Results 41 to 50 of about 4,831,623 (248)

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Metabolomics in Preclinical Drug Safety Assessment: Current Status and Future Trends

Metabolites
Metabolomics is emerging as a powerful systems biology approach for improving preclinical drug safety assessment. This review discusses current applications and future trends of metabolomics in toxicology and drug development.
Fenna Sillé, Thomas Hartung
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis

FEBS Letters, EarlyView.
Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee, Junho Jeong, Chae Yeong Lee, Hyunjoon Cho, Man Hwan Oh, Je Chul Lee, Jee‐Young Lee, Yangmee Kim   +7 more
wiley   +1 more source

Associations between clinical signs and pathological findings in toxicity testing

, 2021
Animal testing for toxicity assessment of chemicals and pharmaceuticals must take the 3R principles into consideration. During toxicity testing in vivo, clinical signs are used to monitor animal welfare and to inform about potential toxicity.
Silva, Antero Vieira,, Silva, Antero, V,, Liiv, E, Tornqvist, E, Öberg, Mattias,, Liiv, Elin,, Törnqvist, Elin,, Öberg, Mattias, Platzack, Björn,, Silva, Antero, Platzack, Björn, Norinder, U, Liiv, Elin, Törnqvist, Elin, Silva, AV, Platzack, B, Oberg, M, Norinder, Ulf,   +17 more
core   +1 more source

Guidance on assessing the methodological and reporting quality of toxicologically relevant studies: A scoping review

Environment International, 2016
Assessments of methodological and reporting quality are critical to adequately judging the credibility of a study's conclusions and to gauging its potential reproducibility.
Gbeminiyi O. Samuel, Sebastian Hoffmann, Robert A. Wright, Manoj Mathew Lalu, Grace Patlewicz, Richard A. Becker, George L. DeGeorge, Dean Fergusson, Thomas Hartung, R. Jeffrey Lewis, Martin L. Stephens   +10 more
doaj   +1 more source

Effect of sub-chronic exposure to cigarette smoke, electronic cigarette and waterpipe on human lung epithelial barrier function

BMC Pulmonary Medicine, 2020
Background Taking into consideration a recent surge of a lung injury condition associated with electronic cigarette use, we devised an in vitro model of sub-chronic exposure of human bronchial epithelial cells (HBECs) in air-liquid interface, to ...
Baishakhi Ghosh, Hermes Reyes-Caballero, Sevcan Gül Akgün-Ölmez, Kristine Nishida, Lakshmana Chandrala, Lena Smirnova, Shyam Biswal, Venkataramana K. Sidhaye   +7 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Green toxicology only becomes beautiful through AI

Frontiers in Chemistry
Green Toxicology extends the principles of Green Chemistry by embedding toxicological foresight into chemical design, with the aim of preventing hazards before substances reach markets or the environment.
Alexandra Maertens, Thomas Hartung, Thomas Hartung, Thomas Hartung   +3 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source