Results 21 to 30 of about 13,885 (249)

Characterization of In Planta—Induced Rust Genes Isolated from a Haustorium-Specific cDNA Library

open access: yesMolecular Plant-Microbe Interactions, 1997
Rust fungi are plant parasites that depend on living host tissue for growth. For invasion of leaves, dikaryotic urediospores differentiate germ tubes and infection structures that penetrate through stomata.
Matthias Hahn, Kurt Mendgen
doaj   +1 more source

Bivalent cations and amino‐acid composition contribute to the thermostability of Bacillus licheniformis xylose isomerase [PDF]

open access: yesEuropean Journal of Biochemistry, 2001
Comparative analysis of genome sequence data from mesophilic and hyperthermophilic micro‐organisms has revealed a strong bias against specific thermolabile amino‐acid residues (i.e. N and Q) in hyperthermophilic proteins. The N + Q content of class II xylose isomerases (XIs) from mesophiles, moderate thermophiles, and hyperthermophiles was examined. It
C, Vieille   +3 more
openaire   +2 more sources

Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene [PDF]

open access: yes, 2005
Sucrose isomerase (SI) genes from Pantoea dispersa UQ68J, Klebsiella planticola UQ14S, and Erwinia rhapontici WAC2928 were cloned and expressed in Escherichia coli. The predicted products of the UQ14S and WAC2928 genes were similar to known SIs.
Wu, L. Q., Birch, R. G.
core   +1 more source

Importance of disulphide bonds for vaccinia virus L1R protein function

open access: yesVirology Journal, 2005
L1R, a myristylated late gene product of vaccinia virus, is essential for formation of infectious intracellular mature virions (IMV). In its absence, only viral particles arrested at an immature stage are detected and no infectious progeny virus is ...
Hruby Dennis E   +2 more
doaj   +1 more source

A theoretical estimate for nucleotide sugar demand towards Chinese Hamster Ovary cellular glycosylation [PDF]

open access: yes, 2016
Glycosylation greatly influences the safety and efficacy of many of the highest-selling recombinant therapeutic proteins (rTPs). In order to define optimal cell culture feeding strategies that control rTP glycosylation, it is necessary to know how ...
Jimenez del Val, I   +2 more
core   +1 more source

Study on Differential Protein Expression in Natural Selenium-Enriched and Non-Selenium-Enriched Rice Based on iTRAQ Quantitative Proteomics

open access: yesBiomolecules, 2019
This work was designated to scrutinize the protein differential expression in natural selenium-enriched and non-selenium-enriched rice using the Isobaric-tags for relative and absolute quantification (iTRAQ) proteomics approach.
Rui Zeng   +7 more
doaj   +1 more source

A Critical Test of the Electrostatic Contribution to Catalysis with Noncanonical Amino Acids in Ketosteroid Isomerase [PDF]

open access: yesJournal of the American Chemical Society, 2016
The vibrational Stark effect (VSE) has been used to measure the electric field in the active site of ketosteroid isomerase (KSI). These measured fields correlate with ΔG(⧧) in a series of conventional mutants, yielding an estimate for the electrostatic contribution to catalysis (Fried et al. Science 2014, 346, 1510-1513).
Yufan, Wu, Steven G, Boxer
openaire   +2 more sources

Structural Insights into the Epimerization of β-1,4-Linked Oligosaccharides Catalyzed by Cellobiose 2-Epimerase, the Sole Enzyme Epimerizing Non-anomeric Hydroxyl Groups of Unmodified Sugars [PDF]

open access: yes, 2013
Cellobiose 2-epimerase (CE) reversibly converts d-glucose residues into d-mannose residues at the reducing end of unmodified β1,4-linked oligosaccharides, including β-1,4-mannobiose, cellobiose, and lactose.
Fujiwara, Takaaki   +4 more
core   +1 more source

Introduction to protein folding for physicists [PDF]

open access: yes, 2007
The prediction of the three-dimensional native structure of proteins from the knowledge of their amino acid sequence, known as the protein folding problem, is one of the most important yet unsolved issues of modern science.
Abagyan R. A.   +21 more
core   +3 more sources

Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

open access: yesBMC Biotechnology, 2017
Background Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel ...
Beatriz Temer   +9 more
doaj   +1 more source

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