Results 51 to 60 of about 8,038 (247)

Inhibition of KDEL Receptors Remodels the Tumor Microenvironment for T Cell Independent Tumor Regression

Advanced Science, EarlyView.
Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak, Hong Wang, Belinda Willard, Timothy A. Chan, William C Merrick, Zheng‐Rong Lu, Boaz Tirosh   +6 more
wiley   +1 more source

Triose phosphate isomerase from the coelacanth. An approach to the rapid determination of an amino acid sequence with small amounts of material [PDF]

Biochemical Journal, 1974
The preparation and purification of cyanogen bromide fragments from [14C]carboxymethylated coelacanth triose phosphate isomerase is presented. The automated sequencing of these fragments, the lysine-blocked tryptic peptides derived from them, and also of the intact protein, is described.
E, Kolb, J I, Harris, J, Bridgen
openaire   +2 more sources

A Single‐Cell Transcriptomic Atlas of the Ovine Rumen Microbiome Characterizes Lineage‐Specific Metabolic Shifts Associated with Host Heat Tolerance

Advanced Science, EarlyView.
An optimized single‐cell transcriptomic framework profiles over 60 000 cells to map the ovine rumen microbiome, partitioning the ecosystem into seven cross‐species functional clusters. In heat‐resistant hosts, a lineage‐specific metabolic shift in Anaerovibrio lipolyticus toward a highly glycolytic phenotype contributes to a “nutritional sparing ...
Sanbao Zhang, Qinyang Jiang, Junjie Ma, Jianwei Zou, Fan Wang, Feifei Lv, Yanna Huang, Yongcheng Wang, Ziye Xu   +8 more
wiley   +1 more source

s-cyclophilin is retained intracellularly via a unique COOH-terminal sequence and colocalizes with the calcium storage protein calreticulin [PDF]

, 1992
Cyclophilins (cyclosporin A-binding proteins) are conserved, ubiquitous, and abundant proteins that accelerate the isomerization of XaaPro peptide bonds and the refolding of proteins in vitro.
Arber, S., P Caroni, Krause, Karl-Heinz, K H Krause, S Arber, Caroni, P., Krause, K. H.   +6 more
core   +1 more source

Enzyme Architecture: Amino Acid Side-Chains That Function To Optimize the Basicity of the Active Site Glutamate of Triosephosphate Isomerase [PDF]

Journal of the American Chemical Society, 2018
We report pH rate profiles for kcat and Km for the isomerization reaction of glyceraldehyde 3-phosphate catalyzed by wildtype triosephosphate isomerase (TIM) from three organisms and by ten mutants of TIM; and, for Ki for inhibition of this reaction by phosphoglycolate trianion (I3-).
Xiang Zhai, Christopher J. Reinhardt, M. Merced Malabanan, Tina L. Amyes, John P. Richard   +4 more
openaire   +2 more sources

AS‐pHopt: An Optimal pH Prediction Model Enhanced by Active Site of Enzymes

Advanced Intelligent Discovery, EarlyView.
To address the low accuracy of enzyme optimal pH (pHopt) prediction, this study develops active site‐based pHopt (AS‐pHopt), a prediction model enhanced by active site information and pseudo‐label prediction. Integrating key structural and physicochemical features affecting enzyme pHopt, AS‐pHopt uses Evolutionary Scale Modeling (ESM)‐2 with active ...
Wenxiang Song, Xinmin Li, Hongbo Yu, Le Xiong, Guixia Liu, Weihua Li, Yun Tang   +6 more
wiley   +1 more source

Identification of Amino Acids that Account for Long-Range Interactions in Two Triosephosphate Isomerases from Pathogenic Trypanosomes

PLoS ONE, 2011
من أجل فهم أفضل للعلاقة بين البنية والوظيفة في البروتينات، من الضروري تحديد الأحماض الأمينية ذات الصلة بوظائف البروتين القابلة للقياس. بسبب الاتصالات العديدة التي تنشئها الأحماض الأمينية داخل البروتينات والطبيعة التعاونية لتفاعلاتها، من الصعب تحقيق هذا الهدف.
Itzhel García‐Torres, Nallely Cabrera, Alfredo Torres‐Larios, Mónica Rodríguez-Bolaños, Selma Díaz-Mazariegos, Armando Gómez‐Puyou, Ruy Pérez‐Montfort   +6 more
openaire   +4 more sources

Isolation and characterisation of cDNAs encoding protein disulphide isomerases and cyclophilins in wheat

, 2001
The enzyme families of protein disulphide isomerases (PDI) and cyclophilins catalyse the isomerisation of disulphide bonds and the rotation of Xaa-Pro peptide bonds in nascent proteins, respectively, making them excellent candidates for regulating the ...
Johnson, Joshua C, Clarke, Bryan C, Johnson, Joshua, Clarke, B. C, Bhave, Mrinal   +4 more
core   +1 more source

Enantioselective Synthesis of a Tricyclic, sp3-Rich Diazatetradecanedione: an Amino Acid-Based Natural Product- Like Scaffold

, 2020
6‐, 7‐, and 8‐membered rings are assembled from a linear precursor by successive cyclisation reactions to construct a tricyclic diazatricyclo[6.5.1.0⁴,⁹]‐tetradecanedione scaffold. Advanced building blocks based on d‐aspartic acid and l‐pyroglutamic acid
Mayer, Peter, Hausch, F., Christian Meyners, Matthias Bischoff, Peter Mayer, Meyners, Christian, Mayer, P., Hausch, Felix, Felix Hausch, Bischoff, Matthias, Meyners, C., Bischoff, M.   +11 more
core   +1 more source

Plant Triose Phosphate Isomerase Isozymes [PDF]

Plant Physiology, 1984
We report the first complete purifications of the cytosolic and plastid isozymes of triose phosphate isomerase (TPI; EC 5.3.1.1) from higher plants including spinach (Spinacia oleracea), lettuce (Lactuca sativa), and celery (Apium graveolens). Both isozymes are composed of two isosubunits with approximate molecular weight of 27,000; in spinach and ...
E, Pichersky, L D, Gottlieb
openaire   +2 more sources