
Efficient selection of nucleic acid aptamers with high affinity and specificity for a broad range of targets remains challenging. Historically, aptamer selections have been protracted and tedious processes, often requiring double‐digit rounds of ...
Jonah C. Rosch, Daniel A. Balikov, Franklin Gong, Ethan S. Lippmann   +3 more
doaj   +1 more source

Representations of Affine Multifunctions by Affine Selections [PDF]

Set-Valued Analysis, 2008
zbMATH Open Web Interface contents unavailable due to conflicting licenses.
openaire   +2 more sources

Selection of functional human antibodies from retroviral display libraries [PDF]

, 2004
Antibody library technology represents a powerful tool for the discovery and design of antibodies with high affinity and specificity for their targets.
Urban, Johannes H., Schneider, Richard M., Compte, Marta, Finger, Carsten, Cichutek, Klaus, Álvarez-Vallina, Luis, Buchholz, Christian   +6 more
core   +2 more sources

Novel MHC-Independent αβTCRs Specific for CD48, CD102, and CD155 Self-Proteins and Their Selection in the Thymus

Frontiers in Immunology, 2020
MHC-independent αβTCRs (TCRs) recognize conformational epitopes on native self-proteins and arise in mice lacking both MHC and CD4/CD8 coreceptor proteins. Although naturally generated in the thymus, these TCRs resemble re-engineered therapeutic chimeric
François Van Laethem, Ingrid Saba, Jinghua Lu, Abhisek Bhattacharya, Xuguang Tai, Terry I. Guinter, Britta Engelhardt, Amala Alag, Mirelle Rojano, Jennifer M. Ashe, Ken-ichi Hanada, James C. Yang, Peter D. Sun, Alfred Singer   +13 more
doaj   +1 more source