454 screening of individual MHC variation in an endemic island passerine [PDF]
, 2014 Genes of the major histocompatibility complex (MHC) code for receptors that are central to the adaptive immune response of vertebrates. These genes are therefore important genetic markers with which to study adaptive genetic variation in the wild.
Gonzalez Quevedo, Catalina +3 more
core +2 more sources
Evidence of global-scale aeolian dispersal and endemism in isolated geothermal microbial communities of Antarctica [PDF]
, 2014 New evidence in aerobiology challenges the assumption that geographical isolation is an effective barrier to microbial transport. However, given the uncertainty with which aerobiological organisms are recruited into existing communities, the ultimate ...
Cary, S. Craig +3 more
core +2 more sources
Amplicon sequencing of colorectal cancer: variant calling in frozen and formalin-fixed samples. [PDF]
PLoS ONE, 2015 Next generation sequencing (NGS) is an emerging technology becoming relevant for genotyping of clinical samples. Here, we assessed the stability of amplicon sequencing from formalin-fixed paraffin-embedded (FFPE) and paired frozen samples from colorectal
Johannes Betge +11 more
doaj +1 more source
PLoS ONE, 2023 The global distribution and constant evolution are challenges for the control of porcine reproductive and respiratory syndrome virus (PRRSV), one of the most important viruses affecting swine worldwide. Effective control of PRRSV benefits from genotyping,
Leonardo Cardia Caserta +3 more
doaj +1 more source
Diverse diazotrophs are present on sinking particles in the North Pacific Subtropical Gyre. [PDF]
, 2019 Sinking particles transport carbon and nutrients from the surface ocean into the deep sea and are considered hot spots for bacterial diversity and activity.
Collins, James R +6 more
core +1 more source
mSphere, 2018 DNA sequencing and analysis methods were compared for 16S rRNA V4 PCR amplicon and genomic DNA (gDNA) mock communities encompassing nine bacterial species commonly found in milk and dairy products.
Zhengyao Xue +2 more
doaj +1 more source
Application of high-throughput sequencing to whole rabies viral genome characterisation and its use for phylogenetic re-evaluation of a raccoon strain incursion into the province of Ontario [PDF]
, 2017 Raccoon rabies remains a serious public health problem throughout much of the eastern seaboard of North America due to the urban nature of the reservoir host and the many challenges inherent in multi-jurisdictional efforts to administer co-ordinated and ...
Biek, Roman +4 more
core +1 more source
BMC GenomicsBackground Amplicon sequencing of Mycobacterium tuberculosis resistance-associated genes offers a cost-effective alternative to whole-genome sequencing for rapid profiling of infections and guiding clinical management.
Linfeng Wang +10 more
doaj +1 more source
Decoding fungal communities: ITS extraction matters more than ASVs vs. OTUs [PDF]
Web EcologyTo capture the complexity of fungal communities, microbial ecologists must often resort to amplicon sequencing. Amplicon sequences are obtained from the nuclear ribosomal internal transcribed spacer (ITS), which is currently used as a marker for fungal ...
G. Tosadori, J. Bosch
doaj +1 more source
BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
, 2016 Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
core +2 more sources