Results 121 to 130 of about 13,480 (279)

Direct identification of amyloids by label-free quantitative LC-MS

, 2016
Direct identification of amyloids by label-free quantitative LC-MSH. N. Danielsen, S. H. Hansen, F.-A. Herbst, P. H. Nielsen, M. S. DueholmAmyloids are highly ordered fibrillar protein polymers used by organisms from all domains of life due to their ...
Hansen, Susan Hove, Danielsen, Heidi Nolsøe, Herbst, Florian-Alexander, Dueholm, Morten Simonsen; id_orcid, Nielsen, Per Halkjær   +4 more
core  

Deconstruction of Human Age‐Related Cataract Capsules Defines Aging

Advanced Science, EarlyView.
We generate a comprehensive atlas of age‐related cataracts (ARC) at a single‐cell resolution, encompassing three disease states—mild cataract group (Mild), severe cortical cataract group (Severe_C), and severe nuclear cataract group (Severe_N). We find that ARC involves seven distinct lens capsule cell types, with notable differences in cellular ...
Qiaomei Tang, Ziyang Tong, Chunmei Fan, Silong Chen, Jiarui Guo, Jianghua Hu, Ke Yao, Zi Yin, Xiao Chen, Yibo Yu   +9 more
wiley   +1 more source

Functional Amyloids Used by Organisms::A Lesson in Controlling Assembly

, 2010
Amyloid has been studied primarily in relation to the disease states that it causes in humans, but in recent years, a range of proteins have been identified that self-assemble to a β-sheet rich fold as part of their native function and as such have been ...
SCHEIBEL, T, Andrew M. Smith, Smith, Andrew, Thomas Scheibel   +3 more
core   +1 more source

Neuron‐Targeted Exosomal Delivery of siRNA Against RIPK3 Slows Neurodegenerative Progression in Alzheimer's Disease

Advanced Science, EarlyView.
Engineered exosomal siRNA delivery platform enables systemic, neuron‐targeted RNA transport across the blood–brain barrier. Surface functionalization with rabies virus glycoprotein‐derived peptide facilitates receptor‐mediated transcytosis, achieving efficient cytosolic delivery and robust gene silencing. Targeting RIPK3 suppresses necroptosis, reduces
Chi Zhang, Jiaqi Zhang, Yuzhi Wang, Yaodong Wang, Peng Lin, Yanling Wang, Zixuan Tang, Jintai Yu, Qin Zhou, Feiyun Cui   +9 more
wiley   +1 more source

Astrocytes in Amyloid Generation and Alcohol Metabolism: Implications of Alcohol Use in Neurological Disorder(s)

Cells
As per the National Survey on Drug Use and Health, 10.5% of Americans aged 12 years and older are suffering from alcohol use disorder, with a wide range of neurological disorders.
Mohit Kumar, Natalie Swanson, Sudipta Ray, Shilpa Buch, Viswanathan Saraswathi, Susmita Sil   +5 more
doaj   +1 more source

Aggregating gut: on the link between neurodegeneration and bacterial functional amyloids [PDF]

Amyloids are insoluble protein aggregates with a cross-beta structure, which are traditionally associated with neurodegeneration. Similar structures, named functional amyloids, expressed mostly by microorganisms, play important physiological roles, e.g ...
Zielinska, K., Wojciechowski, J., Kosciolek, T., Kotulska, M., Wojciechowska, A., Söding, J. ; https://orcid.org/   +5 more
core   +1 more source

Structural Polymorphism of polyG Inclusions Revealed by In Situ Cryo‐Electron Tomography

Advanced Science, EarlyView.
Correlative cryo‐electron tomography in primary cortical neurons and NIID mouse brain tissue reveals that polyG inclusions are interconnected ribbon‐like assemblies rather than canonical amyloid fibrils. Multiple compartment‐specific ribbon states show distinct 26S proteasome accessibility, while cytoplasmic ribbons contact and deform ER‐like ...
Yunwen Qian, Yalan Wan, Chen Chen, Xiaoyu Zheng, Wenjing Du, Junhan Yang, Zhihao Quan, Biyu Yang, Jiaxi Yu, Jie Zheng, Zhaoxia Wang, Jianwen Deng, Qiang Guo   +12 more
wiley   +1 more source

Anionic Lipid Catalyzes the Generation of Cytotoxic Insulin Oligomers

Biomolecules
The misfolding and aggregation of proteins into amyloidogenic assemblies are key features of several metabolic and neurodegenerative diseases. Human insulin has long been known to form amyloid fibrils under various conditions, which affects its ...
Jhinuk Saha, Audrey Wolszczak, Navneet Kaur, Malitha C. Dickwella Widanage, Samuel D. McCalpin, Riqiang Fu, Jamel Ali, Ayyalusamy Ramamoorthy   +7 more
doaj   +1 more source

A Phosphorylation‐Induced Micellization Switch in the Low‐Complexity Domain of TDP‐43

Advanced Science, EarlyView.
Phosphorylation of TAR DNA‐binding protein's 43 kDa (TDP‐43) low‐complexity domain by casein kinase 1 delta (CK1δ) acts as a molecular switch, redirecting its self‐assembly from macroscopic phase separation toward finite‐sized, spherical block‐copolymer micelles of ∼30 nm.
Rodrigo F. Dillenburg, Anastasia Lopatina, Hao Ruan, Tom Scheidt, Simone Mosna, Emre Pekbilir, Julia Bieber, Frank Schäfer‐Depoix, Katharina Landfester, Carla Schmidt, Martin M. Möckel, Svenja Morsbach, Friederike Schmid, Dorothee Dormann, Lukas Stelzl, Martin Girard, Edward A. Lemke   +16 more
wiley   +1 more source

PolyG Fibrils Coalesce Into Nuclear Ribbons That Engage Proteostasis Machinery in Neuronal Intranuclear Inclusion Disease

Advanced Science, EarlyView.
In NIID, expanded NOTCH2NLC repeats give rise to nuclear polyG inclusions. Tracer‐guided in situ cryo‐electron tomography enables cross‐scale structural analysis from mouse brain to native neuronal nuclei, revealing dense‐core/peripheral‐halo inclusions built from compact polyG ribbons.
Hui Dong, Yongcheng Pan, Zhiyao Tang, Yuxuan Yao, Guicong Zhang, Junpu Wang, Haonan Xiao, Yun Tian, Beisha Tang, Dan Li, Qiang Guo, Ruijun Tian, Qiong Liu, Cong Liu   +13 more
wiley   +1 more source