Results 101 to 110 of about 237,694 (293)

TOAST: a novel tool for designing targeted gene amplicons and an optimised set of primers for high-throughput sequencing in tuberculosis genomic studies

BMC Genomics
Background Amplicon sequencing of Mycobacterium tuberculosis resistance-associated genes offers a cost-effective alternative to whole-genome sequencing for rapid profiling of infections and guiding clinical management.
Linfeng Wang, Naphatcha Thawong, Joseph Thorpe, Matthew Higgins, Mark Tan Kia Ik, Waritta Sawaengdee, Surakameth Mahasirimongkol, João Perdigão, Susana Campino, Taane G. Clark, Jody E. Phelan   +10 more
doaj   +1 more source

A short plus long-amplicon based sequencing approach improves genomic coverage and variant detection in the SARS-CoV-2 genome.

PLoS ONE, 2022
High viral transmission in the COVID-19 pandemic has enabled SARS-CoV-2 to acquire new mutations that may impact genome sequencing methods. The ARTIC.v3 primer pool that amplifies short amplicons in a multiplex-PCR reaction is one of the most widely used
Carlos Arana, Chaoying Liang, Matthew Brock, Bo Zhang, Jinchun Zhou, Li Chen, Brandi Cantarel, Jeffrey SoRelle, Lora V Hooper, Prithvi Raj   +9 more
doaj   +1 more source

Gut Microbiota‐Derived Anandamide Mediates the Therapeutic Effects of Urolithin A on Alcohol‐Induced Cognitive and Social Dysfunction via CB1R‐DRD2‐RAP1 Signaling Axis

Advanced Science, EarlyView.
This study reveals that Urolithin A (UA) counteracts alcohol‐induced cognitive and social dysfunction (AICSD) via a gut microbiome‐dependent mechanism. UA‐enriched Bacteroids sartorii and Parabacteroids distasonis elevate anandamide (AEA), which activates the CB1R‐DRD2‐Rap1 signaling cascade to drive synaptic repair and reduce neuroinflammation ...
Hongbo Zhang, Zibin Li, Yao Xiao, Ji Bian, Caian He, Chao Liu, Lan Gong, Lin Han, Zhigang Liu, Min Wang   +9 more
wiley   +1 more source

Rapid detection of copy number variations and point mutations in BRCA1/2 genes using a single workflow by ion semiconductor sequencing pipeline [PDF]

, 2018
Molecular analysis of BRCA1 (MIM# 604370) and BRCA2 (MIM #600185) genes is essential for familial breast and ovarian cancer prevention and treatment.
Amanti, C, De Marchis, L, Germani, A, Libi, F, Lombardi, A, Maggi, S, Mattei, M, Pellegrini, P, Piane, M, Pizzuti, A, Stanzani., G, Torrisi, Mr   +11 more
core   +1 more source

Regulating Tumor Metabolic Reprogramming with Biomimetic Co‐Delivery of Simvastatin and Kynureninase for Immunotherapy

Advanced Science, EarlyView.
After the intravenous injection of biomimetic and pH/ROS‐responsive PTSK@CRM, the nanoparticles can be accumulated in tumors and release Sim and KYNase to inhibit the tumor growth, regulate the metabolism of cholesterol and Kyn, and reverse the immunosuppressive tumor microenvironment.
Jiaxin Yin, Shengcai Yang, Zengguang Liu, Songchen Zhao, Siyu Sun, Ziling Liu, Quanshun Li   +6 more
wiley   +1 more source

Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre. [PDF]

, 2019
Sinking particles transport carbon and nutrients from the surface ocean into the deep sea and are considered hot spots for bacterial diversity and activity.
Collins, James R, Farnelid, Hanna, Ossolinski, Justin E, Ploug, Helle, Turk-Kubo, Kendra, Van Mooy, Benjamin AS, Zehr, Jonathan P   +6 more
core   +1 more source

Unveiling Global Diversity of Patescibacteriota and Functional Interactions with Host Microbes

Advanced Science, EarlyView.
Patescibacteriota represents a diverse group of ultra‐small epibiotic bacteria, which is largely overlooked. By integrating ribosomal protein S3‐based community profiling with MAG‐based metabolic potential analyses, this study provides new insights into their distribution, diversity, and potential interactions with other bacteria across diverse ...
Yanhan Ji, Xu Liu, Shuai Zhao, Sihan Zhou, Yufan Yang, Ping Zhang, Yu Shi, Wei Qin, Guibing Zhu, Yongguan Zhu, Yanzheng Gao, Jiandong Jiang, Baozhan Wang   +12 more
wiley   +1 more source

Triplicate PCR reactions for 16S rRNA gene amplicon sequencing are unnecessary

BioTechniques, 2019
Conventional wisdom holds that PCR amplification for sequencing should employ pooled replicate reactions to reduce bias due to jackpot effects and chimera formation.
Clarisse Marotz, Anukriti Sharma, Greg Humphrey, Neil Gottel, Christopher Daum, Jack A Gilbert, Emiley Eloe-Fadrosh, Rob Knight   +7 more
doaj   +1 more source

ThermoPhyl : a software tool for selecting phylogenetically optimized conventional and quantitative-PCR taxon-targeted assays for use with complex samples [PDF]

, 2011
The ability to specifically and sensitively target genotypes of interest is critical for the success of many PCR-based analyses of environmental or clinical samples that contain multiple templates.Next-generation sequence data clearly show that such ...
Dowd, Scot E., Oakley, Brian B., Purdy, Kevin J.   +2 more
core   +1 more source

Western Diet Inhibits FUT7‐Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease

Advanced Science, EarlyView.
In this study, we constructed a nanoparticle that was CD4 antibody‐mediated targeted, Fut7‐expressing plasmid‐loaded cationic liposome, namely CD4‐LDP‐Fut7. Upregulation of Fut7 expression in Tregs by using CD4‐LDP‐Fut7 can increase Treg homing to the intestine, thereby facilitating repair of the intestinal epithelial barrier and inhibiting ...
Qian Zhou, Xianfei Wang, Teng Ben, Chunping Zhu, Xinlong Lin, Yinmu Li, Xinyue Zhang, Yeling Chen, Yuchen Xuan, Huaiwen Chen, Ke Liu, Fang Wu, Yuexin Ren, Gao Tan   +13 more
wiley   +1 more source