Functional Characterization of Small Alarmone Synthetase and Small Alarmone Hydrolase Proteins from Treponema denticola [PDF]
Microbiology Spectrum, 2023 The stringent response enables bacteria to survive nutrient starvation, antibiotic challenge, and other threats to cellular survival. Two alarmone (magic spot) second messengers, guanosine pentaphosphate (pppGpp) and guanosine tetraphosphate (ppGpp ...
Miao Wang +3 more
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How to quantify magic spots - a brief overview of (p)ppGpp detection and quantitation methods [PDF]
Frontiers in Molecular BiosciencesGuanosine tetra- and penta-phosphates, collectively known as (p)ppGpp, are well-known second messengers of cellular stress responses in bacteria and plants.
Filip Gąsior +2 more
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Bacteria export alarmone synthetases that produce (p)ppApp and (p)ppGpp [PDF]
mBioGuanosine penta- and tetraphosphate [(p)ppGpp] and their adenosine analogs [(p)ppApp] are bacterial second messengers known as alarmones. Members of the RelA-SpoT homolog (RSH) family synthesize (p)ppGpp to mediate the stringent response during nutrient ...
Shehryar Ahmad +10 more
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Metabolic Promiscuity of an Orphan Small Alarmone Hydrolase Facilitates Bacterial Environmental Adaptation [PDF]
mBio, 2022 Small alarmone hydrolases (SAHs) are alarmone metabolizing enzymes found in both metazoans and bacteria. In metazoans, the SAH homolog Mesh1 is reported to function in cofactor metabolism by hydrolyzing NADPH to NADH.
Danny K. Fung +7 more
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Nucleotides as Bacterial Second Messengers [PDF]
Molecules, 2023 In addition to comprising monomers of nucleic acids, nucleotides have signaling functions and act as second messengers in both prokaryotic and eukaryotic cells. The most common example is cyclic AMP (cAMP).
Mario E. Cancino-Diaz +3 more
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Stringent Response in Mycobacteria: From Biology to Therapeutic Potential [PDF]
Pathogens, 2021 Mycobacterium tuberculosis is a human pathogen that can thrive inside the host immune cells for several years and cause tuberculosis. This is due to the propensity of M.
Kuldeepkumar Ramnaresh Gupta +3 more
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Antibiotic Resistance to Mycobacterium tuberculosis and Potential Use of Natural and Biological Products as Alternative Anti-Mycobacterial Agents [PDF]
Antibiotics, 2022 Background: Tuberculosis (TB) is an infectious disease caused by the bacillus Mycobacterium tuberculosis (Mtb). TB treatment is based on the administration of three major antibiotics: isoniazid, rifampicin, and pyrazinamide. However, multi-drug resistant
Roberto Arrigoni +5 more
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Controlled Delivery and Light-Induced Release of Magic Spot Nucleotides in Escherichia coli. [PDF]
Angew Chem Int Ed EnglWe synthesized photocaged, clickable and isotope‐labeled “magic spot” Nucleotides and delivered them into Escherichia coli comparing different approaches. Light‐controlled release inside living cells enabled tracking of their conversion from pppGpp to ppGpp by capillary electrophoresis mass spectrometry and revealed their impact on the growth rate of a
Popp C +12 more
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The alarmones (p)ppGpp are part of the heat shock response of Bacillus subtilis
PLoS Genetics, 2020 Bacillus subtilis cells are well suited to study how bacteria sense and adapt to proteotoxic stress such as heat, since temperature fluctuations are a major challenge to soil-dwelling bacteria. Here, we show that the alarmones (p)ppGpp, well known second
Christian Frese +2 more
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Stress-induced ribosome degradation in Bacillus subtilis is mediated by the RNase Y-specificity complex [PDF]
Nature CommunicationsLimiting ribosome synthesis and activity is crucial for adaptation to stresses, such as heat or nutrient starvation. In Bacillus subtilis, this can be achieved through the coordinated action of the alarmones (p)ppGpp and the transcription factor Spx ...
Fabián A. Cornejo +12 more
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