Results 81 to 90 of about 57,035 (299)

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Animal testing and its alternatives : the most important omics is economics [PDF]

, 2018
For a long time, the discussion about animal testing vs its alternatives centered on animal welfare. This was a static warfare, or at least a gridlock, where life scientists had to take a position and make their value choices and hardly anyone changed ...
Meigs, Lucy, Leist, Marcel, Hartung, Thomas, Smirnova, Lena, Rovida, Costanza   +4 more
core   +1 more source

Advice for bad computational toxicologists

NAM Journal
We recently gave advice for bad (traditional) toxicologists (Rabbit, 2025)一but we also have advice for the new breed of computational toxicologists. So many ways to do a lousy job…
Srijit Seal, Richard R. Rabbit (aka Thomas Hartung)   +1 more
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Confronting the bias towards animal experimentation (animal methods bias)

Frontiers in Drug Discovery
Laws and policies are in place around the world to promote the replacement and reduction of nonhuman animals in science. These principles are rooted not just in ethical considerations for animals, but also in scientific considerations regarding the ...
Catharine E. Krebs, Kathrin Herrmann, Kathrin Herrmann   +2 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The european consensus platform for 3R alternatives to animal experimentation (ecopa) [PDF]

, 2018
International audienceThe ecopa is a non-profit organization which explores all possible means to improve the exchange of information in the area of alternative methods.
Bastos, L., Bastos, Henri, Hubert, Philippe, Rovida, Costanza, Heinonen, T.   +4 more
core  

ATERA: Advanced 3D human tissue models and exclusive testing services for cosmetic, consumer product and pharmaceutical industry [PDF]

, 2022
S.44In line with industrial innovation and many EU regulatory encouragements or requirements (EC 1223/2009, 2010/63/EU, EC 1272/2008, EC 1907/2006, 98/8/EC), the need for reconstructed human tissue models is still increasing.
Wever, Bart de, Walles, Heike, Groeber-Becker, Florian, Varlet, Beatrice le, Guillon, Fabien, Ginosian, Anna   +5 more
core  

AI redefines mass spectrometry chemicals identification: retention time prediction in metabolomics and for a Human Exposome Project

Frontiers in Public Health
The comprehensive identification of environmental and endogenous chemicals in human biospecimens is a critical bottleneck for realizing the Human Exposome Project.
Fenna C. M. Sillé, Carsten Prasse, Carsten Prasse, Thomas Luechtefeld, Thomas Luechtefeld, Thomas Hartung, Thomas Hartung   +6 more
doaj   +1 more source

Alternatives to animal testing to assess MASH drugs and hepatotoxicity

Hepatology, 2023
The Food and Drug Administration (FDA) Modernization Act 2.0 “allows for alternatives to animal testing for purposes of drug and biological product applications.” This provides an opportunity to develop and improve alternatives to animal studies to assess drugs in the liver.
openaire   +2 more sources