The current study employed both amplicon and shotgun sequencing to examine and compare the rumen microbiome in Angus bulls fed with either a backgrounding diet (BCK) or finishing diet (HG), to assess if both methods produce comparable results.
Mi Zhou +7 more
doaj +1 more source
DNA methylation profiling of the human major histocompatibility complex: A pilot study for the Human Epigenome Project [PDF]
The Human Epigenome Project aims to identify, catalogue, and interpret genome-wide DNA methylation phenomena. Occurring naturally on cytosine bases at cytosine-guanine dinucleotides, DNA methylation is intimately involved in diverse biological processes ...
Andrews, TD +13 more
core
BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien +7 more
core +2 more sources
Sample Preparation for Amplicon Sequencing v1
Preparation of PCR products for amplicon sequencing
Anna Székely, Máté Vass
openaire +1 more source
Proteins, Processing, and Properties of Adhesive Fluid Condensates Purified from Mussels
Mussels exhibit an unmatched proficiency for adhering to wet surfaces in salty environments—a remarkable ability that could inspire new biomedical and technical glues. The fluid protein condensates used to form the underwater mussel glue are extracted, reconstituted and characterized with advanced spectroscopy and nanomechanical analysis, revealing ...
Mathieu D. Rivard +8 more
wiley +1 more source
454 screening of individual MHC variation in an endemic island passerine [PDF]
Genes of the major histocompatibility complex (MHC) code for receptors that are central to the adaptive immune response of vertebrates. These genes are therefore important genetic markers with which to study adaptive genetic variation in the wild.
Gonzalez Quevedo, Catalina +3 more
core +2 more sources
A methacrylamide β‐cyclodextrin‐based nanogel (MACD nGel) is developed to load the antimicrobial drug Metronidazole (MNZ) for topical delivery for the treatment of periodontitis. It is demonstrated that cyclodextrin nanogel (nGel) loaded with metronidazole provides an efficient drug delivery route but also has potential clinical applications and offers
Yanjing Ji +8 more
wiley +1 more source
The global distribution and constant evolution are challenges for the control of porcine reproductive and respiratory syndrome virus (PRRSV), one of the most important viruses affecting swine worldwide. Effective control of PRRSV benefits from genotyping,
Leonardo Cardia Caserta +3 more
doaj +1 more source
Use of Whole Genome Phylogeny and Comparisons in the Development of a Multiplex-PCR Assay to Identify Sequence Type 36 Vibrio parahaemolyticus [PDF]
Vibrio parahaemolyticus sequence type (ST) 36 strains that are native to the Pacific Ocean have recently caused multi-state outbreaks of gastroenteritis linked to shellfish harvested from the Atlantic Ocean.
Cooper, Vaughn S. +6 more
core +1 more source
Application of high-throughput sequencing to whole rabies viral genome characterisation and its use for phylogenetic re-evaluation of a raccoon strain incursion into the province of Ontario [PDF]
Raccoon rabies remains a serious public health problem throughout much of the eastern seaboard of North America due to the urban nature of the reservoir host and the many challenges inherent in multi-jurisdictional efforts to administer co-ordinated and ...
Biek, Roman +4 more
core +1 more source

