Results 31 to 40 of about 8,038 (247)

Studies on the subunit structure and amino acid sequence of triose phosphate isomerase from chicken breast muscle [PDF]

Biochemical Journal, 1974
1. Triose phosphate isomerase was prepared by chromatography on DEAE-cellulose of an (NH4)2SO4 fraction of an extract of homogenized chicken breast muscle. The product is homogeneous on gel electrophoresis and is suitable for growing crystals for X-ray work. The specific activity is 10000 units/mg and the value for E0.1%280 is 1.20. 2.
A J, Furth, J D, Milman, J D, Priddle, R E, Offord   +3 more
openaire   +2 more sources

Post-duplication charge evolution of phosphoglucose isomerases in teleost fishes through weak selection on many amino acid sites-3

, 2011
Copyright information:Taken from "Post-duplication charge evolution of phosphoglucose isomerases in teleost fishes through weak selection on many amino acid sites"http://www.biomedcentral.com/1471-2148/7/204BMC Evolutionary Biology 2007;7():204-204 ...
Mutsumi Nishida (38197), Yukuto Sato (68176)   +1 more
core   +1 more source

Proteomic identification of Drosophila melanogaster male accessory gland proteins, including a pro-cathepsin and a soluble gamma-glutamyl transpeptidase. [PDF]

, 2006
Background In Drosophila melanogaster, the male seminal fluid contains proteins that are important for reproductive success. Many of these proteins are synthesised by the male accessory glands and are secreted into the accessory gland lumen, where they ...
Shirras Alan D, Audsley, Neil, Rylett, Caroline M., Shirras, Alan D., Isaac R Elwyn, Keen Jeff N, Isaac, R. Elwyn, Walker, Michael J., Sajid Mohammed, Sajid, Mohammed, Audsley Neil, Walker Michael J, Keen, Jeffrey N., Rylett Caroline M   +13 more
core   +1 more source

Functions of the conserved anionic amino acids and those interacting with the substrate phosphate group of phosphoglucose isomerase

FEBS Letters, 2001
Phosphoglucose isomerase catalyzes the isomerization between glucose 6‐phosphate and fructose 6‐phosphate in cytoplasm, and functions as autocrine motility factor and neuroleukin outside the cells. A phosphoglucose isomerase from Bacillus stearothermophilus (pgiA) was subjected to mutagenesis study to address the catalytic function of the conserved ...
Meng, Menghsiao, Lin, Hua-Yang, Hsieh, Chia-Jung, Chen, Yen-Ting   +3 more
openaire   +2 more sources

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Identification of essential amino acid residues in a sterol 8,7-isomerase from Zea mays reveals functional homology and diversity with the isomerases of animal and fungal origin [PDF]

Biochemical Journal, 2008
A putative 8,7SI (sterol 8,7-isomerase) from Zea mays, termed Zm8,7SI, has been isolated from an EST (expressed sequence tag) library and subcloned into the yeast erg2 mutant lacking 8,7SI activity. Zm8,7SI restored endogenous ergosterol synthesis. An in vitro enzymatic assay in the corresponding yeast microsomal extract indicated that the preferred Δ8-
Rahier, A., Pierré, S., Riveill, G., Karst, F.   +3 more
openaire   +3 more sources

Malectin Alleviates Endoplasmic Reticulum Stress in Gestational Diabetes Mellitus via Glycoprotein Quality Control Mechanisms

Advanced Science, EarlyView.
Malectin alleviates high glucose‐induced ER stress and damage in placental trophoblasts, a function dependent on its six critical carbohydrate‐binding residues. In a GDM mouse model, administration of TAT‐Malectin ameliorated hyperglycemia and placental ER stress and prevented fetal macrosomia.
Jiahui Zhu, Yumeng Zhang, Ye Wang, Xiaoyu Zhu, Ailin Yuan, Weijing Yin, Huangmin Yu, Xuemin Pang, Yufeng He, Yuchen Wang, Tong Wang, Yong Li, Yunlong Si   +12 more
wiley   +1 more source

Mapping the stereospecificity of peptidyl prolyl cis/trans isomerases

, 1998
The stereospecificity of peptidyl prolyl cis/trans isomerases (PPIases) was studied using tetrapeptide substrate analogs in which one amino acid residue was replaced by the cognate d-amino acid in various positions of the peptide chain.
Reimer, Ulf, Schutkowski, Mike, Mike Schutkowski, Gunter Fischer, Ulf Reimer, Schiene, Cordelia, Cordelia Schiene, Fischer, Gunter   +7 more
core   +1 more source

The Eps1p Protein Disulfide Isomerase Conserves Classic Thioredoxin Superfamily Amino Acid Motifs but Not Their Functional Geometries

PLoS ONE, 2014
The widespread thioredoxin superfamily enzymes typically share the following features: a characteristic α-β fold, the presence of a Cys-X-X-Cys (or Cys-X-X-Ser) redox-active motif, and a proline in the cis configuration abutting the redox-active site in the tertiary structure. The Cys-X-X-Cys motif is at the solvent-exposed amino terminus of an α-helix,
Shai Biran, Yair Gat, Deborah Fass
openaire   +5 more sources