Results 101 to 110 of about 238,366 (195)

Preparation of Fungal ITS Amplicons from Soil Samples for Illumina Amplicon Sequencing v1

, 2018
This protocol was used for the molecular profiling of the soil fungal community of two winter wheat fields as a part of a long-term field trial in Germany with following Illumina Amplicon Sequencing. Besides different pre-crops, the effects of soil management and fertilization on the fungal communities were investigated. Due to different aspects of our
Loreen Sommermann, Joerg Geistlinger, Jessica Zwanzig, Ingo Schellenberg   +3 more
openaire   +1 more source

Amplicon sequencing on the MinION platform v3

, 2021
Amplicon sequencing on the MinION ...
openaire   +1 more source

Amplicon sequencing on the MinION platform v2

, 2021
Amplicon sequencing on the MinION ...
openaire   +1 more source

UCHIME2: improved chimera prediction for amplicon sequencing [PDF]

, 2016
Amplicon sequencing generates chimeric reads which can cause spurious inferences of biological variation. I describe UCHIME2, an update of the popular UCHIME chimera detection algorithm with new modes optimized for high-resolution biological sequence reconstruction (“denoising”) and other applications. I show that chimera frequency correlates inversely
openaire   +1 more source

Complete pipeline for Oxford Nanopore Technology amplicon sequencing (ONT‐AmpSeq): from pre‐processing to creating an operational taxonomic unit table

FEBS Open Bio
Amplicon sequencing has long served as a robust method for characterising microbial communities, and despite inherent resolution limitations, it remains a preferred technique, offering cost‐ and time‐effective insights into bacterial compositions.
Patrick Skov Schacksen, Stine Karstenskov Østergaard, Mathias Helmer Eskildsen, Jeppe Lund Nielsen   +3 more
doaj   +1 more source

Amplicon Sequencing for Genotyping S. Typhi v1

The following protocol is for amplifying and sequencing amplicons targeting Salmonella Typhi. It is primarily for use with samples that are already suspected to be positive for S. Typhi and has been designed for use with DNA extracted from environmental surveillance samples.
Jaspreet Mahindroo, Catherine Troman, Nick Grassly   +2 more
openaire   +1 more source

Nanopore amplicon sequencing withR10.4.1 flow cells v1

This protocol describes an updated method for Nanopore amplicon sequencing withR10.4.1 flow cells, building upon the original protocol "Nanopore amplicon sequencing" by Yoshiyuki Matsuo (DOI: https://dx.doi.org/10.17504/protocols.io.8epv5zrodv1b/v4).
Teng Li, Miao Wang, Zhengzheng Zhu, Allen Rodrigo   +3 more
openaire   +1 more source

An accurate and efficient method for large-scale SSR genotyping and applications [PDF]

, 2017
Chen, Hong, Chen, Lihong, Fang, Zhiwei, Gao, Lifen, Hu, Zhangfeng, Li, Lun, Lu, Long, Ma, Hongyu, Peng, Hai, Ren, Sheng, Zhang, Weiziong, Zhou, Junfei   +11 more
core   +2 more sources

Amplicon Sequencing for Genotyping S. Typhi v2

The following protocol is for amplifying and sequencing amplicons targeting Salmonella Typhi. It is primarily for use with samples that are already suspected to be positive for S. Typhi and has been designed for use with DNA extracted from environmental surveillance samples.
Jaspreet Mahindroo, Anton Spadar, Catherine Troman, Zoe Dyson, Kathryn Holt, Nick Grassly   +5 more
openaire   +1 more source

Can Amplicon Sequencing Be Replaced by Metagenomics for Biodiversity Inventories? [PDF]

Mol Ecol Resour
Elliott L, Coissac E.
europepmc   +1 more source