Results 21 to 30 of about 748 (149)
NirD curtails the stringent response by inhibiting RelA activity in Escherichia coli
eLife, 2021 Bacteria regulate their metabolism to adapt and survive adverse conditions, in particular to stressful downshifts in nutrient availability. These shifts trigger the so-called stringent response, coordinated by the signaling molecules guanosine tetra and ...
Loïc Léger +4 more
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Microorganisms, 2020 Biofilm infections have no approved effective medical treatments and can only be disrupted via physical means. This means that any biofilm infection that is not addressable surgically can never be eliminated and can only be managed as a chronic disease ...
Donald C. Hall +4 more
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Nature Communications, 2021 Diadenosine polyphosphates (ApAs) are involved in cellular stress signaling but only a few molecular targets have been characterized so far. Here, the authors develop Ap n A-based photoaffinity-labeling probes and use them to identify Ap3A and Ap4A ...
Lena Krüger +10 more
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The expanding field of non-canonical RNA capping: new enzymes and mechanisms
Royal Society Open Science, 2021 Recent years witnessed the discovery of ubiquitous and diverse 5′-end RNA cap-like modifications in prokaryotes as well as in eukaryotes. These non-canonical caps include metabolic cofactors, such as NAD+/NADH, FAD, cell wall precursors UDP-GlcNAc ...
Jana Wiedermannová +2 more
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Structural Basis for Transcription Regulation by Alarmone ppGpp [PDF]
Cell, 2004 Guanosine-tetraphosphate (ppGpp) is a major regulator of stringent control, an adaptive response of bacteria to amino acid starvation. The 2.7 A resolution structure of the Thermus thermophilus RNA polymerase (RNAP) holoenzyme in complex with ppGpp reveals that ppGpp binds to the same site near the active center in both independent RNAP molecules in ...
Artsimovitch, Irina +7 more
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Regulation of ς factor competition by the alarmone ppGpp [PDF]
Genes & Development, 2002 Many regulons controlled by alternative ς factors, including ς S and ς 32 , are poorly induced in cells lacking the alarmone ppGpp. We show that ppGpp is not absolutely required for the activity of ς S -dependent ...
Miki, Jishage +3 more
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Molecular basis for noncanonical transcription initiation from Np<sub>4</sub>A alarmones. [PDF]
Nat Chem BiolStress-induced dinucleoside tetraphosphates (Np4Ns, where N is adenosine, guanosine, cytosine or uridine) are ubiquitous in living organisms, yet their function has been largely elusive for over 50 years. Recent studies have revealed that RNA polymerase can influence the cellular lifetime of transcripts by incorporating these alarmones into RNA as 5 ...
Duan W +12 more
europepmc +3 more sources
Diverse molecular mechanisms of transcription regulation by the bacterial alarmone ppGpp [PDF]
Molecular Microbiology, 2021 AbstractBacteria must rapidly detect and respond to stressful environmental conditions. Guanosine tetraphosphate (ppGpp) is a universal stress signal that, in most bacteria, drives the reprograming of transcription at a global level. However, recent studies have revealed that the molecular mechanisms utilized by ppGpp to rewire bacterial transcriptomes
Brady A. Travis, Maria A. Schumacher
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Functional Characterization of a Small Alarmone Hydrolase in Corynebacterium glutamicum [PDF]
Frontiers in Microbiology, 2018 The (pp)pGpp metabolism is an important component of bacterial physiology as it is involved in various stress responses and mechanisms of cell homeostasis, e.g., the regulation of growth. However, in order to better understand the (pp)pGpp associated regulation, it is crucial to study the molecular mechanisms of (pp)pGpp metabolism.
Ruwe, Matthias +3 more
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Dynamic Metabolic Response to (p)ppGpp Accumulation in Pseudomonas putida
Frontiers in Microbiology, 2022 The stringent response is a ubiquitous bacterial reaction triggered by nutrient deprivation and mediated by the intracellular concentrations of ppGpp and pppGpp.
Philippe Vogeleer, Fabien Létisse
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