Results 61 to 70 of about 284,210 (304)

Multiparametric Material Functionality of Microtissue‐Based In Vitro Models as Alternatives to Animal Testing

Advanced Science, 2022
With the definition of the 3R principle by Russel and Burch in 1959, the search for an adequate substitute for animal testing has become one of the most important tasks and challenges of this time, not only from an ethical, but also from a scientific ...
Elena Stengelin, Julian Thiele, Sebastian Seiffert   +2 more
doaj   +1 more source

A Scientific Roadmap for Antibiotic Discovery: A Sustained and Robust Pipeline of New Antibacterial Drugs and Therapies is Critical to Preserve Public Health [PDF]

, 2016
In recent decades, the discovery and development of new antibiotics have slowed dramatically as scientific barriers to drug discovery, regulatory challenges, and diminishing returns on investment have led major drug companies to scale back or abandon ...
Allan Coukell, Carolyn Shore, Elizabeth Jungman, Kathy Talkington, Pooja Kothari, Susan K. Urahn   +5 more
core  

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

In vitro and in silico Models to Study Mosquito-Borne Flavivirus Neuropathogenesis, Prevention, and Treatment

Frontiers in Cellular and Infection Microbiology, 2019
Mosquito-borne flaviviruses can cause disease in the nervous system, resulting in a significant burden of morbidity and mortality. Disease models are necessary to understand neuropathogenesis and identify potential therapeutics and vaccines.
Megan Chesnut, Laura S. Muñoz, Laura S. Muñoz, Georgina Harris, Dana Freeman, Lucio Gama, Lucio Gama, Carlos A. Pardo, Carlos A. Pardo, David Pamies, David Pamies   +10 more
doaj   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

AI snake oil? A risk/benefit analysis for toxicology

Frontiers in Artificial Intelligence
Artificial intelligence (AI) is increasingly used to support predictive, mechanistic, and human-relevant toxicology at scale. However, its integration into regulatory science - particularly in drug development - remains uneven, because encouraging ...
Thomas Hartung, Thomas Hartung, Thomas Hartung, Mohan Rao, Mamta Behl, Alexandra Maertens   +5 more
doaj   +1 more source

Optimal Representation of Anuran Call Spectrum in Environmental Monitoring Systems Using Wireless Sensor Networks [PDF]

, 2018
The analysis and classification of the sounds produced by certain animal species, notably anurans, have revealed these amphibians to be a potentially strong indicator of temperature fluctuations and therefore of the existence of climate change ...
Aguayo-González, Francisco (Coordinador), Barbancho Concejero, Julio, Carrasco Muñoz, Alejandro, Gómez-Bellido, Jesús, León de Mora, Carlos (Coordinador), Luque Sendra, Amalia   +5 more
core  

Giving meaning to alternative methods to animal testing

ALTEX, 2018
The 3 rd edition of the advanced theoretical-training course “ Giving meaning to alternative methods to animal testing ” was held in Genoa on July 6-7, 2017. The theoretical modules included talks by specialists from companies engaged in the field of advanced in vitro technologies, who offered participants the possibility to try out their technologies ...
Scanarotti C, Rovida C, Penco S, Vernqazza S, Tirendi S, Baldelli I, Ciliberti R, Bassi AM   +7 more
openaire   +3 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source