Results 51 to 60 of about 238,366 (195)
Forensic SNP genotyping using nanopore MinION sequencing [PDF]
, 2017 One of the latest developments in next generation sequencing is the Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied the applicability of this system to perform forensic genotyping of the forensic female DNA standard 9947 A using
Cornelis, Senne +4 more
core +2 more sources
Differential Functional Constraints Cause Strain-Level Endemism in Polynucleobacter Populations. [PDF]
, 2016 The adaptation of bacterial lineages to local environmental conditions creates the potential for broader genotypic diversity within a species, which can enable a species to dominate across ecological gradients because of niche flexibility.
Geeta Rijal +8 more
core +2 more sources
Identification of a novel ß-adrenergic octopamine receptor-like gene (ßAOR-like) and increased ATP-binding cassette B10 (ABCB10) expression in a Rhipicephalus microplus cell line derived from acaricide-resistant ticks [PDF]
, 2016 Background: The cattle tick Rhipicephalus (Boophilus) microplus is an economically important parasite of livestock. Effective control of ticks using acaricides is threatened by the emergence of resistance to many existing compounds. Several continuous R.
Bell-Sakyi, Lesley +4 more
core +1 more source
Novel Amplicon-Based Sequencing Approach to West Nile Virus
Viruses, 2023 West Nile virus is a re-emerging arbovirus whose impact on public health is increasingly important as more and more epidemics and epizootics occur, particularly in America and Europe, with evidence of active circulation in Africa. Because birds constitute the main reservoirs, migratory movements allow the diffusion of various lineages in the world.
Moussa Moïse Diagne +28 more
openaire +4 more sources
Frontiers in Microbiology, 2022 The Illumina MiSeq platform has been widely used as a standard method for studying the rumen microbiota. However, the low resolution of taxonomic identification is the only disadvantage of MiSeq amplicon sequencing, as it targets a part of the 16S rRNA ...
Hiroto Miura +12 more
doaj +1 more source
Drug resistance outcomes of long-term ART with tenofovir disoproxil fumarate in the absence of virological monitoring [PDF]
, 2018 Objectives: The resistance profiles of patients receiving long-term ART in sub-Saharan Africa have been poorly described. This study obtained a sensitive assessment of the resistance patterns associated with long-term tenofovir-based ART in a ...
Appiah, Lambert T +9 more
core +3 more sources
iScience, 2020 Summary: Synthetic bacterial communities are powerful tools for studying microbial ecology and evolution, as they enable rapid iteration between controlled laboratory experiments and theoretical modeling. However, their utility is hampered by the lack of
Nathan Cermak +2 more
doaj +1 more source
Choice of DNA extraction method affects detection of bacterial taxa from retail chicken breast
BMC Microbiology, 2022 Background Sequence-based methods for the detection of bacteria such as 16S rRNA amplicon sequencing and metagenomics can provide a comprehensive view of the bacterial microbiome of food.
Annika Flint +8 more
doaj +1 more source
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons [PDF]
Journal of Visualized Experiments, 2014 One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA.
Sanschagrin, Sylvie, Yergeau, Étienne
openaire +2 more sources
Scientific Reports, 2017 Modern metagenomic environmental DNA studies are almost completely reliant on next-generation sequencing, making evaluations of these methods critical. We compare two next-generation sequencing techniques – amplicon and shotgun – on water samples across ...
Michael Tessler +11 more
doaj +1 more source